Endomicrobium trichonymphae

Taxonomy: cellular organisms; Bacteria; Elusimicrobia; Endomicrobia; Endomicrobiales; Endomicrobiaceae; Endomicrobium

Average proteome isoelectric point is 7.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5IJA1|A0A1E5IJA1_ENDTX Radical SAM protein (Fragment) OS=Endomicrobium trichonymphae OX=1408204 GN=ATZ36_00005 PE=4 SV=1
MM1 pKa = 7.27TNCILFFEE9 pKa = 5.29KK10 pKa = 10.76YY11 pKa = 9.01FSEE14 pKa = 4.3SGSVYY19 pKa = 10.09FVKK22 pKa = 9.9TADD25 pKa = 3.04WQRR28 pKa = 11.84VYY30 pKa = 11.15EE31 pKa = 5.27HH32 pKa = 7.48GYY34 pKa = 8.63TDD36 pKa = 3.84NKK38 pKa = 11.07DD39 pKa = 5.16LILFWKK45 pKa = 8.16THH47 pKa = 5.07MLYY50 pKa = 10.34CVKK53 pKa = 10.71GDD55 pKa = 4.25ILFQDD60 pKa = 3.6ISTEE64 pKa = 4.05IEE66 pKa = 3.97DD67 pKa = 3.75EE68 pKa = 4.19

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5IKM6|A0A1E5IKM6_ENDTX Thiazole synthase OS=Endomicrobium trichonymphae OX=1408204 GN=ATZ36_03025 PE=4 SV=1
MM1 pKa = 7.37ATTSAEE7 pKa = 4.04AKK9 pKa = 9.08MLKK12 pKa = 8.74PQKK15 pKa = 10.12FATRR19 pKa = 11.84YY20 pKa = 9.04RR21 pKa = 11.84NRR23 pKa = 11.84CRR25 pKa = 11.84LCGRR29 pKa = 11.84PRR31 pKa = 11.84GYY33 pKa = 11.0YY34 pKa = 9.53RR35 pKa = 11.84DD36 pKa = 3.83FGICRR41 pKa = 11.84ICLRR45 pKa = 11.84KK46 pKa = 9.27LAHH49 pKa = 6.4NGEE52 pKa = 4.35IPGVTKK58 pKa = 10.78SSWW61 pKa = 2.87

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2732

0

2732

668676

37

1535

244.8

27.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.983 ± 0.049

1.36 ± 0.02

5.05 ± 0.028

6.781 ± 0.046

4.81 ± 0.041

6.407 ± 0.048

1.499 ± 0.017

9.104 ± 0.048

9.68 ± 0.051

8.85 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.397 ± 0.02

5.181 ± 0.031

3.139 ± 0.023

2.488 ± 0.026

4.192 ± 0.032

6.665 ± 0.038

4.642 ± 0.032

6.575 ± 0.038

0.688 ± 0.016

3.509 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski