Corynebacterium timonense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Corynebacteriaceae; Coryne

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1SH60|A0A1H1SH60_9CORY tRNA N6-adenosine threonylcarbamoyltransferase OS=Corynebacterium timonense OX=441500 GN=tsaD PE=3 SV=1
MM1 pKa = 7.22MPLDD5 pKa = 4.72PFADD9 pKa = 4.24DD10 pKa = 4.78PNDD13 pKa = 3.66PASFIEE19 pKa = 4.61DD20 pKa = 3.85DD21 pKa = 4.22EE22 pKa = 4.72VAQPLSPQEE31 pKa = 4.42RR32 pKa = 11.84IDD34 pKa = 3.91VIRR37 pKa = 11.84DD38 pKa = 3.44LAQVRR43 pKa = 11.84EE44 pKa = 4.84FIHH47 pKa = 6.08NLSPRR52 pKa = 11.84GILGIYY58 pKa = 7.34FTCEE62 pKa = 3.54DD63 pKa = 4.54CEE65 pKa = 4.16QVHH68 pKa = 6.04YY69 pKa = 10.52YY70 pKa = 10.28DD71 pKa = 4.06WEE73 pKa = 3.98IMEE76 pKa = 4.39QNMLASLRR84 pKa = 11.84GEE86 pKa = 4.13LPPVHH91 pKa = 6.58EE92 pKa = 5.37PSAQPNVDD100 pKa = 5.06AYY102 pKa = 8.71VTWDD106 pKa = 3.49YY107 pKa = 11.97ALGYY111 pKa = 9.98IDD113 pKa = 4.95GLRR116 pKa = 11.84ARR118 pKa = 4.78

Molecular weight: 13.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1TCN8|A0A1H1TCN8_9CORY dTDP-glucose 4 6-dehydratase OS=Corynebacterium timonense OX=441500 GN=SAMN04488539_1950 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.63KK15 pKa = 9.39KK16 pKa = 9.04HH17 pKa = 5.39RR18 pKa = 11.84KK19 pKa = 5.37MLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2311

0

2311

750681

26

2983

324.8

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.735 ± 0.072

0.668 ± 0.011

6.018 ± 0.04

6.35 ± 0.055

3.155 ± 0.031

8.597 ± 0.046

2.094 ± 0.024

4.47 ± 0.039

2.499 ± 0.037

9.537 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.022

2.579 ± 0.031

5.334 ± 0.038

2.977 ± 0.027

6.882 ± 0.049

5.613 ± 0.034

6.244 ± 0.04

8.595 ± 0.052

1.379 ± 0.019

2.233 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski