Calothrix elsteri CCALA 953

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Calotrichaceae; Calothrix; Calothrix elsteri

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5667 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2TQ78|A0A2A2TQ78_9CYAN Uncharacterized protein OS=Calothrix elsteri CCALA 953 OX=987040 GN=CK510_01515 PE=4 SV=1
MM1 pKa = 7.56SEE3 pKa = 3.53QTIYY7 pKa = 11.39DD8 pKa = 4.28EE9 pKa = 4.87ILGEE13 pKa = 4.28LVWNQDD19 pKa = 3.61DD20 pKa = 4.36EE21 pKa = 5.02CWTSQVQFTLEE32 pKa = 4.33HH33 pKa = 6.25IVNITIDD40 pKa = 3.86PEE42 pKa = 4.25DD43 pKa = 4.09VEE45 pKa = 4.55TGSIILMARR54 pKa = 11.84KK55 pKa = 9.61SFSQLQQNEE64 pKa = 4.01DD65 pKa = 3.96NIRR68 pKa = 11.84HH69 pKa = 6.57LISEE73 pKa = 4.23QMLGDD78 pKa = 4.0YY79 pKa = 10.75NEE81 pKa = 4.0NWNDD85 pKa = 3.46GEE87 pKa = 5.15KK88 pKa = 9.79IDD90 pKa = 4.02IQDD93 pKa = 4.56FIDD96 pKa = 4.23SIKK99 pKa = 11.17LEE101 pKa = 4.75DD102 pKa = 3.66IVFNANGNIQLFYY115 pKa = 11.02NDD117 pKa = 4.13GYY119 pKa = 11.33LFAGHH124 pKa = 7.33GIMVTIDD131 pKa = 3.32DD132 pKa = 3.84TGSYY136 pKa = 10.16QEE138 pKa = 5.46AILFGG143 pKa = 4.07

Molecular weight: 16.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2TAN9|A0A2A2TAN9_9CYAN DNA (cytosine-5-)-methyltransferase OS=Calothrix elsteri CCALA 953 OX=987040 GN=CK510_28940 PE=3 SV=1
MM1 pKa = 7.39KK2 pKa = 9.11RR3 pKa = 11.84TLGGTNRR10 pKa = 11.84KK11 pKa = 9.1RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPTGRR28 pKa = 11.84NVIKK32 pKa = 10.41ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.92GRR39 pKa = 11.84HH40 pKa = 4.95RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5667

0

5667

1726011

18

3806

304.6

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.646 ± 0.033

1.01 ± 0.011

4.911 ± 0.025

6.355 ± 0.034

4.078 ± 0.021

6.516 ± 0.036

1.704 ± 0.013

7.329 ± 0.029

5.453 ± 0.031

10.513 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.903 ± 0.016

4.999 ± 0.031

4.433 ± 0.026

5.077 ± 0.028

4.895 ± 0.026

6.671 ± 0.024

5.666 ± 0.03

6.422 ± 0.028

1.372 ± 0.014

3.049 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski