Luna mammarenavirus
Average proteome isoelectric point is 7.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U4VYZ4|A0A0U4VYZ4_9VIRU Pre-glycoprotein polyprotein GP complex OS=Luna mammarenavirus OX=883876 GN=GP PE=3 SV=1
MM1 pKa = 6.88 GQIVTFFQEE10 pKa = 4.01 VPHH13 pKa = 8.29 IIEE16 pKa = 3.78 EE17 pKa = 4.17 VMNIVLITLSLLAILKK33 pKa = 9.82 GVYY36 pKa = 9.96 NLATCGLLGLISFLFLCGRR55 pKa = 11.84 SCSITVKK62 pKa = 9.85 DD63 pKa = 3.69 TYY65 pKa = 9.48 EE66 pKa = 3.98 LRR68 pKa = 11.84 SVEE71 pKa = 4.79 LDD73 pKa = 3.15 MSTLNYY79 pKa = 9.69 TMPLSCSKK87 pKa = 10.84 NNSHH91 pKa = 7.33 HH92 pKa = 6.6 YY93 pKa = 9.98 IQTFNDD99 pKa = 3.26 TGLEE103 pKa = 4.01 LTLTNDD109 pKa = 2.91 SLLNHH114 pKa = 6.9 KK115 pKa = 9.74 FCNLSDD121 pKa = 3.19 AHH123 pKa = 6.64 KK124 pKa = 10.65 RR125 pKa = 11.84 NLYY128 pKa = 10.46 DD129 pKa = 3.31 HH130 pKa = 6.95 TLMSVITTFHH140 pKa = 6.68 LSIPNFNQYY149 pKa = 9.17 EE150 pKa = 4.06 AMACDD155 pKa = 4.1 FNGGKK160 pKa = 8.12 ITVQYY165 pKa = 9.95 NLSHH169 pKa = 6.86 SSPVDD174 pKa = 3.29 AANHH178 pKa = 6.36 CGTVANGVLDD188 pKa = 3.82 TFRR191 pKa = 11.84 RR192 pKa = 11.84 MHH194 pKa = 7.04 WGPAHH199 pKa = 6.6 FPDD202 pKa = 4.92 PPFQIGSVNCIQTSYY217 pKa = 10.99 KK218 pKa = 10.4 YY219 pKa = 10.93 LIIQNTTWEE228 pKa = 4.48 DD229 pKa = 3.31 HH230 pKa = 6.37 CQMSRR235 pKa = 11.84 PTPMGYY241 pKa = 10.6 LSLIAQRR248 pKa = 11.84 TRR250 pKa = 11.84 QLYY253 pKa = 9.02 ISRR256 pKa = 11.84 RR257 pKa = 11.84 LMGTFTWTLSDD268 pKa = 3.41 SAGNNLPGGYY278 pKa = 9.76 CLQRR282 pKa = 11.84 WMLIEE287 pKa = 6.12 AEE289 pKa = 4.5 MKK291 pKa = 10.75 CFGNTAVAKK300 pKa = 10.44 CNQQHH305 pKa = 6.86 DD306 pKa = 4.37 EE307 pKa = 4.55 EE308 pKa = 5.15 FCDD311 pKa = 3.62 MLRR314 pKa = 11.84 LFDD317 pKa = 4.54 FNKK320 pKa = 10.18 EE321 pKa = 3.9 AINRR325 pKa = 11.84 LRR327 pKa = 11.84 VEE329 pKa = 4.12 AEE331 pKa = 3.66 KK332 pKa = 10.9 SINLINKK339 pKa = 8.44 AVNSLINDD347 pKa = 3.3 QLIIRR352 pKa = 11.84 NHH354 pKa = 6.02 LRR356 pKa = 11.84 DD357 pKa = 3.78 LMGIPYY363 pKa = 10.31 CNYY366 pKa = 10.46 SKK368 pKa = 10.66 FWYY371 pKa = 10.77 LNDD374 pKa = 3.4 TRR376 pKa = 11.84 SGRR379 pKa = 11.84 TSLPKK384 pKa = 9.92 CWKK387 pKa = 9.79 VSNNSYY393 pKa = 10.95 LNEE396 pKa = 3.99 THH398 pKa = 7.01 FSDD401 pKa = 5.43 EE402 pKa = 4.38 IEE404 pKa = 4.17 EE405 pKa = 4.23 EE406 pKa = 4.26 ANNMITEE413 pKa = 4.45 MLRR416 pKa = 11.84 KK417 pKa = 9.39 EE418 pKa = 4.04 YY419 pKa = 10.44 EE420 pKa = 4.15 KK421 pKa = 10.87 RR422 pKa = 11.84 QSTTPLGLVDD432 pKa = 5.27 LFVFSTSFYY441 pKa = 10.09 LISIFLHH448 pKa = 6.43 LIKK451 pKa = 10.35 IPTHH455 pKa = 4.51 RR456 pKa = 11.84 HH457 pKa = 4.11 IVGKK461 pKa = 10.3 GCPKK465 pKa = 9.68 PHH467 pKa = 7.33 RR468 pKa = 11.84 LNHH471 pKa = 5.72 MAICSCGLYY480 pKa = 9.48 KK481 pKa = 10.54 QPGLPVRR488 pKa = 11.84 WKK490 pKa = 10.28 RR491 pKa = 3.49
Molecular weight: 56.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.978
IPC2_protein 7.102
IPC_protein 6.971
Toseland 6.795
ProMoST 7.629
Dawson 7.629
Bjellqvist 8.053
Wikipedia 7.541
Rodwell 7.644
Grimsley 6.883
Solomon 7.658
Lehninger 7.673
Nozaki 8.331
DTASelect 7.834
Thurlkill 7.878
EMBOSS 7.878
Sillero 8.185
Patrickios 3.668
IPC_peptide 7.658
IPC2_peptide 7.483
IPC2.peptide.svr19 7.171
Protein with the highest isoelectric point:
>tr|A0A0U4VYZ4|A0A0U4VYZ4_9VIRU Pre-glycoprotein polyprotein GP complex OS=Luna mammarenavirus OX=883876 GN=GP PE=3 SV=1
MM1 pKa = 7.74 GKK3 pKa = 8.68 NQSKK7 pKa = 10.74 SNRR10 pKa = 11.84 TPDD13 pKa = 3.71 NIEE16 pKa = 3.93 PRR18 pKa = 11.84 STLVPDD24 pKa = 4.45 ASGHH28 pKa = 5.26 GPEE31 pKa = 4.61 FCKK34 pKa = 10.86 SCWFEE39 pKa = 3.49 RR40 pKa = 11.84 RR41 pKa = 11.84 GLVKK45 pKa = 10.77 CHH47 pKa = 6.37 DD48 pKa = 4.86 HH49 pKa = 5.75 YY50 pKa = 12.07 LCMNCLTLLLTISDD64 pKa = 4.05 RR65 pKa = 11.84 CPICKK70 pKa = 10.12 YY71 pKa = 10.07 PLPTKK76 pKa = 10.36 LQLPKK81 pKa = 10.49 TPTAPHH87 pKa = 5.94 EE88 pKa = 4.49 TAHH91 pKa = 6.63 PPPPYY96 pKa = 10.53 SPP98 pKa = 4.25
Molecular weight: 11.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.714
IPC2_protein 7.732
IPC_protein 7.629
Toseland 7.161
ProMoST 8.244
Dawson 8.273
Bjellqvist 8.741
Wikipedia 8.185
Rodwell 8.287
Grimsley 7.161
Solomon 8.39
Lehninger 8.419
Nozaki 9.092
DTASelect 8.404
Thurlkill 8.448
EMBOSS 8.507
Sillero 8.829
Patrickios 3.897
IPC_peptide 8.39
IPC2_peptide 8.053
IPC2.peptide.svr19 7.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
589
98
491
294.5
33.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.735 ± 0.293
4.244 ± 1.261
4.075 ± 0.003
4.584 ± 0.219
4.244 ± 0.959
4.924 ± 0.366
4.244 ± 0.373
6.282 ± 1.402
4.924 ± 0.966
11.205 ± 0.436
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.226 ± 0.516
6.961 ± 1.253
5.772 ± 4.149
3.226 ± 0.516
4.924 ± 0.078
7.301 ± 0.069
7.131 ± 0.449
3.905 ± 0.811
1.358 ± 0.147
3.735 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here