Lynx canadensis faeces associated genomovirus CL3 128
Average proteome isoelectric point is 7.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CI23|A0A2Z5CI23_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL3 128 OX=2219129 PE=3 SV=1
MM1 pKa = 7.83 APFFLKK7 pKa = 10.17 NRR9 pKa = 11.84 RR10 pKa = 11.84 YY11 pKa = 10.97 VMLTYY16 pKa = 10.52 SQAGSDD22 pKa = 3.46 FDD24 pKa = 3.5 YY25 pKa = 10.94 WSIVEE30 pKa = 4.47 LLSSHH35 pKa = 6.0 GAEE38 pKa = 4.28 CIIGRR43 pKa = 11.84 EE44 pKa = 4.02 LHH46 pKa = 6.86 ADD48 pKa = 3.64 GGTHH52 pKa = 5.47 FHH54 pKa = 6.24 VFVDD58 pKa = 4.64 FGRR61 pKa = 11.84 LFQRR65 pKa = 11.84 RR66 pKa = 11.84 KK67 pKa = 8.43 TDD69 pKa = 3.35 LFDD72 pKa = 4.17 VGGKK76 pKa = 9.23 HH77 pKa = 6.12 PNIMPVWKK85 pKa = 8.88 TPGEE89 pKa = 4.12 AFDD92 pKa = 4.3 YY93 pKa = 8.57 ATKK96 pKa = 10.85 DD97 pKa = 3.15 GDD99 pKa = 3.7 IVAGGLEE106 pKa = 4.42 RR107 pKa = 11.84 PGTDD111 pKa = 3.49 CDD113 pKa = 4.09 YY114 pKa = 10.36 DD115 pKa = 3.91 TTNFWATAGASQSGEE130 pKa = 4.12 EE131 pKa = 4.25 FLHH134 pKa = 7.05 FLDD137 pKa = 4.3 QLAPRR142 pKa = 11.84 DD143 pKa = 3.75 LMRR146 pKa = 11.84 GFLQFRR152 pKa = 11.84 SYY154 pKa = 11.73 ADD156 pKa = 3.41 WKK158 pKa = 9.37 WAVAPEE164 pKa = 4.45 RR165 pKa = 11.84 YY166 pKa = 9.28 VDD168 pKa = 3.61 PPGVVFDD175 pKa = 4.33 TGHH178 pKa = 7.08 AEE180 pKa = 3.85 QLSEE184 pKa = 4.59 RR185 pKa = 11.84 KK186 pKa = 9.83 SLMLWGPSQFGKK198 pKa = 6.66 TTWARR203 pKa = 11.84 SLGNHH208 pKa = 6.2 IFFGSQFSGKK218 pKa = 9.73 LALDD222 pKa = 4.0 GMDD225 pKa = 3.8 DD226 pKa = 3.34 AQYY229 pKa = 11.33 AIFDD233 pKa = 3.77 DD234 pKa = 3.97 WKK236 pKa = 10.99 GGMKK240 pKa = 10.47 ALPGYY245 pKa = 9.93 KK246 pKa = 9.62 DD247 pKa = 2.93 WFGCQWQISVRR258 pKa = 11.84 KK259 pKa = 8.42 LHH261 pKa = 6.73 HH262 pKa = 7.08 DD263 pKa = 3.06 ARR265 pKa = 11.84 LITWGRR271 pKa = 11.84 PIIWLCNKK279 pKa = 9.89 DD280 pKa = 3.61 PRR282 pKa = 11.84 LMHH285 pKa = 6.99 IATDD289 pKa = 3.82 DD290 pKa = 4.26 VDD292 pKa = 4.07 WEE294 pKa = 4.3 WMDD297 pKa = 3.67 EE298 pKa = 3.69 NVMFVEE304 pKa = 4.79 IVRR307 pKa = 11.84 PLVTFHH313 pKa = 7.37 ANTT316 pKa = 3.62
Molecular weight: 36.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.47
IPC2_protein 5.334
IPC_protein 5.359
Toseland 5.728
ProMoST 5.677
Dawson 5.575
Bjellqvist 5.614
Wikipedia 5.563
Rodwell 5.537
Grimsley 5.881
Solomon 5.575
Lehninger 5.563
Nozaki 5.804
DTASelect 6.02
Thurlkill 5.944
EMBOSS 5.919
Sillero 5.893
Patrickios 4.139
IPC_peptide 5.588
IPC2_peptide 5.906
IPC2.peptide.svr19 5.871
Protein with the highest isoelectric point:
>tr|A0A2Z5CI23|A0A2Z5CI23_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL3 128 OX=2219129 PE=3 SV=1
MM1 pKa = 7.83 AYY3 pKa = 9.73 KK4 pKa = 10.34 SYY6 pKa = 11.19 AKK8 pKa = 10.04 RR9 pKa = 11.84 RR10 pKa = 11.84 PTRR13 pKa = 11.84 RR14 pKa = 11.84 SYY16 pKa = 10.39 KK17 pKa = 9.4 SKK19 pKa = 10.33 RR20 pKa = 11.84 RR21 pKa = 11.84 SAPRR25 pKa = 11.84 RR26 pKa = 11.84 SYY28 pKa = 10.87 AKK30 pKa = 10.01 KK31 pKa = 10.08 RR32 pKa = 11.84 STFPSRR38 pKa = 11.84 RR39 pKa = 11.84 MSKK42 pKa = 10.44 KK43 pKa = 9.98 KK44 pKa = 9.74 ILNVVSRR51 pKa = 11.84 KK52 pKa = 9.29 KK53 pKa = 10.47 RR54 pKa = 11.84 NGMMSWSNTTSSSGASQSIAAGNAVVAGNSGGTFLFSPTCMNLNQGTTSPNYY106 pKa = 9.95 AINVAEE112 pKa = 4.33 RR113 pKa = 11.84 TATTCYY119 pKa = 9.81 MKK121 pKa = 10.7 GFKK124 pKa = 10.32 EE125 pKa = 4.11 NIRR128 pKa = 11.84 IQTNSHH134 pKa = 6.26 LPWFHH139 pKa = 6.69 RR140 pKa = 11.84 RR141 pKa = 11.84 ICFRR145 pKa = 11.84 YY146 pKa = 9.17 RR147 pKa = 11.84 GSGPFTTANPVDD159 pKa = 4.11 TPTNTVTPYY168 pKa = 10.26 IDD170 pKa = 3.37 TSNGMEE176 pKa = 4.19 RR177 pKa = 11.84 LWLNQQVNNMPQTLVVLNQILFKK200 pKa = 10.95 GSQNQDD206 pKa = 2.32 WNDD209 pKa = 3.13 IVIAPVDD216 pKa = 3.6 TARR219 pKa = 11.84 VDD221 pKa = 4.02 LVFDD225 pKa = 3.76 KK226 pKa = 10.78 TWLIKK231 pKa = 10.64 SGNEE235 pKa = 3.29 TGTILEE241 pKa = 4.16 RR242 pKa = 11.84 KK243 pKa = 8.6 LWHH246 pKa = 6.39 GMNKK250 pKa = 9.72 NLVFDD255 pKa = 4.85 DD256 pKa = 4.7 DD257 pKa = 5.07 EE258 pKa = 6.25 IGEE261 pKa = 4.57 SMNSNYY267 pKa = 9.84 FSVDD271 pKa = 2.98 SKK273 pKa = 11.36 RR274 pKa = 11.84 GMGDD278 pKa = 3.13 FYY280 pKa = 10.57 IYY282 pKa = 10.43 DD283 pKa = 3.6 IVQPGLGGGATDD295 pKa = 6.39 LISLSANSTMYY306 pKa = 9.46 WHH308 pKa = 7.07 EE309 pKa = 4.05 KK310 pKa = 9.5
Molecular weight: 35.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.234
IPC2_protein 9.531
IPC_protein 9.692
Toseland 10.189
ProMoST 9.897
Dawson 10.379
Bjellqvist 10.058
Wikipedia 10.555
Rodwell 10.789
Grimsley 10.452
Solomon 10.409
Lehninger 10.379
Nozaki 10.189
DTASelect 10.043
Thurlkill 10.233
EMBOSS 10.599
Sillero 10.292
Patrickios 10.394
IPC_peptide 10.409
IPC2_peptide 8.77
IPC2.peptide.svr19 8.551
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
626
310
316
313.0
35.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.39 ± 0.608
1.118 ± 0.101
6.869 ± 1.579
3.355 ± 0.519
5.591 ± 1.154
8.147 ± 0.705
2.556 ± 0.849
4.633 ± 0.355
5.431 ± 0.685
6.709 ± 0.822
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.514 ± 0.239
5.272 ± 2.09
4.313 ± 0.08
3.355 ± 0.086
6.55 ± 0.584
7.348 ± 1.779
6.709 ± 1.126
5.431 ± 0.035
3.355 ± 0.736
3.355 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here