Bat SARS-like coronavirus WIV1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Sarbecovirus; Severe acute respiratory syndrome-related coronavirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5WIP3|U5WIP3_SARS Uncharacterized protein OS=Bat SARS-like coronavirus WIV1 OX=1415852 PE=4 SV=1
MM1 pKa = 7.71NEE3 pKa = 3.36LTLIDD8 pKa = 5.36FYY10 pKa = 11.69LCFLAFLLFLVLIMLIIFWFSLEE33 pKa = 4.06LQDD36 pKa = 5.95IEE38 pKa = 4.55EE39 pKa = 4.77PCNKK43 pKa = 9.46VV44 pKa = 2.79

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5WI16|U5WI16_SARS Accessory protein 7a OS=Bat SARS-like coronavirus WIV1 OX=1415852 PE=4 SV=1
MM1 pKa = 7.29MPTTLFAGTHH11 pKa = 4.14ITMTTVYY18 pKa = 9.9RR19 pKa = 11.84ITVSQIQLSLLQVTAFQHH37 pKa = 5.26QNSKK41 pKa = 8.78KK42 pKa = 5.45TTKK45 pKa = 10.43LVVILRR51 pKa = 11.84IGTQVLKK58 pKa = 10.45TMSLYY63 pKa = 10.0MAISPKK69 pKa = 9.51FTTSLSLHH77 pKa = 6.65KK78 pKa = 10.58LLQTLVLKK86 pKa = 9.49MLHH89 pKa = 6.16SSSLTSLLKK98 pKa = 7.42THH100 pKa = 7.03RR101 pKa = 11.84MCKK104 pKa = 8.43YY105 pKa = 7.15TQSTALQEE113 pKa = 4.15LL114 pKa = 4.15

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

14265

44

7073

1097.3

122.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.115 ± 0.365

3.134 ± 0.415

5.216 ± 0.452

4.816 ± 0.445

4.809 ± 0.46

6.064 ± 0.52

2.061 ± 0.218

5.033 ± 0.57

5.622 ± 0.441

9.828 ± 0.743

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.419 ± 0.214

5.124 ± 0.347

4.059 ± 0.493

3.575 ± 0.597

3.694 ± 0.524

6.737 ± 0.292

7.242 ± 0.253

7.964 ± 0.757

1.087 ± 0.127

4.402 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski