Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 M:B

Average proteome isoelectric point is 7.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P69723|VIF_HV1H2 Virion infectivity factor OS=Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) OX=11706 GN=vif PE=1 SV=1
QQ1 pKa = 7.83PIPIVAIVALVVAIIIAIVVWSIVIIEE28 pKa = 3.96YY29 pKa = 10.52RR30 pKa = 11.84KK31 pKa = 9.52ILRR34 pKa = 11.84QRR36 pKa = 11.84KK37 pKa = 8.02IDD39 pKa = 3.69RR40 pKa = 11.84LIDD43 pKa = 3.54RR44 pKa = 11.84LIEE47 pKa = 4.08RR48 pKa = 11.84AEE50 pKa = 4.06DD51 pKa = 3.39SGNEE55 pKa = 4.05SEE57 pKa = 5.18GEE59 pKa = 3.65ISALVEE65 pKa = 3.91MGVEE69 pKa = 4.38MGHH72 pKa = 6.17HH73 pKa = 6.54APWDD77 pKa = 3.7VDD79 pKa = 3.84DD80 pKa = 4.78LL81 pKa = 4.6

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P04608-2|TAT-2_HV1H2 Isoform of P04608 Isoform Short of Protein Tat OS=Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) OX=11706 GN=tat PE=1 SV=2
MM1 pKa = 7.46EE2 pKa = 5.27PVDD5 pKa = 4.56PRR7 pKa = 11.84LEE9 pKa = 4.15PWKK12 pKa = 10.86HH13 pKa = 6.31PGSQPKK19 pKa = 8.02TACTNCYY26 pKa = 9.77CKK28 pKa = 10.32KK29 pKa = 10.48CCFHH33 pKa = 6.68CQVCFITKK41 pKa = 10.28ALGISYY47 pKa = 9.84GRR49 pKa = 11.84KK50 pKa = 9.0KK51 pKa = 10.12RR52 pKa = 11.84RR53 pKa = 11.84QRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84AHH59 pKa = 5.99QNSQTHH65 pKa = 4.76QASLSKK71 pKa = 10.68QQ72 pKa = 3.15

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

3640

72

1435

364.0

41.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.291 ± 0.401

2.06 ± 0.373

3.764 ± 0.345

7.033 ± 0.472

2.418 ± 0.243

7.17 ± 0.227

2.5 ± 0.493

6.621 ± 0.612

6.841 ± 0.996

8.104 ± 0.486

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.115 ± 0.177

4.505 ± 0.667

5.357 ± 0.593

5.934 ± 0.511

6.346 ± 0.972

5.44 ± 0.631

6.071 ± 0.302

6.126 ± 0.43

2.747 ± 0.314

2.555 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski