Listeria phage LWP01
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3B6XGQ7|A0A3B6XGQ7_9CAUD Uncharacterized protein OS=Listeria phage LWP01 OX=1633782 GN=UE46_p05210 PE=4 SV=1
MM1 pKa = 7.45 SGCEE5 pKa = 3.8 YY6 pKa = 10.5 CTDD9 pKa = 2.75 NWEE12 pKa = 4.06 ILTTFDD18 pKa = 4.19 EE19 pKa = 5.45 GPDD22 pKa = 3.47 VEE24 pKa = 5.08 GYY26 pKa = 9.89 VAIRR30 pKa = 11.84 DD31 pKa = 3.76 DD32 pKa = 4.39 SLVVSCDD39 pKa = 2.64 WGMTAVKK46 pKa = 10.2 MNFCPKK52 pKa = 10.11 CGAALKK58 pKa = 10.42 SRR60 pKa = 11.84 NDD62 pKa = 3.4 EE63 pKa = 4.32 EE64 pKa = 4.61 EE65 pKa = 4.58 PEE67 pKa = 4.13 LL68 pKa = 5.22
Molecular weight: 7.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.948
IPC_protein 3.846
Toseland 3.656
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.681
Grimsley 3.579
Solomon 3.808
Lehninger 3.757
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.961
Patrickios 0.769
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A3B6XH47|A0A3B6XH47_9CAUD HNHc domain-containing protein OS=Listeria phage LWP01 OX=1633782 GN=UE46_p05300 PE=4 SV=1
MM1 pKa = 7.64 SDD3 pKa = 3.49 VEE5 pKa = 4.53 VVRR8 pKa = 11.84 FFALLGFLLTVFAIFCYY25 pKa = 10.17 KK26 pKa = 10.32 VGYY29 pKa = 10.09 HH30 pKa = 6.69 DD31 pKa = 4.9 ARR33 pKa = 11.84 KK34 pKa = 9.8 KK35 pKa = 9.99 MKK37 pKa = 10.48 GGGQIEE43 pKa = 4.74 SIEE46 pKa = 4.28 KK47 pKa = 10.07 ARR49 pKa = 11.84 HH50 pKa = 4.38 EE51 pKa = 4.37 AATSRR56 pKa = 11.84 TSTHH60 pKa = 4.66 KK61 pKa = 9.58 TIPKK65 pKa = 9.91 HH66 pKa = 4.82 ILTRR70 pKa = 11.84 NGVGSKK76 pKa = 10.28
Molecular weight: 8.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.116
IPC2_protein 9.341
IPC_protein 9.341
Toseland 10.292
ProMoST 9.911
Dawson 10.409
Bjellqvist 10.014
Wikipedia 10.511
Rodwell 11.008
Grimsley 10.438
Solomon 10.438
Lehninger 10.423
Nozaki 10.277
DTASelect 9.999
Thurlkill 10.277
EMBOSS 10.657
Sillero 10.321
Patrickios 10.804
IPC_peptide 10.452
IPC2_peptide 8.565
IPC2.peptide.svr19 8.356
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
12824
67
1464
229.0
25.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.289 ± 0.487
0.671 ± 0.122
6.581 ± 0.267
8.32 ± 0.319
3.876 ± 0.28
6.535 ± 0.325
1.521 ± 0.177
6.098 ± 0.319
8.227 ± 0.432
8.718 ± 0.257
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.667 ± 0.125
4.593 ± 0.301
2.846 ± 0.343
3.649 ± 0.247
4.609 ± 0.355
5.536 ± 0.268
5.895 ± 0.369
6.597 ± 0.324
1.146 ± 0.126
3.626 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here