Faeces associated gemycircularvirus 21
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A161IBB7|A0A161IBB7_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 21 OX=1843741 PE=3 SV=1
MM1 pKa = 7.34 TFAINAKK8 pKa = 9.8 YY9 pKa = 10.82 VLLTYY14 pKa = 9.11 AQCGDD19 pKa = 3.65 LDD21 pKa = 4.01 GFAVMDD27 pKa = 5.51 RR28 pKa = 11.84 ISEE31 pKa = 4.19 LGGEE35 pKa = 4.48 CIVARR40 pKa = 11.84 EE41 pKa = 3.98 THH43 pKa = 6.64 ADD45 pKa = 3.46 GGTHH49 pKa = 6.5 LHH51 pKa = 5.76 VFCDD55 pKa = 4.88 FGRR58 pKa = 11.84 KK59 pKa = 8.16 FRR61 pKa = 11.84 SRR63 pKa = 11.84 KK64 pKa = 7.62 TDD66 pKa = 2.99 VFDD69 pKa = 3.6 VLGYY73 pKa = 10.25 HH74 pKa = 6.77 PNIEE78 pKa = 4.21 PSRR81 pKa = 11.84 GTPEE85 pKa = 3.59 KK86 pKa = 10.76 GYY88 pKa = 10.89 DD89 pKa = 3.47 YY90 pKa = 10.69 AIKK93 pKa = 10.71 DD94 pKa = 3.36 GDD96 pKa = 4.31 VICGGLARR104 pKa = 11.84 PTTSRR109 pKa = 11.84 TGDD112 pKa = 2.98 SSANSKK118 pKa = 6.86 WTEE121 pKa = 3.6 ITSATNRR128 pKa = 11.84 DD129 pKa = 3.88 EE130 pKa = 4.4 FWEE133 pKa = 4.4 LVHH136 pKa = 8.01 LLDD139 pKa = 5.09 PKK141 pKa = 10.8 SAACSFGQLQKK152 pKa = 10.46 YY153 pKa = 9.47 CDD155 pKa = 3.4 WKK157 pKa = 10.91 FAVAPPTYY165 pKa = 9.42 TSPTGVEE172 pKa = 4.16 FDD174 pKa = 4.78 DD175 pKa = 5.19 GSIDD179 pKa = 5.11 GRR181 pKa = 11.84 LDD183 pKa = 2.97 WLQQSGVGSGQPLLGRR199 pKa = 11.84 CMSLCLYY206 pKa = 10.73 GEE208 pKa = 4.21 SRR210 pKa = 11.84 TGKK213 pKa = 7.35 TLWARR218 pKa = 11.84 SLGAHH223 pKa = 7.21 IYY225 pKa = 10.48 CVGLVSGDD233 pKa = 3.48 EE234 pKa = 4.17 CMKK237 pKa = 11.0 ADD239 pKa = 4.21 DD240 pKa = 4.31 ADD242 pKa = 3.78 YY243 pKa = 11.27 AIFDD247 pKa = 5.27 DD248 pKa = 3.68 IRR250 pKa = 11.84 GGIKK254 pKa = 9.99 FFPSFKK260 pKa = 10.33 EE261 pKa = 3.83 WLGCQAWVTVKK272 pKa = 10.5 CLYY275 pKa = 10.42 RR276 pKa = 11.84 EE277 pKa = 4.05 PKK279 pKa = 9.36 LIKK282 pKa = 9.2 WGKK285 pKa = 7.39 PSIWISNTDD294 pKa = 3.03 PRR296 pKa = 11.84 DD297 pKa = 3.62 NMEE300 pKa = 4.08 TSDD303 pKa = 3.87 VHH305 pKa = 6.6 WMNKK309 pKa = 8.26 NCIFVAVDD317 pKa = 3.45 SPIFRR322 pKa = 11.84 ANTEE326 pKa = 3.84
Molecular weight: 36.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.172
IPC2_protein 5.27
IPC_protein 5.245
Toseland 5.296
ProMoST 5.359
Dawson 5.334
Bjellqvist 5.448
Wikipedia 5.27
Rodwell 5.258
Grimsley 5.258
Solomon 5.334
Lehninger 5.308
Nozaki 5.499
DTASelect 5.715
Thurlkill 5.41
EMBOSS 5.41
Sillero 5.575
Patrickios 3.77
IPC_peptide 5.347
IPC2_peptide 5.563
IPC2.peptide.svr19 5.575
Protein with the highest isoelectric point:
>tr|A0A161IBB7|A0A161IBB7_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 21 OX=1843741 PE=3 SV=1
MM1 pKa = 7.75 AYY3 pKa = 10.21 ARR5 pKa = 11.84 RR6 pKa = 11.84 SRR8 pKa = 11.84 FPRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AFKK16 pKa = 10.3 RR17 pKa = 11.84 RR18 pKa = 11.84 SAPRR22 pKa = 11.84 RR23 pKa = 11.84 SRR25 pKa = 11.84 RR26 pKa = 11.84 YY27 pKa = 7.59 VAKK30 pKa = 10.17 RR31 pKa = 11.84 PSRR34 pKa = 11.84 TRR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AMPSRR43 pKa = 11.84 RR44 pKa = 11.84 SILNLTTTKK53 pKa = 10.42 KK54 pKa = 9.85 RR55 pKa = 11.84 DD56 pKa = 3.28 NMAPAFYY63 pKa = 10.19 RR64 pKa = 11.84 AGDD67 pKa = 3.89 GVTATPVNNGIALLATMPAYY87 pKa = 7.19 TTAHH91 pKa = 5.96 CVLWCATEE99 pKa = 4.17 RR100 pKa = 11.84 EE101 pKa = 4.42 YY102 pKa = 11.52 ASGSNMSDD110 pKa = 2.72 HH111 pKa = 6.77 GRR113 pKa = 11.84 RR114 pKa = 11.84 SHH116 pKa = 5.45 TPFYY120 pKa = 10.51 KK121 pKa = 10.64 GLSEE125 pKa = 5.04 RR126 pKa = 11.84 IRR128 pKa = 11.84 MKK130 pKa = 8.59 TTNSSPWLWRR140 pKa = 11.84 RR141 pKa = 11.84 IVFTMKK147 pKa = 10.56 GPLPEE152 pKa = 4.61 TIRR155 pKa = 11.84 TSIKK159 pKa = 10.38 VGNQYY164 pKa = 10.67 KK165 pKa = 10.44 RR166 pKa = 11.84 GITWLTDD173 pKa = 3.28 GSDD176 pKa = 3.11 VGNSVNGLVNTTLFAGSAGIDD197 pKa = 3.43 WNDD200 pKa = 2.91 VMLAKK205 pKa = 9.95 TDD207 pKa = 3.8 TQALTVMYY215 pKa = 10.45 DD216 pKa = 2.99 RR217 pKa = 11.84 TIQLKK222 pKa = 10.35 SGSDD226 pKa = 3.09 SGTIRR231 pKa = 11.84 DD232 pKa = 4.43 FKK234 pKa = 10.45 MCHH237 pKa = 6.06 PMNKK241 pKa = 8.1 TLVYY245 pKa = 10.28 AYY247 pKa = 10.02 DD248 pKa = 3.46 EE249 pKa = 4.77 SGTFDD254 pKa = 3.22 VSFTRR259 pKa = 11.84 SALGRR264 pKa = 11.84 AGMGDD269 pKa = 3.44 MYY271 pKa = 10.18 IYY273 pKa = 11.32 DD274 pKa = 4.12 MFQPHH279 pKa = 7.26 DD280 pKa = 3.85 FSVDD284 pKa = 2.88 GGTIEE289 pKa = 4.65 FNPKK293 pKa = 8.25 ATLYY297 pKa = 9.09 WHH299 pKa = 6.97 EE300 pKa = 4.28 KK301 pKa = 9.3
Molecular weight: 34.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.794
IPC_protein 10.599
Toseland 10.54
ProMoST 10.306
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.891
Grimsley 10.76
Solomon 10.774
Lehninger 10.73
Nozaki 10.526
DTASelect 10.409
Thurlkill 10.57
EMBOSS 10.95
Sillero 10.613
Patrickios 10.54
IPC_peptide 10.774
IPC2_peptide 9.385
IPC2.peptide.svr19 8.515
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
860
233
326
286.7
31.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.093 ± 0.403
2.674 ± 0.793
7.442 ± 0.818
3.605 ± 0.604
4.419 ± 0.242
9.186 ± 0.924
2.093 ± 0.045
4.419 ± 0.367
5.0 ± 0.281
7.093 ± 0.786
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.558 ± 0.969
3.023 ± 0.629
4.884 ± 0.404
1.977 ± 0.307
7.558 ± 1.701
7.674 ± 0.287
8.023 ± 0.929
5.349 ± 0.333
2.326 ± 0.345
3.605 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here