Phytophthora ramorum (Sudden oak death agent)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15349 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H3G7L4|H3G7L4_PHYRM Nucleolar GTP-binding protein 2 OS=Phytophthora ramorum OX=164328 PE=3 SV=1
TT1 pKa = 7.09AACTLSGTYY10 pKa = 10.22SDD12 pKa = 4.03GTDD15 pKa = 3.03VSSCSSIVISDD26 pKa = 3.36LTVPKK31 pKa = 10.63GVTLDD36 pKa = 3.67LTNVADD42 pKa = 4.17GATITFEE49 pKa = 3.92GTTTFGEE56 pKa = 4.48EE57 pKa = 4.07EE58 pKa = 3.96WDD60 pKa = 3.87GPLILLTGNDD70 pKa = 3.62LTVTGTGTLDD80 pKa = 4.34GQGSWYY86 pKa = 9.14WEE88 pKa = 3.8QGTSISKK95 pKa = 9.97PVFFRR100 pKa = 11.84LKK102 pKa = 10.44KK103 pKa = 10.33VMSSTLEE110 pKa = 3.79SFNIKK115 pKa = 9.94NSPYY119 pKa = 8.82RR120 pKa = 11.84TFSILDD126 pKa = 3.85CEE128 pKa = 4.46DD129 pKa = 3.31TTLTGLTLDD138 pKa = 4.35SSAGDD143 pKa = 3.54DD144 pKa = 3.58TAKK147 pKa = 9.69NTDD150 pKa = 3.26GFDD153 pKa = 3.3LSRR156 pKa = 11.84NVGLTISDD164 pKa = 3.69CTVYY168 pKa = 11.07NQDD171 pKa = 4.36DD172 pKa = 4.37CLAMQSSNDD181 pKa = 3.46TTFSGNTCSGGHH193 pKa = 6.71GISIGSLGGDD203 pKa = 3.09SVTEE207 pKa = 3.85SDD209 pKa = 3.54IVSGLTVKK217 pKa = 10.44NNKK220 pKa = 9.82IIDD223 pKa = 3.99SVNGIRR229 pKa = 11.84IKK231 pKa = 10.11TIIDD235 pKa = 3.96LYY237 pKa = 10.07GTVTGASYY245 pKa = 11.19TDD247 pKa = 3.31NTLSNVEE254 pKa = 3.94NAIVIHH260 pKa = 6.36SDD262 pKa = 3.35YY263 pKa = 11.08SKK265 pKa = 11.39SEE267 pKa = 3.57GGYY270 pKa = 8.8TGDD273 pKa = 3.59ATSKK277 pKa = 10.72VAITDD282 pKa = 3.23ITISGLSGTADD293 pKa = 4.47DD294 pKa = 5.47IYY296 pKa = 11.12DD297 pKa = 3.55ILVNADD303 pKa = 3.75VVSGWTFSDD312 pKa = 3.12ITVTGDD318 pKa = 2.87TGSCSGEE325 pKa = 3.86PSGVDD330 pKa = 3.26CC331 pKa = 6.25

Molecular weight: 34.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H3G5I3|H3G5I3_PHYRM 40S ribosomal protein S26 OS=Phytophthora ramorum OX=164328 PE=3 SV=1
MM1 pKa = 7.36VKK3 pKa = 10.16QKK5 pKa = 10.61NHH7 pKa = 4.55TARR10 pKa = 11.84NNTVKK15 pKa = 10.67AHH17 pKa = 6.51RR18 pKa = 11.84NGIKK22 pKa = 9.98KK23 pKa = 9.79PKK25 pKa = 8.2NHH27 pKa = 6.98RR28 pKa = 11.84FHH30 pKa = 6.43STRR33 pKa = 11.84GVV35 pKa = 2.99

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15349

0

15349

7381725

34

10810

480.9

53.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.424 ± 0.02

1.56 ± 0.009

5.828 ± 0.013

6.695 ± 0.023

3.734 ± 0.014

6.369 ± 0.021

2.292 ± 0.008

3.83 ± 0.013

5.029 ± 0.017

9.27 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.009

3.335 ± 0.009

4.75 ± 0.017

4.2 ± 0.017

6.122 ± 0.018

8.089 ± 0.029

5.924 ± 0.019

7.25 ± 0.016

1.222 ± 0.006

2.61 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski