Cebus albifrons polyomavirus 1
Average proteome isoelectric point is 5.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7QLH5|K7QLH5_9POLY Minor capsid protein OS=Cebus albifrons polyomavirus 1 OX=1236392 PE=3 SV=1
MM1 pKa = 7.12 GAVLAVLAEE10 pKa = 4.16 VFEE13 pKa = 4.57 LASVTGLSVDD23 pKa = 4.64 AILSGEE29 pKa = 4.2 AFTTAEE35 pKa = 4.35 LLQAHH40 pKa = 7.43 IANLVTVGGLTEE52 pKa = 4.49 AEE54 pKa = 3.83 ALAATEE60 pKa = 4.37 VTAEE64 pKa = 4.26 AYY66 pKa = 9.13 AALTSITSTFPQAFAALAATEE87 pKa = 4.1 LTATGSLTVGAAVAAALYY105 pKa = 8.99 PYY107 pKa = 9.42 TYY109 pKa = 11.04 DD110 pKa = 4.21 HH111 pKa = 6.41 STPLANLNTNMALQVWIPDD130 pKa = 3.65 LDD132 pKa = 4.16 LDD134 pKa = 4.32 FPGVRR139 pKa = 11.84 PFVRR143 pKa = 11.84 FVNYY147 pKa = 9.59 IDD149 pKa = 3.91 PAQWATDD156 pKa = 3.61 LFQAVGRR163 pKa = 11.84 YY164 pKa = 7.31 FWQTAQRR171 pKa = 11.84 YY172 pKa = 5.77 GQNLIEE178 pKa = 4.6 HH179 pKa = 6.88 EE180 pKa = 4.09 LRR182 pKa = 11.84 EE183 pKa = 4.28 TSRR186 pKa = 11.84 EE187 pKa = 4.0 LAVTAVTSVSDD198 pKa = 3.1 VLARR202 pKa = 11.84 YY203 pKa = 9.57 FEE205 pKa = 4.07 TARR208 pKa = 11.84 WAVSLLPRR216 pKa = 11.84 NIYY219 pKa = 10.11 QSLQNYY225 pKa = 6.9 YY226 pKa = 10.53 QEE228 pKa = 5.27 LPPLNPIQVRR238 pKa = 11.84 QLQRR242 pKa = 11.84 RR243 pKa = 11.84 IGGTIPDD250 pKa = 4.12 RR251 pKa = 11.84 LTFHH255 pKa = 7.14 DD256 pKa = 4.92 TIQTAEE262 pKa = 4.16 YY263 pKa = 9.86 VEE265 pKa = 4.43 KK266 pKa = 11.24 AEE268 pKa = 4.63 APGGANQRR276 pKa = 11.84 VAHH279 pKa = 6.83 DD280 pKa = 3.62 WLLPLLLGLYY290 pKa = 10.75 GDD292 pKa = 5.13 ISPSWEE298 pKa = 3.97 STLEE302 pKa = 4.04 DD303 pKa = 4.22 LEE305 pKa = 4.52 EE306 pKa = 5.02 EE307 pKa = 4.01 EE308 pKa = 6.13 DD309 pKa = 3.83 APQKK313 pKa = 10.67 KK314 pKa = 9.32 KK315 pKa = 10.48 RR316 pKa = 11.84 KK317 pKa = 8.98 KK318 pKa = 10.4 SKK320 pKa = 10.52
Molecular weight: 35.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.657
IPC2_protein 4.698
IPC_protein 4.609
Toseland 4.482
ProMoST 4.711
Dawson 4.558
Bjellqvist 4.698
Wikipedia 4.406
Rodwell 4.457
Grimsley 4.393
Solomon 4.558
Lehninger 4.507
Nozaki 4.66
DTASelect 4.787
Thurlkill 4.469
EMBOSS 4.431
Sillero 4.736
Patrickios 3.732
IPC_peptide 4.558
IPC2_peptide 4.724
IPC2.peptide.svr19 4.668
Protein with the highest isoelectric point:
>tr|K7QLH5|K7QLH5_9POLY Minor capsid protein OS=Cebus albifrons polyomavirus 1 OX=1236392 PE=3 SV=1
MM1 pKa = 7.89 DD2 pKa = 3.6 HH3 pKa = 6.91 TLTRR7 pKa = 11.84 DD8 pKa = 3.26 EE9 pKa = 5.01 SKK11 pKa = 11.4 LLMEE15 pKa = 5.29 LLNLPMEE22 pKa = 4.45 QYY24 pKa = 11.86 GNFPLMRR31 pKa = 11.84 KK32 pKa = 9.49 AFLQKK37 pKa = 10.55 CKK39 pKa = 10.03 ILHH42 pKa = 6.38 PDD44 pKa = 2.98 KK45 pKa = 11.56 GGDD48 pKa = 3.43 QEE50 pKa = 4.41 TAKK53 pKa = 10.37 MLISLYY59 pKa = 10.35 KK60 pKa = 9.96 RR61 pKa = 11.84 LEE63 pKa = 4.18 AEE65 pKa = 4.28 VQSLNTDD72 pKa = 3.4 DD73 pKa = 6.37 CFTTDD78 pKa = 3.41 QVCEE82 pKa = 3.83 ISNMIFLKK90 pKa = 10.43 DD91 pKa = 3.06 WRR93 pKa = 11.84 TCRR96 pKa = 11.84 MGSVKK101 pKa = 9.31 CTCLFCLLKK110 pKa = 10.49 RR111 pKa = 11.84 EE112 pKa = 4.74 HH113 pKa = 6.19 KK114 pKa = 9.7 QQLIGRR120 pKa = 11.84 PKK122 pKa = 9.67 VWGTCYY128 pKa = 10.5 CFNCYY133 pKa = 9.79 ILWFGLEE140 pKa = 3.73 HH141 pKa = 6.69 TYY143 pKa = 10.86 DD144 pKa = 4.34 IFLSWKK150 pKa = 9.6 ALIAITPFRR159 pKa = 11.84 NLNII163 pKa = 4.04
Molecular weight: 19.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.114
IPC2_protein 7.293
IPC_protein 7.176
Toseland 6.722
ProMoST 7.746
Dawson 7.863
Bjellqvist 8.317
Wikipedia 7.761
Rodwell 7.878
Grimsley 6.737
Solomon 7.922
Lehninger 7.951
Nozaki 8.682
DTASelect 8.009
Thurlkill 8.053
EMBOSS 8.083
Sillero 8.434
Patrickios 3.961
IPC_peptide 7.922
IPC2_peptide 7.585
IPC2.peptide.svr19 7.458
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1495
163
659
373.8
42.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.421 ± 2.057
2.408 ± 0.919
5.351 ± 0.424
6.756 ± 0.259
5.151 ± 0.594
5.217 ± 0.808
1.873 ± 0.366
4.95 ± 0.342
7.09 ± 1.69
11.037 ± 0.891
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.14 ± 0.483
4.415 ± 0.406
5.217 ± 1.132
4.615 ± 0.281
3.612 ± 0.569
5.619 ± 0.399
7.157 ± 0.528
5.953 ± 0.851
1.405 ± 0.239
3.612 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here