Amino acid dipepetide frequency for bacterium NHP-B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.873AlaAla: 6.873 ± 0.192
1.461AlaCys: 1.461 ± 0.063
3.575AlaAsp: 3.575 ± 0.111
4.116AlaGlu: 4.116 ± 0.116
4.465AlaPhe: 4.465 ± 0.123
5.35AlaGly: 5.35 ± 0.154
3.304AlaHis: 3.304 ± 0.111
3.711AlaIle: 3.711 ± 0.101
4.136AlaLys: 4.136 ± 0.114
11.539AlaLeu: 11.539 ± 0.229
2.454AlaMet: 2.454 ± 0.091
1.886AlaAsn: 1.886 ± 0.069
4.666AlaPro: 4.666 ± 0.129
4.247AlaGln: 4.247 ± 0.116
4.946AlaArg: 4.946 ± 0.146
6.384AlaSer: 6.384 ± 0.142
4.084AlaThr: 4.084 ± 0.109
4.876AlaVal: 4.876 ± 0.142
1.426AlaTrp: 1.426 ± 0.072
2.599AlaTyr: 2.599 ± 0.078
0.0AlaXaa: 0.0 ± 0.0
Cys
1.269CysAla: 1.269 ± 0.069
0.18CysCys: 0.18 ± 0.02
0.684CysAsp: 0.684 ± 0.045
0.713CysGlu: 0.713 ± 0.048
0.704CysPhe: 0.704 ± 0.042
0.981CysGly: 0.981 ± 0.055
0.437CysHis: 0.437 ± 0.041
0.509CysIle: 0.509 ± 0.039
0.428CysLys: 0.428 ± 0.032
1.843CysLeu: 1.843 ± 0.09
0.338CysMet: 0.338 ± 0.033
0.201CysAsn: 0.201 ± 0.025
0.664CysPro: 0.664 ± 0.046
0.547CysGln: 0.547 ± 0.042
0.6CysArg: 0.6 ± 0.045
0.626CysSer: 0.626 ± 0.045
0.611CysThr: 0.611 ± 0.043
1.467CysVal: 1.467 ± 0.068
0.198CysTrp: 0.198 ± 0.022
0.381CysTyr: 0.381 ± 0.032
0.0CysXaa: 0.0 ± 0.0
Asp
4.623AspAla: 4.623 ± 0.12
0.507AspCys: 0.507 ± 0.04
2.928AspAsp: 2.928 ± 0.111
2.925AspGlu: 2.925 ± 0.099
2.305AspPhe: 2.305 ± 0.08
3.231AspGly: 3.231 ± 0.106
1.799AspHis: 1.799 ± 0.075
3.316AspIle: 3.316 ± 0.109
2.576AspLys: 2.576 ± 0.093
5.1AspLeu: 5.1 ± 0.112
1.534AspMet: 1.534 ± 0.06
1.386AspAsn: 1.386 ± 0.085
2.792AspPro: 2.792 ± 0.101
2.032AspGln: 2.032 ± 0.074
2.608AspArg: 2.608 ± 0.095
2.052AspSer: 2.052 ± 0.095
2.943AspThr: 2.943 ± 0.085
4.64AspVal: 4.64 ± 0.13
0.565AspTrp: 0.565 ± 0.039
1.397AspTyr: 1.397 ± 0.072
0.0AspXaa: 0.0 ± 0.0
Glu
6.538GluAla: 6.538 ± 0.141
0.37GluCys: 0.37 ± 0.035
3.391GluAsp: 3.391 ± 0.116
4.186GluGlu: 4.186 ± 0.136
1.173GluPhe: 1.173 ± 0.062
4.919GluGly: 4.919 ± 0.126
1.607GluHis: 1.607 ± 0.069
2.468GluIle: 2.468 ± 0.085
4.78GluLys: 4.78 ± 0.136
3.77GluLeu: 3.77 ± 0.125
1.324GluMet: 1.324 ± 0.058
1.84GluAsn: 1.84 ± 0.079
2.192GluPro: 2.192 ± 0.079
2.029GluGln: 2.029 ± 0.089
3.35GluArg: 3.35 ± 0.114
2.984GluSer: 2.984 ± 0.102
3.158GluThr: 3.158 ± 0.097
3.461GluVal: 3.461 ± 0.116
0.448GluTrp: 0.448 ± 0.04
0.879GluTyr: 0.879 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.185PheAla: 3.185 ± 0.096
0.812PheCys: 0.812 ± 0.063
2.431PheAsp: 2.431 ± 0.093
2.434PheGlu: 2.434 ± 0.091
3.409PhePhe: 3.409 ± 0.124
2.751PheGly: 2.751 ± 0.1
1.322PheHis: 1.322 ± 0.064
1.988PheIle: 1.988 ± 0.083
1.636PheLys: 1.636 ± 0.08
6.288PheLeu: 6.288 ± 0.172
1.083PheMet: 1.083 ± 0.055
0.966PheAsn: 0.966 ± 0.052
1.915PhePro: 1.915 ± 0.082
1.662PheGln: 1.662 ± 0.073
1.831PheArg: 1.831 ± 0.069
3.813PheSer: 3.813 ± 0.13
2.026PheThr: 2.026 ± 0.084
3.735PheVal: 3.735 ± 0.116
0.897PheTrp: 0.897 ± 0.062
1.755PheTyr: 1.755 ± 0.071
0.0PheXaa: 0.0 ± 0.0
Gly
5.892GlyAla: 5.892 ± 0.161
0.984GlyCys: 0.984 ± 0.059
3.7GlyAsp: 3.7 ± 0.114
3.452GlyGlu: 3.452 ± 0.102
3.426GlyPhe: 3.426 ± 0.116
5.458GlyGly: 5.458 ± 0.191
2.3GlyHis: 2.3 ± 0.087
3.426GlyIle: 3.426 ± 0.117
3.694GlyLys: 3.694 ± 0.101
7.461GlyLeu: 7.461 ± 0.181
1.979GlyMet: 1.979 ± 0.083
1.534GlyAsn: 1.534 ± 0.065
2.824GlyPro: 2.824 ± 0.091
2.92GlyGln: 2.92 ± 0.099
4.052GlyArg: 4.052 ± 0.122
3.883GlySer: 3.883 ± 0.148
3.764GlyThr: 3.764 ± 0.125
5.569GlyVal: 5.569 ± 0.139
1.083GlyTrp: 1.083 ± 0.058
2.192GlyTyr: 2.192 ± 0.093
0.0GlyXaa: 0.0 ± 0.0
His
3.045HisAla: 3.045 ± 0.097
0.306HisCys: 0.306 ± 0.03
1.758HisAsp: 1.758 ± 0.082
1.613HisGlu: 1.613 ± 0.073
1.45HisPhe: 1.45 ± 0.068
2.078HisGly: 2.078 ± 0.087
1.595HisHis: 1.595 ± 0.079
2.105HisIle: 2.105 ± 0.082
1.901HisLys: 1.901 ± 0.073
3.554HisLeu: 3.554 ± 0.108
0.929HisMet: 0.929 ± 0.054
1.144HisAsn: 1.144 ± 0.059
1.939HisPro: 1.939 ± 0.083
1.552HisGln: 1.552 ± 0.07
1.528HisArg: 1.528 ± 0.067
1.275HisSer: 1.275 ± 0.061
2.483HisThr: 2.483 ± 0.106
2.754HisVal: 2.754 ± 0.096
0.335HisTrp: 0.335 ± 0.033
0.952HisTyr: 0.952 ± 0.053
0.0HisXaa: 0.0 ± 0.0
Ile
3.953IleAla: 3.953 ± 0.115
0.556IleCys: 0.556 ± 0.041
2.867IleAsp: 2.867 ± 0.098
2.786IleGlu: 2.786 ± 0.087
2.148IlePhe: 2.148 ± 0.1
2.899IleGly: 2.899 ± 0.112
1.645IleHis: 1.645 ± 0.076
2.096IleIle: 2.096 ± 0.087
2.681IleLys: 2.681 ± 0.097
4.788IleLeu: 4.788 ± 0.129
1.045IleMet: 1.045 ± 0.057
1.505IleAsn: 1.505 ± 0.071
2.343IlePro: 2.343 ± 0.088
2.308IleGln: 2.308 ± 0.083
2.288IleArg: 2.288 ± 0.094
3.059IleSer: 3.059 ± 0.085
3.158IleThr: 3.158 ± 0.099
3.298IleVal: 3.298 ± 0.099
0.422IleTrp: 0.422 ± 0.035
1.17IleTyr: 1.17 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
5.7LysAla: 5.7 ± 0.143
0.247LysCys: 0.247 ± 0.029
3.362LysAsp: 3.362 ± 0.102
4.299LysGlu: 4.299 ± 0.126
0.85LysPhe: 0.85 ± 0.051
4.075LysGly: 4.075 ± 0.12
1.581LysHis: 1.581 ± 0.065
2.891LysIle: 2.891 ± 0.099
5.557LysLys: 5.557 ± 0.176
3.636LysLeu: 3.636 ± 0.095
1.458LysMet: 1.458 ± 0.075
2.402LysAsn: 2.402 ± 0.086
2.655LysPro: 2.655 ± 0.095
2.122LysGln: 2.122 ± 0.077
3.825LysArg: 3.825 ± 0.125
2.838LysSer: 2.838 ± 0.087
3.877LysThr: 3.877 ± 0.121
2.832LysVal: 2.832 ± 0.088
0.402LysTrp: 0.402 ± 0.034
0.76LysTyr: 0.76 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
9.484LeuAla: 9.484 ± 0.181
1.843LeuCys: 1.843 ± 0.084
5.531LeuAsp: 5.531 ± 0.124
5.909LeuGlu: 5.909 ± 0.115
5.435LeuPhe: 5.435 ± 0.173
7.667LeuGly: 7.667 ± 0.174
2.928LeuHis: 2.928 ± 0.104
4.608LeuIle: 4.608 ± 0.117
6.139LeuLys: 6.139 ± 0.142
11.05LeuLeu: 11.05 ± 0.248
2.969LeuMet: 2.969 ± 0.093
2.856LeuAsn: 2.856 ± 0.107
5.528LeuPro: 5.528 ± 0.145
3.409LeuGln: 3.409 ± 0.088
5.604LeuArg: 5.604 ± 0.134
9.787LeuSer: 9.787 ± 0.206
6.858LeuThr: 6.858 ± 0.159
6.646LeuVal: 6.646 ± 0.17
1.953LeuTrp: 1.953 ± 0.096
2.853LeuTyr: 2.853 ± 0.108
0.0LeuXaa: 0.0 ± 0.0
Met
2.696MetAla: 2.696 ± 0.086
0.376MetCys: 0.376 ± 0.034
1.258MetAsp: 1.258 ± 0.061
1.103MetGlu: 1.103 ± 0.054
0.885MetPhe: 0.885 ± 0.055
2.113MetGly: 2.113 ± 0.082
0.835MetHis: 0.835 ± 0.051
1.354MetIle: 1.354 ± 0.069
1.584MetLys: 1.584 ± 0.079
2.352MetLeu: 2.352 ± 0.09
0.876MetMet: 0.876 ± 0.051
0.929MetAsn: 0.929 ± 0.049
1.578MetPro: 1.578 ± 0.071
0.908MetGln: 0.908 ± 0.049
1.653MetArg: 1.653 ± 0.07
1.985MetSer: 1.985 ± 0.072
1.72MetThr: 1.72 ± 0.072
1.784MetVal: 1.784 ± 0.083
0.329MetTrp: 0.329 ± 0.033
0.486MetTyr: 0.486 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
2.48AsnAla: 2.48 ± 0.09
0.204AsnCys: 0.204 ± 0.027
1.144AsnAsp: 1.144 ± 0.071
1.179AsnGlu: 1.179 ± 0.058
0.94AsnPhe: 0.94 ± 0.056
1.444AsnGly: 1.444 ± 0.086
0.882AsnHis: 0.882 ± 0.054
1.767AsnIle: 1.767 ± 0.081
1.554AsnLys: 1.554 ± 0.062
2.911AsnLeu: 2.911 ± 0.096
0.812AsnMet: 0.812 ± 0.052
0.943AsnAsn: 0.943 ± 0.064
1.91AsnPro: 1.91 ± 0.075
1.202AsnGln: 1.202 ± 0.065
1.371AsnArg: 1.371 ± 0.069
1.045AsnSer: 1.045 ± 0.059
1.764AsnThr: 1.764 ± 0.071
2.137AsnVal: 2.137 ± 0.088
0.242AsnTrp: 0.242 ± 0.03
0.629AsnTyr: 0.629 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.88ProAla: 3.88 ± 0.119
0.771ProCys: 0.771 ± 0.043
2.614ProAsp: 2.614 ± 0.1
3.173ProGlu: 3.173 ± 0.103
2.859ProPhe: 2.859 ± 0.088
2.992ProGly: 2.992 ± 0.088
2.239ProHis: 2.239 ± 0.081
1.851ProIle: 1.851 ± 0.072
2.282ProLys: 2.282 ± 0.088
6.497ProLeu: 6.497 ± 0.156
1.269ProMet: 1.269 ± 0.058
0.981ProAsn: 0.981 ± 0.06
3.115ProPro: 3.115 ± 0.136
2.716ProGln: 2.716 ± 0.088
2.215ProArg: 2.215 ± 0.078
4.41ProSer: 4.41 ± 0.136
2.713ProThr: 2.713 ± 0.096
3.284ProVal: 3.284 ± 0.097
0.83ProTrp: 0.83 ± 0.053
1.668ProTyr: 1.668 ± 0.07
0.0ProXaa: 0.0 ± 0.0
Gln
4.087GlnAla: 4.087 ± 0.13
0.422GlnCys: 0.422 ± 0.032
2.728GlnAsp: 2.728 ± 0.096
3.42GlnGlu: 3.42 ± 0.107
1.03GlnPhe: 1.03 ± 0.055
3.662GlnGly: 3.662 ± 0.105
1.429GlnHis: 1.429 ± 0.08
1.493GlnIle: 1.493 ± 0.07
3.886GlnLys: 3.886 ± 0.121
2.675GlnLeu: 2.675 ± 0.087
0.929GlnMet: 0.929 ± 0.049
1.423GlnAsn: 1.423 ± 0.076
1.787GlnPro: 1.787 ± 0.089
1.991GlnGln: 1.991 ± 0.094
2.626GlnArg: 2.626 ± 0.103
2.37GlnSer: 2.37 ± 0.1
2.597GlnThr: 2.597 ± 0.09
2.265GlnVal: 2.265 ± 0.078
0.474GlnTrp: 0.474 ± 0.039
0.734GlnTyr: 0.734 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
4.559ArgAla: 4.559 ± 0.134
0.603ArgCys: 0.603 ± 0.04
2.687ArgAsp: 2.687 ± 0.098
2.92ArgGlu: 2.92 ± 0.112
2.908ArgPhe: 2.908 ± 0.098
3.519ArgGly: 3.519 ± 0.11
2.023ArgHis: 2.023 ± 0.079
2.701ArgIle: 2.701 ± 0.097
2.579ArgLys: 2.579 ± 0.093
6.713ArgLeu: 6.713 ± 0.134
1.531ArgMet: 1.531 ± 0.073
1.29ArgAsn: 1.29 ± 0.059
2.602ArgPro: 2.602 ± 0.1
2.404ArgGln: 2.404 ± 0.1
3.234ArgArg: 3.234 ± 0.129
2.815ArgSer: 2.815 ± 0.105
2.669ArgThr: 2.669 ± 0.088
4.081ArgVal: 4.081 ± 0.126
0.652ArgTrp: 0.652 ± 0.043
1.758ArgTyr: 1.758 ± 0.073
0.0ArgXaa: 0.0 ± 0.0
Ser
4.899SerAla: 4.899 ± 0.119
1.039SerCys: 1.039 ± 0.058
2.765SerAsp: 2.765 ± 0.098
3.077SerGlu: 3.077 ± 0.114
3.962SerPhe: 3.962 ± 0.105
4.349SerGly: 4.349 ± 0.143
2.285SerHis: 2.285 ± 0.088
2.87SerIle: 2.87 ± 0.091
2.448SerLys: 2.448 ± 0.087
8.829SerLeu: 8.829 ± 0.187
1.84SerMet: 1.84 ± 0.083
1.223SerAsn: 1.223 ± 0.057
4.008SerPro: 4.008 ± 0.139
2.873SerGln: 2.873 ± 0.1
3.033SerArg: 3.033 ± 0.093
5.115SerSer: 5.115 ± 0.183
3.08SerThr: 3.08 ± 0.1
4.494SerVal: 4.494 ± 0.126
1.065SerTrp: 1.065 ± 0.063
1.895SerTyr: 1.895 ± 0.079
0.0SerXaa: 0.0 ± 0.0
Thr
3.892ThrAla: 3.892 ± 0.102
0.821ThrCys: 0.821 ± 0.053
2.3ThrAsp: 2.3 ± 0.086
2.189ThrGlu: 2.189 ± 0.094
3.027ThrPhe: 3.027 ± 0.102
3.438ThrGly: 3.438 ± 0.098
2.53ThrHis: 2.53 ± 0.082
2.448ThrIle: 2.448 ± 0.088
2.599ThrLys: 2.599 ± 0.097
8.133ThrLeu: 8.133 ± 0.154
1.246ThrMet: 1.246 ± 0.065
1.234ThrAsn: 1.234 ± 0.069
4.299ThrPro: 4.299 ± 0.117
2.736ThrGln: 2.736 ± 0.085
2.867ThrArg: 2.867 ± 0.101
3.956ThrSer: 3.956 ± 0.12
3.094ThrThr: 3.094 ± 0.115
3.074ThrVal: 3.074 ± 0.1
0.879ThrTrp: 0.879 ± 0.053
1.828ThrTyr: 1.828 ± 0.069
0.0ThrXaa: 0.0 ± 0.0
Val
5.432ValAla: 5.432 ± 0.133
1.295ValCys: 1.295 ± 0.065
3.313ValAsp: 3.313 ± 0.105
3.231ValGlu: 3.231 ± 0.11
3.362ValPhe: 3.362 ± 0.123
5.161ValGly: 5.161 ± 0.145
2.049ValHis: 2.049 ± 0.078
3.397ValIle: 3.397 ± 0.107
3.316ValLys: 3.316 ± 0.116
7.778ValLeu: 7.778 ± 0.19
2.078ValMet: 2.078 ± 0.072
1.878ValAsn: 1.878 ± 0.076
3.467ValPro: 3.467 ± 0.105
2.131ValGln: 2.131 ± 0.095
4.069ValArg: 4.069 ± 0.106
4.966ValSer: 4.966 ± 0.129
3.88ValThr: 3.88 ± 0.095
5.377ValVal: 5.377 ± 0.142
1.086ValTrp: 1.086 ± 0.057
1.758ValTyr: 1.758 ± 0.069
0.0ValXaa: 0.0 ± 0.0
Trp
1.097TrpAla: 1.097 ± 0.058
0.262TrpCys: 0.262 ± 0.029
0.559TrpAsp: 0.559 ± 0.039
0.652TrpGlu: 0.652 ± 0.043
0.507TrpPhe: 0.507 ± 0.041
1.228TrpGly: 1.228 ± 0.065
0.524TrpHis: 0.524 ± 0.041
0.716TrpIle: 0.716 ± 0.053
0.457TrpLys: 0.457 ± 0.039
1.453TrpLeu: 1.453 ± 0.068
0.509TrpMet: 0.509 ± 0.037
0.297TrpAsn: 0.297 ± 0.029
0.751TrpPro: 0.751 ± 0.053
0.824TrpGln: 0.824 ± 0.058
0.955TrpArg: 0.955 ± 0.062
0.803TrpSer: 0.803 ± 0.049
0.611TrpThr: 0.611 ± 0.043
1.077TrpVal: 1.077 ± 0.058
0.21TrpTrp: 0.21 ± 0.025
0.274TrpTyr: 0.274 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.518TyrAla: 2.518 ± 0.091
0.323TyrCys: 0.323 ± 0.029
1.665TyrAsp: 1.665 ± 0.068
1.49TyrGlu: 1.49 ± 0.062
1.092TyrPhe: 1.092 ± 0.057
2.273TyrGly: 2.273 ± 0.087
0.981TyrHis: 0.981 ± 0.05
1.301TyrIle: 1.301 ± 0.064
1.135TyrLys: 1.135 ± 0.063
2.69TyrLeu: 2.69 ± 0.099
0.6TyrMet: 0.6 ± 0.044
0.643TyrAsn: 0.643 ± 0.047
1.324TyrPro: 1.324 ± 0.061
1.415TyrGln: 1.415 ± 0.06
1.592TyrArg: 1.592 ± 0.072
0.981TyrSer: 0.981 ± 0.065
1.499TyrThr: 1.499 ± 0.072
2.174TyrVal: 2.174 ± 0.081
0.271TyrTrp: 0.271 ± 0.03
0.629TyrTyr: 0.629 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1115 proteins (343533 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski