Breznakibacter xylanolyticus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinilabiliaceae; Breznakibacter

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3475 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W7MTE1|A0A2W7MTE1_9BACT Uncharacterized protein OS=Breznakibacter xylanolyticus OX=990 GN=LX69_03182 PE=4 SV=1
VV1 pKa = 7.59DD2 pKa = 4.14FQGALTVSVTVSDD15 pKa = 4.6GKK17 pKa = 9.99ATSAPYY23 pKa = 8.45QTLVTVTPVNDD34 pKa = 3.49VPVVEE39 pKa = 4.38EE40 pKa = 3.58DD41 pKa = 3.17HH42 pKa = 7.13YY43 pKa = 10.94YY44 pKa = 10.0TGEE47 pKa = 3.99NTSVNMAVLANDD59 pKa = 3.86HH60 pKa = 7.07DD61 pKa = 4.58LADD64 pKa = 5.3GINGGINPASLQIVQSAGHH83 pKa = 6.83GICLVLANGNIVYY96 pKa = 8.95TPYY99 pKa = 10.46PGFSGTDD106 pKa = 2.98QFTYY110 pKa = 10.27RR111 pKa = 11.84VSDD114 pKa = 3.06VGFPMPALSGTAKK127 pKa = 10.37VVVDD131 pKa = 4.2VARR134 pKa = 11.84MSPVCVADD142 pKa = 3.55AASVLEE148 pKa = 4.56DD149 pKa = 3.82EE150 pKa = 5.22FVDD153 pKa = 3.9IHH155 pKa = 7.03VLTNDD160 pKa = 3.48SDD162 pKa = 3.89SGGDD166 pKa = 3.15IDD168 pKa = 6.27ASTLSVFSHH177 pKa = 7.52PIHH180 pKa = 6.82GNASVQPNGVVRR192 pKa = 11.84YY193 pKa = 8.97RR194 pKa = 11.84PNVNYY199 pKa = 10.31HH200 pKa = 6.1GEE202 pKa = 4.09DD203 pKa = 3.17LFSYY207 pKa = 7.31TVRR210 pKa = 11.84DD211 pKa = 3.52LTGLIGNVGTVRR223 pKa = 11.84VTVLPVNDD231 pKa = 4.26APVALTGQFTTRR243 pKa = 11.84EE244 pKa = 4.16AVAVTIPLGQLASDD258 pKa = 4.23PDD260 pKa = 4.03GDD262 pKa = 4.24IDD264 pKa = 3.39WSRR267 pKa = 11.84FKK269 pKa = 10.48IVKK272 pKa = 9.61LPSSGTVSVDD282 pKa = 3.1VKK284 pKa = 11.17ALTVVYY290 pKa = 9.86RR291 pKa = 11.84PNTAYY296 pKa = 10.61SGADD300 pKa = 3.01LFEE303 pKa = 4.15FTVVDD308 pKa = 3.37SHH310 pKa = 6.65GVQSNRR316 pKa = 11.84GSAAISVSNEE326 pKa = 3.38SPMVVDD332 pKa = 5.16DD333 pKa = 4.14VGEE336 pKa = 4.11VAEE339 pKa = 5.07DD340 pKa = 3.31SSIALDD346 pKa = 3.75VMSNDD351 pKa = 4.0SDD353 pKa = 4.07LQGDD357 pKa = 4.3LDD359 pKa = 4.25GASLMIIVPPAHH371 pKa = 7.05GIASVDD377 pKa = 3.51ARR379 pKa = 11.84SHH381 pKa = 5.64LVNYY385 pKa = 9.62YY386 pKa = 9.6PALNYY391 pKa = 10.72YY392 pKa = 10.29GVDD395 pKa = 3.08EE396 pKa = 5.47FSYY399 pKa = 9.81QVCDD403 pKa = 3.26NDD405 pKa = 5.06GYY407 pKa = 10.89CGRR410 pKa = 11.84ATVRR414 pKa = 11.84LTVHH418 pKa = 6.82PVNDD422 pKa = 3.99APLALDD428 pKa = 5.09DD429 pKa = 5.36FFEE432 pKa = 5.95LDD434 pKa = 3.5EE435 pKa = 5.29DD436 pKa = 3.99EE437 pKa = 5.01SLLVDD442 pKa = 3.99VLLNDD447 pKa = 4.01SDD449 pKa = 5.55GDD451 pKa = 4.01GQLDD455 pKa = 3.48AGSISVVRR463 pKa = 11.84QPAHH467 pKa = 5.42GQVVVDD473 pKa = 4.26AQHH476 pKa = 6.16GRR478 pKa = 11.84LLVVLDD484 pKa = 3.91NDD486 pKa = 4.06FFGDD490 pKa = 3.77DD491 pKa = 3.28EE492 pKa = 4.38LTYY495 pKa = 10.28RR496 pKa = 11.84VCDD499 pKa = 3.79DD500 pKa = 5.09RR501 pKa = 11.84GACSQAVAYY510 pKa = 10.24LKK512 pKa = 10.43VRR514 pKa = 11.84PVNDD518 pKa = 3.15APVAVNDD525 pKa = 4.04KK526 pKa = 10.95LVVEE530 pKa = 4.06TDD532 pKa = 3.03EE533 pKa = 4.99RR534 pKa = 11.84VVFTPLDD541 pKa = 3.74NDD543 pKa = 3.75SDD545 pKa = 4.15VDD547 pKa = 4.17DD548 pKa = 4.92GLNLSTLTLIVAPTWGKK565 pKa = 11.0AEE567 pKa = 3.99VLGNGQLSYY576 pKa = 11.12QSDD579 pKa = 3.38AGYY582 pKa = 10.81VGVVTLRR589 pKa = 11.84YY590 pKa = 9.12RR591 pKa = 11.84VCDD594 pKa = 3.52VGGLCAQADD603 pKa = 3.83VEE605 pKa = 4.58INVVSGNQPPVAVGDD620 pKa = 3.81LFSVNEE626 pKa = 4.45DD627 pKa = 2.62QDD629 pKa = 3.19MWLSVLDD636 pKa = 3.83NDD638 pKa = 4.9FDD640 pKa = 4.93SRR642 pKa = 11.84DD643 pKa = 3.71HH644 pKa = 6.9LLLSSLTVVEE654 pKa = 4.86APLHH658 pKa = 5.26GWATVDD664 pKa = 3.42ASAGKK669 pKa = 10.01IIYY672 pKa = 10.22RR673 pKa = 11.84PDD675 pKa = 3.23ADD677 pKa = 3.72YY678 pKa = 10.99WGVDD682 pKa = 3.11YY683 pKa = 11.15LRR685 pKa = 11.84YY686 pKa = 9.58KK687 pKa = 10.57VCDD690 pKa = 4.14DD691 pKa = 5.19NILPLCAEE699 pKa = 4.29ASVSISVMPVNDD711 pKa = 4.11APVGHH716 pKa = 7.4ADD718 pKa = 3.02HH719 pKa = 7.0YY720 pKa = 10.63EE721 pKa = 3.98AFDD724 pKa = 3.38VGEE727 pKa = 4.28YY728 pKa = 9.65NWNVLINDD736 pKa = 3.88TDD738 pKa = 3.64VDD740 pKa = 4.12DD741 pKa = 4.66TKK743 pKa = 11.21LSCRR747 pKa = 11.84LVDD750 pKa = 3.68ASGIAGEE757 pKa = 4.53VTLSAQGWLRR767 pKa = 11.84WRR769 pKa = 11.84PLPGCYY775 pKa = 9.74CSVQSLMYY783 pKa = 8.94EE784 pKa = 4.1VCDD787 pKa = 3.63PAGEE791 pKa = 4.18CDD793 pKa = 3.74VVQVLIDD800 pKa = 3.6VQYY803 pKa = 11.35SDD805 pKa = 3.86WDD807 pKa = 3.6GDD809 pKa = 4.34GIPDD813 pKa = 4.51AVDD816 pKa = 3.35GDD818 pKa = 4.16GDD820 pKa = 4.12SDD822 pKa = 4.97GDD824 pKa = 3.81GLPNYY829 pKa = 9.83RR830 pKa = 11.84DD831 pKa = 3.57ADD833 pKa = 3.85SDD835 pKa = 3.93GDD837 pKa = 4.47GIDD840 pKa = 3.61DD841 pKa = 4.34TIEE844 pKa = 4.21GGVADD849 pKa = 4.45VCTQFPVDD857 pKa = 3.76SDD859 pKa = 3.89GDD861 pKa = 4.13GVPDD865 pKa = 5.33FLDD868 pKa = 4.08ADD870 pKa = 4.07ADD872 pKa = 4.14GDD874 pKa = 4.3GVPDD878 pKa = 3.97AVEE881 pKa = 4.24LTDD884 pKa = 5.29DD885 pKa = 4.43CDD887 pKa = 4.64GDD889 pKa = 4.61GIPNHH894 pKa = 6.87LDD896 pKa = 3.47VVDD899 pKa = 4.54DD900 pKa = 3.69CTNRR904 pKa = 11.84VLVPDD909 pKa = 4.35TFTPNGDD916 pKa = 3.39GVNDD920 pKa = 3.66RR921 pKa = 11.84FVIPIVRR928 pKa = 11.84EE929 pKa = 3.75YY930 pKa = 10.24PINRR934 pKa = 11.84LTIFNRR940 pKa = 11.84WGNQVYY946 pKa = 9.58VADD949 pKa = 4.5NYY951 pKa = 11.14RR952 pKa = 11.84NDD954 pKa = 3.21WDD956 pKa = 4.13GRR958 pKa = 11.84SSTVTMGTNVLPEE971 pKa = 3.73GTYY974 pKa = 10.2FYY976 pKa = 11.4VLTLGVEE983 pKa = 4.14SRR985 pKa = 11.84LMKK988 pKa = 10.73GIVYY992 pKa = 10.09LKK994 pKa = 10.55KK995 pKa = 10.68

Molecular weight: 106.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W7NS53|A0A2W7NS53_9BACT Uncharacterized protein OS=Breznakibacter xylanolyticus OX=990 GN=LX69_01980 PE=4 SV=1
MM1 pKa = 7.47FYY3 pKa = 11.42SMFLICKK10 pKa = 9.64RR11 pKa = 11.84NRR13 pKa = 11.84NRR15 pKa = 11.84HH16 pKa = 4.94RR17 pKa = 11.84KK18 pKa = 8.82RR19 pKa = 11.84LRR21 pKa = 11.84KK22 pKa = 9.5DD23 pKa = 2.81RR24 pKa = 11.84CKK26 pKa = 10.93LLIPTVKK33 pKa = 10.42NCLLSQFYY41 pKa = 10.5AIALSCPSEE50 pKa = 3.97

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3475

0

3475

1239837

31

5336

356.8

40.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.368 ± 0.044

1.026 ± 0.017

5.54 ± 0.026

5.752 ± 0.045

4.618 ± 0.03

6.91 ± 0.044

2.323 ± 0.023

6.709 ± 0.042

5.791 ± 0.05

9.353 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.817 ± 0.025

5.176 ± 0.049

3.891 ± 0.03

3.903 ± 0.025

4.659 ± 0.038

6.358 ± 0.049

5.736 ± 0.055

6.998 ± 0.041

1.265 ± 0.016

3.805 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski