Amino acid dipepetide frequency for Mycobacterium phage TChen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.285AlaAla: 13.285 ± 1.571
0.922AlaCys: 0.922 ± 0.24
6.236AlaAsp: 6.236 ± 0.554
8.405AlaGlu: 8.405 ± 1.232
2.386AlaPhe: 2.386 ± 0.458
8.676AlaGly: 8.676 ± 1.088
1.247AlaHis: 1.247 ± 0.268
3.796AlaIle: 3.796 ± 0.555
4.229AlaLys: 4.229 ± 0.511
8.188AlaLeu: 8.188 ± 0.572
3.253AlaMet: 3.253 ± 0.456
3.308AlaAsn: 3.308 ± 0.594
5.314AlaPro: 5.314 ± 0.596
3.85AlaGln: 3.85 ± 0.463
7.158AlaArg: 7.158 ± 0.774
5.314AlaSer: 5.314 ± 0.693
5.26AlaThr: 5.26 ± 0.739
6.398AlaVal: 6.398 ± 0.582
2.874AlaTrp: 2.874 ± 0.476
1.627AlaTyr: 1.627 ± 0.284
0.0AlaXaa: 0.0 ± 0.0
Cys
1.193CysAla: 1.193 ± 0.319
0.054CysCys: 0.054 ± 0.055
0.596CysAsp: 0.596 ± 0.187
0.868CysGlu: 0.868 ± 0.243
0.163CysPhe: 0.163 ± 0.109
1.301CysGly: 1.301 ± 0.337
0.271CysHis: 0.271 ± 0.123
0.217CysIle: 0.217 ± 0.117
0.38CysLys: 0.38 ± 0.163
0.813CysLeu: 0.813 ± 0.261
0.163CysMet: 0.163 ± 0.111
0.38CysAsn: 0.38 ± 0.15
1.301CysPro: 1.301 ± 0.31
0.38CysGln: 0.38 ± 0.135
1.084CysArg: 1.084 ± 0.286
0.922CysSer: 0.922 ± 0.245
1.03CysThr: 1.03 ± 0.284
0.488CysVal: 0.488 ± 0.174
0.271CysTrp: 0.271 ± 0.118
0.163CysTyr: 0.163 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
6.182AspAla: 6.182 ± 0.612
0.868AspCys: 0.868 ± 0.22
4.175AspAsp: 4.175 ± 0.567
3.308AspGlu: 3.308 ± 0.428
1.952AspPhe: 1.952 ± 0.318
5.965AspGly: 5.965 ± 0.6
1.41AspHis: 1.41 ± 0.279
2.711AspIle: 2.711 ± 0.38
2.061AspLys: 2.061 ± 0.359
5.26AspLeu: 5.26 ± 0.538
1.356AspMet: 1.356 ± 0.3
1.627AspAsn: 1.627 ± 0.281
4.013AspPro: 4.013 ± 0.498
3.253AspGln: 3.253 ± 0.435
4.772AspArg: 4.772 ± 0.551
3.037AspSer: 3.037 ± 0.451
3.85AspThr: 3.85 ± 0.398
4.555AspVal: 4.555 ± 0.526
1.301AspTrp: 1.301 ± 0.292
1.789AspTyr: 1.789 ± 0.362
0.0AspXaa: 0.0 ± 0.0
Glu
5.477GluAla: 5.477 ± 0.598
0.759GluCys: 0.759 ± 0.204
3.091GluAsp: 3.091 ± 0.349
2.765GluGlu: 2.765 ± 0.391
2.765GluPhe: 2.765 ± 0.392
2.657GluGly: 2.657 ± 0.51
1.193GluHis: 1.193 ± 0.276
2.332GluIle: 2.332 ± 0.44
2.061GluLys: 2.061 ± 0.399
5.748GluLeu: 5.748 ± 0.655
1.301GluMet: 1.301 ± 0.292
2.223GluAsn: 2.223 ± 0.318
2.982GluPro: 2.982 ± 0.386
3.796GluGln: 3.796 ± 0.39
4.826GluArg: 4.826 ± 0.674
3.091GluSer: 3.091 ± 0.505
4.013GluThr: 4.013 ± 0.404
4.989GluVal: 4.989 ± 0.567
1.627GluTrp: 1.627 ± 0.304
1.735GluTyr: 1.735 ± 0.389
0.0GluXaa: 0.0 ± 0.0
Phe
2.765PheAla: 2.765 ± 0.34
0.325PheCys: 0.325 ± 0.13
2.603PheAsp: 2.603 ± 0.334
2.006PheGlu: 2.006 ± 0.355
0.651PhePhe: 0.651 ± 0.173
2.874PheGly: 2.874 ± 0.466
0.705PheHis: 0.705 ± 0.154
1.681PheIle: 1.681 ± 0.306
0.976PheLys: 0.976 ± 0.213
2.82PheLeu: 2.82 ± 0.314
0.976PheMet: 0.976 ± 0.174
1.03PheAsn: 1.03 ± 0.27
1.518PhePro: 1.518 ± 0.255
1.139PheGln: 1.139 ± 0.218
1.789PheArg: 1.789 ± 0.315
1.247PheSer: 1.247 ± 0.281
2.928PheThr: 2.928 ± 0.467
1.193PheVal: 1.193 ± 0.238
0.488PheTrp: 0.488 ± 0.169
0.651PheTyr: 0.651 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
8.622GlyAla: 8.622 ± 1.462
1.084GlyCys: 1.084 ± 0.28
4.772GlyAsp: 4.772 ± 0.443
4.663GlyGlu: 4.663 ± 0.49
2.549GlyPhe: 2.549 ± 0.3
10.465GlyGly: 10.465 ± 2.53
2.006GlyHis: 2.006 ± 0.326
3.633GlyIle: 3.633 ± 0.554
2.982GlyLys: 2.982 ± 0.381
5.097GlyLeu: 5.097 ± 0.717
2.115GlyMet: 2.115 ± 0.346
3.416GlyAsn: 3.416 ± 0.523
4.067GlyPro: 4.067 ± 0.517
2.82GlyGln: 2.82 ± 0.546
6.67GlyArg: 6.67 ± 0.639
4.88GlySer: 4.88 ± 0.779
6.127GlyThr: 6.127 ± 0.813
6.995GlyVal: 6.995 ± 0.707
2.657GlyTrp: 2.657 ± 0.388
2.332GlyTyr: 2.332 ± 0.345
0.0GlyXaa: 0.0 ± 0.0
His
1.844HisAla: 1.844 ± 0.286
0.542HisCys: 0.542 ± 0.176
1.356HisAsp: 1.356 ± 0.317
1.247HisGlu: 1.247 ± 0.337
0.542HisPhe: 0.542 ± 0.153
1.681HisGly: 1.681 ± 0.339
1.03HisHis: 1.03 ± 0.307
0.868HisIle: 0.868 ± 0.233
0.922HisLys: 0.922 ± 0.236
1.464HisLeu: 1.464 ± 0.343
0.488HisMet: 0.488 ± 0.142
0.759HisAsn: 0.759 ± 0.173
1.356HisPro: 1.356 ± 0.246
1.247HisGln: 1.247 ± 0.263
1.898HisArg: 1.898 ± 0.343
0.759HisSer: 0.759 ± 0.221
1.681HisThr: 1.681 ± 0.398
1.356HisVal: 1.356 ± 0.285
0.759HisTrp: 0.759 ± 0.208
0.813HisTyr: 0.813 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
5.368IleAla: 5.368 ± 0.567
0.596IleCys: 0.596 ± 0.212
4.067IleAsp: 4.067 ± 0.51
3.687IleGlu: 3.687 ± 0.421
1.084IlePhe: 1.084 ± 0.237
3.416IleGly: 3.416 ± 0.444
0.976IleHis: 0.976 ± 0.208
1.464IleIle: 1.464 ± 0.304
0.813IleLys: 0.813 ± 0.214
1.952IleLeu: 1.952 ± 0.292
0.868IleMet: 0.868 ± 0.275
1.627IleAsn: 1.627 ± 0.279
3.037IlePro: 3.037 ± 0.439
1.301IleGln: 1.301 ± 0.25
2.657IleArg: 2.657 ± 0.381
2.006IleSer: 2.006 ± 0.283
3.199IleThr: 3.199 ± 0.545
2.874IleVal: 2.874 ± 0.391
0.868IleTrp: 0.868 ± 0.244
0.976IleTyr: 0.976 ± 0.231
0.0IleXaa: 0.0 ± 0.0
Lys
2.494LysAla: 2.494 ± 0.356
0.488LysCys: 0.488 ± 0.156
2.277LysAsp: 2.277 ± 0.42
1.247LysGlu: 1.247 ± 0.251
1.03LysPhe: 1.03 ± 0.248
3.308LysGly: 3.308 ± 0.4
0.976LysHis: 0.976 ± 0.275
1.518LysIle: 1.518 ± 0.317
1.03LysLys: 1.03 ± 0.253
2.711LysLeu: 2.711 ± 0.362
0.813LysMet: 0.813 ± 0.188
0.434LysAsn: 0.434 ± 0.122
2.332LysPro: 2.332 ± 0.469
1.139LysGln: 1.139 ± 0.221
2.711LysArg: 2.711 ± 0.39
2.549LysSer: 2.549 ± 0.438
2.061LysThr: 2.061 ± 0.356
2.982LysVal: 2.982 ± 0.408
0.705LysTrp: 0.705 ± 0.2
1.084LysTyr: 1.084 ± 0.223
0.0LysXaa: 0.0 ± 0.0
Leu
8.405LeuAla: 8.405 ± 0.687
0.542LeuCys: 0.542 ± 0.175
4.284LeuAsp: 4.284 ± 0.657
4.229LeuGlu: 4.229 ± 0.522
2.169LeuPhe: 2.169 ± 0.346
6.398LeuGly: 6.398 ± 0.596
1.301LeuHis: 1.301 ± 0.258
2.874LeuIle: 2.874 ± 0.387
2.169LeuLys: 2.169 ± 0.344
6.073LeuLeu: 6.073 ± 0.469
1.844LeuMet: 1.844 ± 0.294
2.549LeuAsn: 2.549 ± 0.385
5.043LeuPro: 5.043 ± 0.566
2.332LeuGln: 2.332 ± 0.352
5.26LeuArg: 5.26 ± 0.592
4.175LeuSer: 4.175 ± 0.506
5.748LeuThr: 5.748 ± 0.542
5.206LeuVal: 5.206 ± 0.535
1.139LeuTrp: 1.139 ± 0.302
1.952LeuTyr: 1.952 ± 0.345
0.0LeuXaa: 0.0 ± 0.0
Met
2.115MetAla: 2.115 ± 0.334
0.271MetCys: 0.271 ± 0.127
1.03MetAsp: 1.03 ± 0.192
1.084MetGlu: 1.084 ± 0.228
1.139MetPhe: 1.139 ± 0.297
1.464MetGly: 1.464 ± 0.451
0.38MetHis: 0.38 ± 0.147
0.976MetIle: 0.976 ± 0.205
0.759MetLys: 0.759 ± 0.269
1.627MetLeu: 1.627 ± 0.247
0.542MetMet: 0.542 ± 0.132
0.976MetAsn: 0.976 ± 0.278
1.139MetPro: 1.139 ± 0.218
0.705MetGln: 0.705 ± 0.214
1.518MetArg: 1.518 ± 0.25
2.603MetSer: 2.603 ± 0.392
2.711MetThr: 2.711 ± 0.364
1.789MetVal: 1.789 ± 0.308
0.488MetTrp: 0.488 ± 0.163
0.542MetTyr: 0.542 ± 0.118
0.0MetXaa: 0.0 ± 0.0
Asn
3.796AsnAla: 3.796 ± 0.391
0.217AsnCys: 0.217 ± 0.105
1.572AsnAsp: 1.572 ± 0.214
2.657AsnGlu: 2.657 ± 0.368
0.596AsnPhe: 0.596 ± 0.169
3.796AsnGly: 3.796 ± 0.429
0.868AsnHis: 0.868 ± 0.29
1.247AsnIle: 1.247 ± 0.297
0.759AsnLys: 0.759 ± 0.192
2.115AsnLeu: 2.115 ± 0.375
0.488AsnMet: 0.488 ± 0.139
1.41AsnAsn: 1.41 ± 0.245
3.199AsnPro: 3.199 ± 0.421
1.084AsnGln: 1.084 ± 0.268
2.386AsnArg: 2.386 ± 0.345
1.301AsnSer: 1.301 ± 0.233
2.549AsnThr: 2.549 ± 0.413
2.169AsnVal: 2.169 ± 0.287
0.38AsnTrp: 0.38 ± 0.148
0.705AsnTyr: 0.705 ± 0.173
0.0AsnXaa: 0.0 ± 0.0
Pro
5.856ProAla: 5.856 ± 0.623
0.38ProCys: 0.38 ± 0.155
4.772ProAsp: 4.772 ± 0.511
4.175ProGlu: 4.175 ± 0.626
2.223ProPhe: 2.223 ± 0.311
5.802ProGly: 5.802 ± 0.694
1.518ProHis: 1.518 ± 0.274
2.44ProIle: 2.44 ± 0.392
2.44ProLys: 2.44 ± 0.388
3.85ProLeu: 3.85 ± 0.356
1.464ProMet: 1.464 ± 0.331
2.223ProAsn: 2.223 ± 0.456
3.85ProPro: 3.85 ± 0.653
2.549ProGln: 2.549 ± 0.36
3.199ProArg: 3.199 ± 0.445
3.416ProSer: 3.416 ± 0.436
3.687ProThr: 3.687 ± 0.518
5.097ProVal: 5.097 ± 0.544
1.247ProTrp: 1.247 ± 0.276
1.898ProTyr: 1.898 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
4.555GlnAla: 4.555 ± 0.528
0.38GlnCys: 0.38 ± 0.17
2.115GlnAsp: 2.115 ± 0.382
1.193GlnGlu: 1.193 ± 0.308
1.356GlnPhe: 1.356 ± 0.269
3.037GlnGly: 3.037 ± 0.527
1.356GlnHis: 1.356 ± 0.254
1.681GlnIle: 1.681 ± 0.284
1.356GlnLys: 1.356 ± 0.243
3.308GlnLeu: 3.308 ± 0.442
0.813GlnMet: 0.813 ± 0.202
0.651GlnAsn: 0.651 ± 0.182
3.579GlnPro: 3.579 ± 0.504
1.627GlnGln: 1.627 ± 0.382
2.44GlnArg: 2.44 ± 0.366
2.006GlnSer: 2.006 ± 0.315
1.898GlnThr: 1.898 ± 0.303
2.549GlnVal: 2.549 ± 0.305
0.488GlnTrp: 0.488 ± 0.179
1.139GlnTyr: 1.139 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
6.073ArgAla: 6.073 ± 0.671
1.464ArgCys: 1.464 ± 0.298
4.013ArgAsp: 4.013 ± 0.554
4.175ArgGlu: 4.175 ± 0.613
2.223ArgPhe: 2.223 ± 0.328
4.555ArgGly: 4.555 ± 0.399
2.006ArgHis: 2.006 ± 0.365
4.013ArgIle: 4.013 ± 0.47
2.765ArgLys: 2.765 ± 0.467
5.422ArgLeu: 5.422 ± 0.618
2.277ArgMet: 2.277 ± 0.431
2.603ArgAsn: 2.603 ± 0.423
4.067ArgPro: 4.067 ± 0.549
2.006ArgGln: 2.006 ± 0.374
6.507ArgArg: 6.507 ± 0.841
4.826ArgSer: 4.826 ± 0.551
3.796ArgThr: 3.796 ± 0.543
4.989ArgVal: 4.989 ± 0.463
1.789ArgTrp: 1.789 ± 0.34
2.277ArgTyr: 2.277 ± 0.343
0.0ArgXaa: 0.0 ± 0.0
Ser
5.965SerAla: 5.965 ± 1.167
0.434SerCys: 0.434 ± 0.177
3.85SerAsp: 3.85 ± 0.422
2.82SerGlu: 2.82 ± 0.408
1.681SerPhe: 1.681 ± 0.249
6.561SerGly: 6.561 ± 1.09
1.03SerHis: 1.03 ± 0.246
2.44SerIle: 2.44 ± 0.372
2.332SerLys: 2.332 ± 0.371
3.85SerLeu: 3.85 ± 0.444
1.627SerMet: 1.627 ± 0.314
1.789SerAsn: 1.789 ± 0.273
3.416SerPro: 3.416 ± 0.392
1.898SerGln: 1.898 ± 0.28
3.633SerArg: 3.633 ± 0.547
3.47SerSer: 3.47 ± 0.542
3.579SerThr: 3.579 ± 0.412
3.416SerVal: 3.416 ± 0.423
1.301SerTrp: 1.301 ± 0.298
1.518SerTyr: 1.518 ± 0.287
0.0SerXaa: 0.0 ± 0.0
Thr
5.91ThrAla: 5.91 ± 0.69
0.868ThrCys: 0.868 ± 0.301
3.741ThrAsp: 3.741 ± 0.392
4.121ThrGlu: 4.121 ± 0.404
2.223ThrPhe: 2.223 ± 0.316
6.561ThrGly: 6.561 ± 0.967
1.681ThrHis: 1.681 ± 0.311
3.741ThrIle: 3.741 ± 0.541
1.681ThrLys: 1.681 ± 0.281
4.392ThrLeu: 4.392 ± 0.562
1.139ThrMet: 1.139 ± 0.309
2.277ThrAsn: 2.277 ± 0.345
5.477ThrPro: 5.477 ± 0.496
2.169ThrGln: 2.169 ± 0.389
4.338ThrArg: 4.338 ± 0.546
3.85ThrSer: 3.85 ± 0.52
4.067ThrThr: 4.067 ± 0.461
6.182ThrVal: 6.182 ± 0.715
1.41ThrTrp: 1.41 ± 0.305
1.518ThrTyr: 1.518 ± 0.296
0.0ThrXaa: 0.0 ± 0.0
Val
7.266ValAla: 7.266 ± 0.742
1.084ValCys: 1.084 ± 0.232
5.314ValAsp: 5.314 ± 0.519
4.717ValGlu: 4.717 ± 0.523
1.952ValPhe: 1.952 ± 0.357
5.856ValGly: 5.856 ± 0.685
1.518ValHis: 1.518 ± 0.288
3.253ValIle: 3.253 ± 0.472
2.549ValLys: 2.549 ± 0.366
5.585ValLeu: 5.585 ± 0.518
1.301ValMet: 1.301 ± 0.273
2.711ValAsn: 2.711 ± 0.389
3.796ValPro: 3.796 ± 0.424
2.386ValGln: 2.386 ± 0.307
4.609ValArg: 4.609 ± 0.51
4.175ValSer: 4.175 ± 0.68
5.639ValThr: 5.639 ± 0.59
5.422ValVal: 5.422 ± 0.502
1.572ValTrp: 1.572 ± 0.25
1.193ValTyr: 1.193 ± 0.246
0.0ValXaa: 0.0 ± 0.0
Trp
2.006TrpAla: 2.006 ± 0.31
0.596TrpCys: 0.596 ± 0.192
1.735TrpAsp: 1.735 ± 0.362
0.759TrpGlu: 0.759 ± 0.219
0.813TrpPhe: 0.813 ± 0.204
1.301TrpGly: 1.301 ± 0.268
0.651TrpHis: 0.651 ± 0.171
0.868TrpIle: 0.868 ± 0.168
1.03TrpLys: 1.03 ± 0.223
1.627TrpLeu: 1.627 ± 0.245
0.651TrpMet: 0.651 ± 0.176
0.813TrpAsn: 0.813 ± 0.203
1.03TrpPro: 1.03 ± 0.218
0.759TrpGln: 0.759 ± 0.208
1.518TrpArg: 1.518 ± 0.343
1.735TrpSer: 1.735 ± 0.391
2.006TrpThr: 2.006 ± 0.417
1.518TrpVal: 1.518 ± 0.322
0.705TrpTrp: 0.705 ± 0.185
0.488TrpTyr: 0.488 ± 0.185
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.549TyrAla: 2.549 ± 0.326
0.163TyrCys: 0.163 ± 0.087
1.844TyrAsp: 1.844 ± 0.296
1.301TyrGlu: 1.301 ± 0.259
0.868TyrPhe: 0.868 ± 0.276
2.277TyrGly: 2.277 ± 0.361
0.488TyrHis: 0.488 ± 0.12
1.084TyrIle: 1.084 ± 0.224
0.596TyrLys: 0.596 ± 0.174
1.789TyrLeu: 1.789 ± 0.32
0.325TyrMet: 0.325 ± 0.139
0.488TyrAsn: 0.488 ± 0.173
1.572TyrPro: 1.572 ± 0.279
1.084TyrGln: 1.084 ± 0.208
2.928TyrArg: 2.928 ± 0.378
1.247TyrSer: 1.247 ± 0.24
1.518TyrThr: 1.518 ± 0.232
1.735TyrVal: 1.735 ± 0.35
0.542TyrTrp: 0.542 ± 0.185
0.542TyrTyr: 0.542 ± 0.13
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (18443 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski