Pseudomonas phage EL

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Elvirus; Pseudomonas virus EL

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 201 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2Z0W5|Q2Z0W5_9CAUD Uncharacterized protein OS=Pseudomonas phage EL OX=273133 PE=4 SV=1
MM1 pKa = 7.26NVQLPRR7 pKa = 11.84SVTLDD12 pKa = 3.37AYY14 pKa = 9.9LQPDD18 pKa = 4.21GTLMLGPGQAKK29 pKa = 7.91VTINYY34 pKa = 9.77HH35 pKa = 5.93SDD37 pKa = 3.17PQEE40 pKa = 4.44SINACIVFGGAMQAIVTFDD59 pKa = 3.39EE60 pKa = 4.73TGQTPVSYY68 pKa = 9.57TLLNPGGDD76 pKa = 3.69YY77 pKa = 10.46TVEE80 pKa = 3.83QSVLNILSYY89 pKa = 10.9FGVSAPMNN97 pKa = 3.94

Molecular weight: 10.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2Z0Z3|Q2Z0Z3_9CAUD Uncharacterized protein OS=Pseudomonas phage EL OX=273133 PE=4 SV=1
MM1 pKa = 7.73LGTTLPQMPCAVPLRR16 pKa = 11.84QPSCSLLSSRR26 pKa = 11.84VDD28 pKa = 2.97RR29 pKa = 11.84GFLTGVTVYY38 pKa = 10.26LFPTFLRR45 pKa = 11.84GVGNRR50 pKa = 11.84TFSLSAMCRR59 pKa = 11.84GYY61 pKa = 10.71RR62 pKa = 11.84HH63 pKa = 5.03VVHH66 pKa = 6.34YY67 pKa = 10.08KK68 pKa = 9.12GHH70 pKa = 7.03ARR72 pKa = 11.84GLWEE76 pKa = 3.55WHH78 pKa = 6.49LPP80 pKa = 3.7

Molecular weight: 9.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

201

0

201

64935

54

2543

323.1

36.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.232 ± 0.15

0.844 ± 0.056

5.971 ± 0.099

6.942 ± 0.143

4.307 ± 0.088

6.896 ± 0.225

2.051 ± 0.086

5.781 ± 0.112

5.984 ± 0.142

9.394 ± 0.144

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.338 ± 0.073

5.359 ± 0.114

4.488 ± 0.113

3.56 ± 0.075

5.211 ± 0.097

5.904 ± 0.125

6.371 ± 0.097

7.244 ± 0.135

1.25 ± 0.067

3.87 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski