Opitutaceae bacterium EW11

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; unclassified Opitutaceae

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4994 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T6E196|A0A2T6E196_9BACT RNA-binding protein OS=Opitutaceae bacterium EW11 OX=2161865 GN=DB347_02485 PE=4 SV=1
MM1 pKa = 8.09AIPSDD6 pKa = 3.62RR7 pKa = 11.84VPQNVPGRR15 pKa = 11.84LYY17 pKa = 11.1VDD19 pKa = 4.26SSCIDD24 pKa = 3.44CDD26 pKa = 3.82LCRR29 pKa = 11.84EE30 pKa = 4.2TAPSLFARR38 pKa = 11.84FDD40 pKa = 3.52EE41 pKa = 4.93GGYY44 pKa = 10.15SYY46 pKa = 11.33VHH48 pKa = 6.25HH49 pKa = 6.62QPTTPEE55 pKa = 4.22EE56 pKa = 3.64IALAQEE62 pKa = 4.78AIEE65 pKa = 4.49GCPVEE70 pKa = 5.75AIGNDD75 pKa = 3.76GDD77 pKa = 5.22DD78 pKa = 3.94KK79 pKa = 11.63DD80 pKa = 3.85AAA82 pKa = 4.19

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6DVI1|A0A2T6DVI1_9BACT Type II secretion system F family protein OS=Opitutaceae bacterium EW11 OX=2161865 GN=DB347_13495 PE=3 SV=1
MM1 pKa = 7.15QPTFRR6 pKa = 11.84PHH8 pKa = 5.64RR9 pKa = 11.84KK10 pKa = 8.93KK11 pKa = 10.33RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 7.88IGFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.17GGRR28 pKa = 11.84KK29 pKa = 8.99VIASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.59GRR39 pKa = 11.84KK40 pKa = 8.95RR41 pKa = 11.84LTVVV45 pKa = 3.08

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4994

0

4994

1872195

27

2859

374.9

41.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.457 ± 0.049

0.941 ± 0.012

5.109 ± 0.028

5.71 ± 0.032

4.001 ± 0.022

8.01 ± 0.032

2.079 ± 0.017

4.431 ± 0.023

3.496 ± 0.033

10.335 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.821 ± 0.015

2.869 ± 0.031

5.552 ± 0.024

3.376 ± 0.023

7.437 ± 0.034

6.152 ± 0.032

5.47 ± 0.035

7.57 ± 0.026

1.639 ± 0.019

2.545 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski