Helicobacter phage Pt22899G

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RF37|A0A1S5RF37_9CAUD Uncharacterized protein OS=Helicobacter phage Pt22899G OX=1852673 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.06ASLEE25 pKa = 3.89QDD27 pKa = 4.59FIKK30 pKa = 10.97HH31 pKa = 5.29MVDD34 pKa = 2.81KK35 pKa = 10.6TNEE38 pKa = 3.95KK39 pKa = 10.6VEE41 pKa = 4.81DD42 pKa = 3.83LFFSNKK48 pKa = 8.85PEE50 pKa = 4.27FYY52 pKa = 10.1RR53 pKa = 11.84FVFTEE58 pKa = 3.6QNNYY62 pKa = 9.57LRR64 pKa = 11.84EE65 pKa = 4.0KK66 pKa = 10.13LTDD69 pKa = 3.41KK70 pKa = 10.83VGRR73 pKa = 11.84AMDD76 pKa = 4.45LSDD79 pKa = 4.48EE80 pKa = 4.15IQRR83 pKa = 11.84DD84 pKa = 3.61KK85 pKa = 11.45EE86 pKa = 4.02NEE88 pKa = 3.98EE89 pKa = 3.81IEE91 pKa = 4.14KK92 pKa = 10.98DD93 pKa = 3.25KK94 pKa = 11.4EE95 pKa = 4.14AFLKK99 pKa = 10.25KK100 pKa = 10.1HH101 pKa = 6.07PNIDD105 pKa = 3.86LNEE108 pKa = 4.16LLDD111 pKa = 4.19FYY113 pKa = 11.61NEE115 pKa = 4.28EE116 pKa = 3.61IPNRR120 pKa = 11.84IKK122 pKa = 10.86KK123 pKa = 10.37QIDD126 pKa = 3.48KK127 pKa = 11.34LEE129 pKa = 4.31GEE131 pKa = 4.73AFFEE135 pKa = 5.78AILDD139 pKa = 3.77YY140 pKa = 11.03FNAVIAKK147 pKa = 9.3EE148 pKa = 4.08EE149 pKa = 4.17EE150 pKa = 4.2PKK152 pKa = 10.48SEE154 pKa = 4.19EE155 pKa = 4.63KK156 pKa = 10.85EE157 pKa = 4.04EE158 pKa = 4.14EE159 pKa = 4.26KK160 pKa = 11.4NNLPKK165 pKa = 10.42EE166 pKa = 4.0ALGNGVSGVGYY177 pKa = 10.77ANNEE181 pKa = 4.31NIMTRR186 pKa = 11.84YY187 pKa = 9.53

Molecular weight: 22.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RF27|A0A1S5RF27_9CAUD Replicative DNA helicase OS=Helicobacter phage Pt22899G OX=1852673 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84SWNYY6 pKa = 10.46KK7 pKa = 9.61HH8 pKa = 7.11DD9 pKa = 4.13NKK11 pKa = 10.62NFKK14 pKa = 10.05PKK16 pKa = 9.74VWTNSMLLEE25 pKa = 4.59NGLLAILEE33 pKa = 4.08ALKK36 pKa = 9.76TLEE39 pKa = 4.24NKK41 pKa = 9.72SRR43 pKa = 11.84SAILEE48 pKa = 3.91RR49 pKa = 11.84LIIFYY54 pKa = 10.38IEE56 pKa = 4.19TQKK59 pKa = 11.42GEE61 pKa = 4.22SDD63 pKa = 3.06EE64 pKa = 4.62KK65 pKa = 10.1AWKK68 pKa = 9.45RR69 pKa = 11.84SKK71 pKa = 10.35RR72 pKa = 11.84AYY74 pKa = 9.52KK75 pKa = 10.24RR76 pKa = 11.84SLVSKK81 pKa = 10.41AKK83 pKa = 10.01KK84 pKa = 9.93NKK86 pKa = 9.71LKK88 pKa = 9.8RR89 pKa = 11.84TQLEE93 pKa = 4.28RR94 pKa = 11.84IAKK97 pKa = 6.9TKK99 pKa = 10.25KK100 pKa = 10.0KK101 pKa = 10.12KK102 pKa = 9.85QLQAFSDD109 pKa = 3.69RR110 pKa = 11.84AFSYY114 pKa = 10.18FEE116 pKa = 3.92RR117 pKa = 11.84AA118 pKa = 3.09

Molecular weight: 14.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

8965

41

1783

249.0

28.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.96 ± 0.366

0.647 ± 0.179

4.785 ± 0.432

8.366 ± 0.451

4.785 ± 0.331

4.094 ± 0.375

1.55 ± 0.171

6.414 ± 0.281

11.266 ± 0.463

10.987 ± 0.447

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.997 ± 0.209

8.199 ± 0.399

2.476 ± 0.19

4.718 ± 0.406

3.737 ± 0.258

6.447 ± 0.183

4.796 ± 0.287

4.049 ± 0.363

0.569 ± 0.075

3.157 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski