Hahella chejuensis (strain KCTC 2396)
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6751 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2SI75|Q2SI75_HAHCH Uncharacterized protein OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=HCH_02873 PE=4 SV=1
MM1 pKa = 7.83 PEE3 pKa = 3.69 YY4 pKa = 10.41 GYY6 pKa = 10.82 HH7 pKa = 5.83 NEE9 pKa = 4.16 SFASVLFIQAGKK21 pKa = 8.22 AQSMAVGDD29 pKa = 4.08 VFFLNPGDD37 pKa = 4.24 SVDD40 pKa = 4.26 EE41 pKa = 4.36 IYY43 pKa = 10.98 AALKK47 pKa = 9.8 AASGTSNGRR56 pKa = 11.84 IGLYY60 pKa = 9.63 ICAADD65 pKa = 4.34 KK66 pKa = 10.86 LPQVGASPVRR76 pKa = 11.84 YY77 pKa = 9.04 IDD79 pKa = 3.53 IPTISTAGAVYY90 pKa = 10.26 GGAITPYY97 pKa = 10.13 VEE99 pKa = 3.85 PAGAGSFCAIVAGAIPGTSNTNLNAAKK126 pKa = 10.11 HH127 pKa = 5.71 PSDD130 pKa = 3.46 VVTSSVCNSGVGVWVRR146 pKa = 11.84 DD147 pKa = 3.59 SEE149 pKa = 5.03 DD150 pKa = 3.8 PDD152 pKa = 4.67 RR153 pKa = 11.84 LAAWAIVTLSSTPSPSISGLVGGNASTQYY182 pKa = 11.33 GDD184 pKa = 3.18 EE185 pKa = 4.31 TANVTDD191 pKa = 4.5 FSEE194 pKa = 4.95 TITSGYY200 pKa = 7.64 LTPDD204 pKa = 3.26 AGSTQYY210 pKa = 12.03 ALLDD214 pKa = 3.55 VTDD217 pKa = 4.43 NLDD220 pKa = 3.21 GTVDD224 pKa = 3.5 YY225 pKa = 10.28 RR226 pKa = 11.84 SPGDD230 pKa = 3.63 APTSAVDD237 pKa = 3.96 LVLSGASEE245 pKa = 4.29 SATLAGVAHH254 pKa = 6.44 TQTHH258 pKa = 5.77 TAQFPAPGTNADD270 pKa = 4.49 DD271 pKa = 3.99 NSFAAGQGGGNNPWFEE287 pKa = 4.22 PPAFPLADD295 pKa = 3.39 TTATYY300 pKa = 11.37 DD301 pKa = 3.65 LVFKK305 pKa = 10.71 DD306 pKa = 4.05 GVSAITTANIVLDD319 pKa = 3.89 VNDD322 pKa = 3.45 VVEE325 pKa = 4.67 AEE327 pKa = 4.28 EE328 pKa = 4.39 GTPAGTTFQVTFQKK342 pKa = 10.39 IYY344 pKa = 10.95 DD345 pKa = 4.43 DD346 pKa = 3.92 GTTASWTVTLTVGEE360 pKa = 4.63 DD361 pKa = 3.35 GSLEE365 pKa = 4.0 VDD367 pKa = 3.95 SIMGPVFKK375 pKa = 10.66 DD376 pKa = 2.58 IFRR379 pKa = 11.84 PVFRR383 pKa = 11.84 EE384 pKa = 4.05 VAA386 pKa = 3.32
Molecular weight: 39.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.834
IPC_protein 3.859
Toseland 3.63
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.228
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.973
Patrickios 1.252
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|Q2SHB7|Q2SHB7_HAHCH Transposase and inactivated derivatives OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=HCH_03196 PE=4 SV=1
MM1 pKa = 7.2 TSWRR5 pKa = 11.84 PLQTPGPRR13 pKa = 11.84 GASVSRR19 pKa = 11.84 LWISHH24 pKa = 5.49 IFRR27 pKa = 11.84 VKK29 pKa = 9.53 RR30 pKa = 11.84 TT31 pKa = 3.39
Molecular weight: 3.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.974
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6751
0
6751
2111145
29
5711
312.7
34.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.734 ± 0.037
1.047 ± 0.012
5.733 ± 0.031
6.419 ± 0.031
3.902 ± 0.02
7.296 ± 0.039
2.162 ± 0.015
5.365 ± 0.027
4.465 ± 0.028
10.541 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.445 ± 0.018
3.615 ± 0.022
4.442 ± 0.024
4.227 ± 0.023
5.916 ± 0.028
6.481 ± 0.029
4.899 ± 0.032
6.822 ± 0.031
1.39 ± 0.014
3.098 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here