Ranid herpesvirus 1 (strain McKinnell) (RaHV-1)
Average proteome isoelectric point is 7.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 132 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9YQZ9|Q9YQZ9_RAHV1 ORF69 OS=Ranid herpesvirus 1 (strain McKinnell) OX=1283342 PE=4 SV=1
MM1 pKa = 7.49 EE2 pKa = 5.64 SSLEE6 pKa = 3.97 MSSEE10 pKa = 3.96 DD11 pKa = 4.0 QEE13 pKa = 4.87 NIAAGHH19 pKa = 6.88 EE20 pKa = 3.99 IQIIADD26 pKa = 3.48 AFVSNGYY33 pKa = 10.26 LSRR36 pKa = 11.84 TQAFMATCVGMLMASIGQEE55 pKa = 3.89 FEE57 pKa = 3.7 AAEE60 pKa = 4.24 PRR62 pKa = 11.84 DD63 pKa = 3.86 VDD65 pKa = 4.11 YY66 pKa = 11.01 MFDD69 pKa = 3.73 DD70 pKa = 4.06 RR71 pKa = 11.84 TDD73 pKa = 3.37 EE74 pKa = 4.8 AVDD77 pKa = 3.56 HH78 pKa = 6.11 MLHH81 pKa = 7.07 LFLDD85 pKa = 4.8 DD86 pKa = 4.3 AAHH89 pKa = 7.27 AYY91 pKa = 8.89 AKK93 pKa = 10.78 CEE95 pKa = 3.96 LDD97 pKa = 3.33 EE98 pKa = 4.54 FLAGSAEE105 pKa = 4.04 EE106 pKa = 4.16 QEE108 pKa = 4.47 RR109 pKa = 11.84 EE110 pKa = 3.95 LAEE113 pKa = 4.29 EE114 pKa = 4.58 GSVIVEE120 pKa = 4.81 DD121 pKa = 4.3 YY122 pKa = 10.95 PLTDD126 pKa = 3.09 VTGFAVFTPSSDD138 pKa = 3.29 APIVIVGVCKK148 pKa = 9.84 DD149 pKa = 3.63 TSWRR153 pKa = 11.84 HH154 pKa = 4.82 ADD156 pKa = 3.05 IANLVHH162 pKa = 6.65 RR163 pKa = 11.84 VLMSS167 pKa = 3.57
Molecular weight: 18.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.033
IPC2_protein 4.024
IPC_protein 3.986
Toseland 3.795
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.101
Wikipedia 3.846
Rodwell 3.821
Grimsley 3.706
Solomon 3.948
Lehninger 3.897
Nozaki 4.062
DTASelect 4.253
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.101
Patrickios 2.982
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 3.992
Protein with the highest isoelectric point:
>tr|Q14VR3|Q14VR3_RAHV1 ORF45 OS=Ranid herpesvirus 1 (strain McKinnell) OX=1283342 PE=4 SV=1
MM1 pKa = 7.32 SRR3 pKa = 11.84 LMLTGVRR10 pKa = 11.84 PSASPVSMHH19 pKa = 6.32 TPAAHH24 pKa = 6.69 CAPSSSAVVHH34 pKa = 5.82 LHH36 pKa = 5.79 SAAIHH41 pKa = 4.97 MAKK44 pKa = 10.16 AVSTCPGARR53 pKa = 11.84 SKK55 pKa = 10.94 AARR58 pKa = 11.84 NSSLIGSTNRR68 pKa = 11.84 LPTQPITSAHH78 pKa = 6.35 IPRR81 pKa = 11.84 TSGDD85 pKa = 3.14 TSSPPNSSTILATYY99 pKa = 9.11 KK100 pKa = 10.65 ARR102 pKa = 11.84 AISSNDD108 pKa = 2.51 IPRR111 pKa = 11.84 FYY113 pKa = 10.92 GGRR116 pKa = 11.84 YY117 pKa = 6.2 TRR119 pKa = 11.84 ARR121 pKa = 11.84 EE122 pKa = 3.46 QAQYY126 pKa = 10.65 AKK128 pKa = 9.9 HH129 pKa = 5.37 GVGMPRR135 pKa = 11.84 RR136 pKa = 11.84 LRR138 pKa = 11.84 AALWDD143 pKa = 3.66 RR144 pKa = 11.84 TVCSAHH150 pKa = 6.95 RR151 pKa = 11.84 RR152 pKa = 11.84 ILCAFWEE159 pKa = 4.4 AMNVLYY165 pKa = 10.54 KK166 pKa = 11.15 DD167 pKa = 3.94 NLDD170 pKa = 3.75 SLALHH175 pKa = 6.48 YY176 pKa = 10.92 YY177 pKa = 10.54 SLTSQCLSLRR187 pKa = 11.84 TTAEE191 pKa = 3.79 SSTVFALRR199 pKa = 11.84 RR200 pKa = 11.84 ALGMPDD206 pKa = 3.49 PANSFHH212 pKa = 6.2 QAVARR217 pKa = 11.84 LHH219 pKa = 6.12 CTTSVPYY226 pKa = 7.93 TTTTFTHH233 pKa = 6.72 KK234 pKa = 9.92 PDD236 pKa = 4.36 PPHH239 pKa = 6.59 PRR241 pKa = 11.84 PDD243 pKa = 4.7 RR244 pKa = 11.84 IHH246 pKa = 6.66 CALYY250 pKa = 10.51 VPPTNKK256 pKa = 10.17 APVWEE261 pKa = 4.56 TKK263 pKa = 7.35 TEE265 pKa = 3.98 RR266 pKa = 11.84 LVWVSALVLLAAQGDD281 pKa = 4.23 TALLAAQASFLAWTARR297 pKa = 11.84 TIIPFF302 pKa = 3.75
Molecular weight: 32.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.648
IPC_protein 10.423
Toseland 10.277
ProMoST 10.087
Dawson 10.482
Bjellqvist 10.262
Wikipedia 10.716
Rodwell 10.628
Grimsley 10.57
Solomon 10.555
Lehninger 10.496
Nozaki 10.335
DTASelect 10.233
Thurlkill 10.335
EMBOSS 10.687
Sillero 10.423
Patrickios 10.189
IPC_peptide 10.54
IPC2_peptide 9.531
IPC2.peptide.svr19 8.417
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
132
0
132
59898
110
3149
453.8
50.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.02 ± 0.18
3.257 ± 0.156
4.282 ± 0.133
5.164 ± 0.226
2.873 ± 0.114
5.748 ± 0.132
3.655 ± 0.096
3.706 ± 0.135
2.841 ± 0.114
9.585 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.523 ± 0.1
3.251 ± 0.093
6.625 ± 0.442
3.78 ± 0.16
7.433 ± 0.163
6.611 ± 0.361
6.84 ± 0.179
6.92 ± 0.179
1.362 ± 0.074
3.524 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here