Siegesbeckia yellow vein virus-[GD13]-associated DNA beta
Average proteome isoelectric point is 4.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q14RT6|Q14RT6_9VIRU Putative C1 protein OS=Siegesbeckia yellow vein virus-[GD13]-associated DNA beta OX=371411 GN=C1 PE=4 SV=1
MM1 pKa = 7.72 TITYY5 pKa = 10.53 NNGKK9 pKa = 9.86 GVTFTINVRR18 pKa = 11.84 ISPNLKK24 pKa = 8.18 VHH26 pKa = 7.0 LRR28 pKa = 11.84 MLCTNEE34 pKa = 3.92 PVMSRR39 pKa = 11.84 YY40 pKa = 10.33 NYY42 pKa = 8.93 ILPYY46 pKa = 8.54 EE47 pKa = 4.7 HH48 pKa = 7.55 EE49 pKa = 5.75 DD50 pKa = 3.02 IFPPFDD56 pKa = 3.51 INGTEE61 pKa = 4.04 EE62 pKa = 4.07 AVKK65 pKa = 8.94 EE66 pKa = 4.42 TIRR69 pKa = 11.84 IMTEE73 pKa = 3.35 GVYY76 pKa = 10.7 FKK78 pKa = 10.75 DD79 pKa = 3.02 ITKK82 pKa = 10.8 EE83 pKa = 3.93 EE84 pKa = 4.35 LLDD87 pKa = 4.92 SIDD90 pKa = 2.96 TMMIEE95 pKa = 4.1 RR96 pKa = 11.84 FQFIEE101 pKa = 4.59 LDD103 pKa = 3.81 TKK105 pKa = 11.16 GVTSVTSRR113 pKa = 11.84 CTLL116 pKa = 3.29
Molecular weight: 13.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.928
IPC2_protein 5.041
IPC_protein 4.889
Toseland 4.787
ProMoST 4.991
Dawson 4.838
Bjellqvist 4.978
Wikipedia 4.685
Rodwell 4.749
Grimsley 4.698
Solomon 4.838
Lehninger 4.787
Nozaki 4.952
DTASelect 5.054
Thurlkill 4.774
EMBOSS 4.711
Sillero 5.016
Patrickios 3.897
IPC_peptide 4.838
IPC2_peptide 5.016
IPC2.peptide.svr19 4.993
Protein with the highest isoelectric point:
>tr|Q14RT6|Q14RT6_9VIRU Putative C1 protein OS=Siegesbeckia yellow vein virus-[GD13]-associated DNA beta OX=371411 GN=C1 PE=4 SV=1
MM1 pKa = 7.72 TITYY5 pKa = 10.53 NNGKK9 pKa = 9.86 GVTFTINVRR18 pKa = 11.84 ISPNLKK24 pKa = 8.18 VHH26 pKa = 7.0 LRR28 pKa = 11.84 MLCTNEE34 pKa = 3.92 PVMSRR39 pKa = 11.84 YY40 pKa = 10.33 NYY42 pKa = 8.93 ILPYY46 pKa = 8.54 EE47 pKa = 4.7 HH48 pKa = 7.55 EE49 pKa = 5.75 DD50 pKa = 3.02 IFPPFDD56 pKa = 3.51 INGTEE61 pKa = 4.04 EE62 pKa = 4.07 AVKK65 pKa = 8.94 EE66 pKa = 4.42 TIRR69 pKa = 11.84 IMTEE73 pKa = 3.35 GVYY76 pKa = 10.7 FKK78 pKa = 10.75 DD79 pKa = 3.02 ITKK82 pKa = 10.8 EE83 pKa = 3.93 EE84 pKa = 4.35 LLDD87 pKa = 4.92 SIDD90 pKa = 2.96 TMMIEE95 pKa = 4.1 RR96 pKa = 11.84 FQFIEE101 pKa = 4.59 LDD103 pKa = 3.81 TKK105 pKa = 11.16 GVTSVTSRR113 pKa = 11.84 CTLL116 pKa = 3.29
Molecular weight: 13.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.928
IPC2_protein 5.041
IPC_protein 4.889
Toseland 4.787
ProMoST 4.991
Dawson 4.838
Bjellqvist 4.978
Wikipedia 4.685
Rodwell 4.749
Grimsley 4.698
Solomon 4.838
Lehninger 4.787
Nozaki 4.952
DTASelect 5.054
Thurlkill 4.774
EMBOSS 4.711
Sillero 5.016
Patrickios 3.897
IPC_peptide 4.838
IPC2_peptide 5.016
IPC2.peptide.svr19 4.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
116
116
116
116.0
13.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
0.862 ± 0.0
1.724 ± 0.0
5.172 ± 0.0
9.483 ± 0.0
5.172 ± 0.0
4.31 ± 0.0
1.724 ± 0.0
10.345 ± 0.0
5.172 ± 0.0
6.897 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.172 ± 0.0
6.034 ± 0.0
4.31 ± 0.0
0.862 ± 0.0
5.172 ± 0.0
4.31 ± 0.0
12.069 ± 0.0
6.897 ± 0.0
0.0 ± 0.0
4.31 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here