Termite associated circular virus 3
Average proteome isoelectric point is 9.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P1E318|A0A2P1E318_9VIRU Putative capsid protein OS=Termite associated circular virus 3 OX=2108551 PE=4 SV=1
MM1 pKa = 7.72 PFRR4 pKa = 11.84 WQGKK8 pKa = 7.07 YY9 pKa = 10.68 LFLTYY14 pKa = 9.87 PRR16 pKa = 11.84 SDD18 pKa = 3.38 FSLDD22 pKa = 3.16 EE23 pKa = 4.17 SLHH26 pKa = 5.44 FFRR29 pKa = 11.84 SQAKK33 pKa = 7.85 QATFIRR39 pKa = 11.84 VSSEE43 pKa = 3.39 RR44 pKa = 11.84 HH45 pKa = 5.41 EE46 pKa = 5.3 DD47 pKa = 3.71 GSPHH51 pKa = 5.63 RR52 pKa = 11.84 HH53 pKa = 4.84 VFIAFDD59 pKa = 3.38 KK60 pKa = 10.82 RR61 pKa = 11.84 FSFTNEE67 pKa = 2.9 RR68 pKa = 11.84 RR69 pKa = 11.84 FDD71 pKa = 3.46 FRR73 pKa = 11.84 EE74 pKa = 3.67 RR75 pKa = 11.84 HH76 pKa = 5.91 PNIQSRR82 pKa = 11.84 VDD84 pKa = 3.58 NPAACLDD91 pKa = 3.98 YY92 pKa = 11.03 VSKK95 pKa = 11.25 DD96 pKa = 3.46 GVFVDD101 pKa = 4.27 YY102 pKa = 11.4 GSVPDD107 pKa = 4.88 FNTSTSEE114 pKa = 3.75 PRR116 pKa = 11.84 EE117 pKa = 3.9 SRR119 pKa = 11.84 NQLWGRR125 pKa = 11.84 LLDD128 pKa = 3.94 EE129 pKa = 4.81 ATSASHH135 pKa = 7.28 FLQLVRR141 pKa = 11.84 EE142 pKa = 3.99 NSPYY146 pKa = 11.33 DD147 pKa = 3.11 FATRR151 pKa = 11.84 VSSLFTIRR159 pKa = 11.84 TAPYY163 pKa = 8.66 AQNASSSSATLVPEE177 pKa = 4.63 RR178 pKa = 11.84 PAGRR182 pKa = 11.84 GRR184 pKa = 11.84 SGTISTSRR192 pKa = 11.84 GSSTWTSLTPRR203 pKa = 11.84 PATSSSTTARR213 pKa = 11.84 SSGSRR218 pKa = 11.84 TTSPGSGAGGEE229 pKa = 4.44 FEE231 pKa = 5.02 ATDD234 pKa = 3.48 KK235 pKa = 11.38 YY236 pKa = 10.48 RR237 pKa = 11.84 GKK239 pKa = 9.33 RR240 pKa = 11.84 TLVWQRR246 pKa = 11.84 KK247 pKa = 7.06 SCIVLCNRR255 pKa = 11.84 GLLWDD260 pKa = 3.62 WRR262 pKa = 11.84 ASEE265 pKa = 4.9 EE266 pKa = 4.39 YY267 pKa = 10.31 RR268 pKa = 11.84 DD269 pKa = 3.75 DD270 pKa = 5.43 KK271 pKa = 11.61 DD272 pKa = 3.55 WFDD275 pKa = 4.7 HH276 pKa = 6.51 NVIICDD282 pKa = 3.95 LNKK285 pKa = 10.51 KK286 pKa = 8.34 LWRR289 pKa = 11.84 YY290 pKa = 9.93 DD291 pKa = 3.12 EE292 pKa = 5.26 SYY294 pKa = 11.69
Molecular weight: 33.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.758
IPC2_protein 8.609
IPC_protein 8.668
Toseland 9.004
ProMoST 9.092
Dawson 9.385
Bjellqvist 9.297
Wikipedia 9.575
Rodwell 9.458
Grimsley 9.428
Solomon 9.472
Lehninger 9.428
Nozaki 9.341
DTASelect 9.194
Thurlkill 9.238
EMBOSS 9.487
Sillero 9.428
Patrickios 4.813
IPC_peptide 9.458
IPC2_peptide 8.2
IPC2.peptide.svr19 7.799
Protein with the highest isoelectric point:
>tr|A0A2P1E318|A0A2P1E318_9VIRU Putative capsid protein OS=Termite associated circular virus 3 OX=2108551 PE=4 SV=1
MM1 pKa = 6.95 PQRR4 pKa = 11.84 YY5 pKa = 5.29 TRR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 SRR11 pKa = 11.84 YY12 pKa = 7.54 RR13 pKa = 11.84 STRR16 pKa = 11.84 RR17 pKa = 11.84 VRR19 pKa = 11.84 KK20 pKa = 7.57 VVRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 VPKK29 pKa = 9.5 ATMKK33 pKa = 9.8 MVRR36 pKa = 11.84 SVANRR41 pKa = 11.84 QIMKK45 pKa = 9.27 ATEE48 pKa = 3.86 TKK50 pKa = 10.28 RR51 pKa = 11.84 NVRR54 pKa = 11.84 LEE56 pKa = 4.48 EE57 pKa = 3.91 NWSPIPSNSTGSQYY71 pKa = 10.97 TIANIFAQIGGGGTASGTNATQLIGDD97 pKa = 4.15 TFFNPLFKK105 pKa = 10.93 AKK107 pKa = 10.02 LRR109 pKa = 11.84 YY110 pKa = 9.45 FIDD113 pKa = 3.38 WNRR116 pKa = 11.84 VRR118 pKa = 11.84 SLNGSGTGVLPVQLYY133 pKa = 9.57 CWIVAANDD141 pKa = 3.56 ILPLGVTPNNYY152 pKa = 7.81 FTISPQPNFFLTSGGTAPTFNGDD175 pKa = 3.01 NVRR178 pKa = 11.84 VIRR181 pKa = 11.84 KK182 pKa = 7.04 WSKK185 pKa = 9.37 TINPPSIVVGAGTVVTNGASLYY207 pKa = 10.38 KK208 pKa = 10.62 KK209 pKa = 10.34 NITVKK214 pKa = 10.3 FRR216 pKa = 11.84 GSKK219 pKa = 8.64 TFEE222 pKa = 4.15 SDD224 pKa = 2.91 SSGGLGFGLRR234 pKa = 11.84 GWNFYY239 pKa = 10.26 MITGYY244 pKa = 11.49 AMPYY248 pKa = 10.19 GFALSGTPLLPLVDD262 pKa = 3.89 VRR264 pKa = 11.84 VDD266 pKa = 3.28 RR267 pKa = 11.84 YY268 pKa = 11.1 LYY270 pKa = 10.79 FKK272 pKa = 10.97 DD273 pKa = 3.47 PP274 pKa = 3.7
Molecular weight: 30.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.289
IPC2_protein 10.204
IPC_protein 11.125
Toseland 11.096
ProMoST 11.155
Dawson 11.169
Bjellqvist 10.994
Wikipedia 11.491
Rodwell 11.272
Grimsley 11.228
Solomon 11.433
Lehninger 11.359
Nozaki 11.082
DTASelect 10.994
Thurlkill 11.111
EMBOSS 11.535
Sillero 11.125
Patrickios 10.965
IPC_peptide 11.433
IPC2_peptide 10.204
IPC2.peptide.svr19 8.214
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
568
274
294
284.0
32.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.81 ± 0.215
0.88 ± 0.33
4.93 ± 1.288
3.345 ± 1.208
5.986 ± 0.328
7.394 ± 1.343
1.232 ± 0.79
4.049 ± 0.913
4.225 ± 0.567
6.514 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.232 ± 0.614
5.106 ± 1.172
5.458 ± 0.479
2.641 ± 0.055
10.211 ± 0.463
10.563 ± 2.092
8.099 ± 0.423
6.162 ± 1.197
2.289 ± 0.297
3.873 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here