Acetonema longum DSM 6540

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Acetonema; Acetonema longum

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7NIE2|F7NIE2_9FIRM Uncharacterized protein OS=Acetonema longum DSM 6540 OX=1009370 GN=ALO_09224 PE=4 SV=1
MM1 pKa = 7.46GNVKK5 pKa = 10.29EE6 pKa = 4.3KK7 pKa = 10.7VAYY10 pKa = 10.01LQGLTKK16 pKa = 10.4GLNVSGEE23 pKa = 4.13SSEE26 pKa = 5.03GKK28 pKa = 9.95ILLNMIDD35 pKa = 3.75VLEE38 pKa = 4.42SLAQEE43 pKa = 3.97IHH45 pKa = 6.46LLSHH49 pKa = 7.07DD50 pKa = 3.78HH51 pKa = 7.16QEE53 pKa = 4.02LQDD56 pKa = 3.76YY57 pKa = 10.99VEE59 pKa = 5.11TIDD62 pKa = 5.3EE63 pKa = 4.57DD64 pKa = 3.87LHH66 pKa = 8.45DD67 pKa = 4.78LEE69 pKa = 4.45EE70 pKa = 4.31TVYY73 pKa = 10.77EE74 pKa = 4.69DD75 pKa = 4.09EE76 pKa = 5.06PADD79 pKa = 3.8EE80 pKa = 5.43PIVEE84 pKa = 4.39VEE86 pKa = 4.44CPRR89 pKa = 11.84CHH91 pKa = 5.55EE92 pKa = 4.38TVTFGADD99 pKa = 3.05NLEE102 pKa = 4.48DD103 pKa = 3.86EE104 pKa = 4.89DD105 pKa = 5.01VIEE108 pKa = 4.6ITCPTCGEE116 pKa = 4.06VVYY119 pKa = 10.65EE120 pKa = 4.02NTYY123 pKa = 9.34DD124 pKa = 3.55TDD126 pKa = 4.56ADD128 pKa = 3.78DD129 pKa = 5.71LIAVEE134 pKa = 4.56SKK136 pKa = 10.26PRR138 pKa = 11.84NINAGII144 pKa = 3.65

Molecular weight: 16.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7NQ96|F7NQ96_9FIRM Type II and III secretion system protein OS=Acetonema longum DSM 6540 OX=1009370 GN=ALO_21484 PE=3 SV=1
KKK2 pKa = 10.19KK3 pKa = 10.2VRR5 pKa = 11.84TRR7 pKa = 11.84ASVIVSRR14 pKa = 11.84GKKK17 pKa = 10.82MSGAAGRR24 pKa = 11.84AEEE27 pKa = 4.16RR28 pKa = 11.84QAAAWLGPRR37 pKa = 11.84GLMRR41 pKa = 11.84SRR43 pKa = 11.84AWGVLRR49 pKa = 11.84LSATPSPWQPVS

Molecular weight: 6.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4283

0

4283

1245218

30

4427

290.7

32.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.66 ± 0.045

1.167 ± 0.017

4.96 ± 0.028

6.047 ± 0.038

3.804 ± 0.025

7.776 ± 0.037

1.918 ± 0.018

6.965 ± 0.032

5.235 ± 0.032

10.059 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.021

3.616 ± 0.029

4.163 ± 0.027

4.146 ± 0.029

5.283 ± 0.034

5.571 ± 0.028

5.319 ± 0.031

7.31 ± 0.038

1.045 ± 0.017

3.185 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski