Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Chromohalobacter; Chromohalobacter salexigens

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3298 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1QWR2|Q1QWR2_CHRSD FAD linked oxidase-like protein OS=Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11) OX=290398 GN=Csal_1744 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.47KK3 pKa = 8.85STTGLLLGSALVVGLSGCASSGTQSSYY30 pKa = 11.54NADD33 pKa = 5.04DD34 pKa = 3.9DD35 pKa = 4.29QAWYY39 pKa = 10.22KK40 pKa = 11.18SPFVCGLAGGLIGGGIGYY58 pKa = 10.24ASSSDD63 pKa = 3.42SDD65 pKa = 3.79EE66 pKa = 4.35DD67 pKa = 3.33TGAALGGVGGATAGALLCADD87 pKa = 4.11YY88 pKa = 11.03SDD90 pKa = 4.99KK91 pKa = 11.39VVDD94 pKa = 3.81SDD96 pKa = 4.55GDD98 pKa = 3.99GVPDD102 pKa = 6.13DD103 pKa = 5.8RR104 pKa = 11.84DD105 pKa = 3.43QCPNTPAGVAVDD117 pKa = 4.09AQGCPLDD124 pKa = 3.85SDD126 pKa = 4.27GDD128 pKa = 4.11GVPDD132 pKa = 4.67YY133 pKa = 11.12KK134 pKa = 10.96DD135 pKa = 3.44EE136 pKa = 4.96CPGTPAGVEE145 pKa = 4.39VNASGCPLDD154 pKa = 3.97SDD156 pKa = 4.3GDD158 pKa = 4.25GVPDD162 pKa = 4.49YY163 pKa = 11.09QDD165 pKa = 3.21QCPDD169 pKa = 3.29TPAGAEE175 pKa = 4.15VNALGCAADD184 pKa = 5.1LVLKK188 pKa = 10.41DD189 pKa = 3.72VNFEE193 pKa = 3.99FDD195 pKa = 3.45SATLTSEE202 pKa = 4.59AEE204 pKa = 4.14NILDD208 pKa = 4.24DD209 pKa = 4.12VAAKK213 pKa = 10.48LSSNEE218 pKa = 4.11SVDD221 pKa = 3.5VRR223 pKa = 11.84LEE225 pKa = 4.28GYY227 pKa = 8.22TDD229 pKa = 3.67SVGSASYY236 pKa = 11.21NKK238 pKa = 10.19DD239 pKa = 2.87LSQRR243 pKa = 11.84RR244 pKa = 11.84ADD246 pKa = 3.58SVKK249 pKa = 10.27EE250 pKa = 3.78YY251 pKa = 10.77LVSKK255 pKa = 10.04GVNADD260 pKa = 3.63SITTYY265 pKa = 10.83GYY267 pKa = 11.28GEE269 pKa = 4.29QNPIATNEE277 pKa = 4.15TAEE280 pKa = 3.95GRR282 pKa = 11.84AEE284 pKa = 3.86NRR286 pKa = 11.84RR287 pKa = 11.84VEE289 pKa = 4.45LGEE292 pKa = 3.96QEE294 pKa = 4.04

Molecular weight: 29.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1QSE4|Q1QSE4_CHRSD Uncharacterized protein OS=Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11) OX=290398 GN=Csal_3270 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3298

0

3298

1099637

37

3314

333.4

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.512 ± 0.048

0.955 ± 0.015

6.111 ± 0.047

6.204 ± 0.047

3.443 ± 0.033

8.127 ± 0.039

2.551 ± 0.027

4.57 ± 0.035

2.505 ± 0.033

11.259 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.021

2.441 ± 0.027

4.903 ± 0.031

3.75 ± 0.028

7.47 ± 0.05

5.367 ± 0.035

5.221 ± 0.037

7.21 ± 0.032

1.495 ± 0.021

2.394 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski