Succinatimonas sp. CAG:777
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1970 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R5ELA1|R5ELA1_9GAMM Protein TolA OS=Succinatimonas sp. CAG:777 OX=1262974 GN=BN779_00803 PE=4 SV=1
MM1 pKa = 7.19 FVINNEE7 pKa = 3.69 ICSQCGEE14 pKa = 4.35 CQDD17 pKa = 3.86 ICPCGAIEE25 pKa = 4.22 QQPDD29 pKa = 3.26 DD30 pKa = 4.12 SFKK33 pKa = 10.56 IDD35 pKa = 4.7 AEE37 pKa = 4.31 MCSDD41 pKa = 4.28 CAACQSVCPFEE52 pKa = 6.65 AIEE55 pKa = 4.16 EE56 pKa = 4.33 KK57 pKa = 10.71 EE58 pKa = 3.9 DD59 pKa = 3.96 DD60 pKa = 3.89 KK61 pKa = 12.07
Molecular weight: 6.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.932
IPC2_protein 3.643
IPC_protein 3.541
Toseland 3.363
ProMoST 3.732
Dawson 3.541
Bjellqvist 3.706
Wikipedia 3.49
Rodwell 3.389
Grimsley 3.287
Solomon 3.503
Lehninger 3.452
Nozaki 3.681
DTASelect 3.821
Thurlkill 3.439
EMBOSS 3.49
Sillero 3.668
Patrickios 0.006
IPC_peptide 3.49
IPC2_peptide 3.643
IPC2.peptide.svr19 3.678
Protein with the highest isoelectric point:
>tr|R5EUU8|R5EUU8_9GAMM Ankyrin OS=Succinatimonas sp. CAG:777 OX=1262974 GN=BN779_00644 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.52 RR3 pKa = 11.84 TFQPNVTKK11 pKa = 10.67 RR12 pKa = 11.84 KK13 pKa = 7.14 RR14 pKa = 11.84 THH16 pKa = 5.69 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MRR23 pKa = 11.84 TADD26 pKa = 3.23 GRR28 pKa = 11.84 KK29 pKa = 8.25 VLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.95 GRR39 pKa = 11.84 KK40 pKa = 9.09 RR41 pKa = 11.84 LTTVNGG47 pKa = 3.51
Molecular weight: 5.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.442
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1970
0
1970
633173
29
1745
321.4
35.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.56 ± 0.063
1.43 ± 0.022
6.121 ± 0.041
6.175 ± 0.045
4.548 ± 0.046
6.256 ± 0.059
1.788 ± 0.022
7.044 ± 0.048
7.659 ± 0.049
9.745 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.4 ± 0.025
5.168 ± 0.046
3.404 ± 0.031
3.078 ± 0.033
3.936 ± 0.035
7.063 ± 0.056
5.323 ± 0.036
7.004 ± 0.049
0.708 ± 0.015
3.59 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here