Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas knackmussii

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5616 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024HG58|A0A024HG58_PSEKB Uncharacterized protein OS=Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13) OX=1301098 GN=PKB_2140 PE=4 SV=1
MM1 pKa = 7.37FRR3 pKa = 11.84LTPISAALLLLAAGQAMADD22 pKa = 3.78DD23 pKa = 4.36SSANQVQNGEE33 pKa = 4.05QNIANVSQKK42 pKa = 9.46QASWSTANQTQTGTGNDD59 pKa = 3.13AMAVQQEE66 pKa = 4.58STGSIVQSLSGDD78 pKa = 3.15HH79 pKa = 5.93NAAYY83 pKa = 10.34GEE85 pKa = 4.11QNYY88 pKa = 10.38GEE90 pKa = 4.5INSSINQQGNGDD102 pKa = 3.72WNDD105 pKa = 3.4NFASQYY111 pKa = 10.45YY112 pKa = 10.56GSDD115 pKa = 3.26NQALQIQDD123 pKa = 3.37GSRR126 pKa = 11.84SFSMVYY132 pKa = 9.68QEE134 pKa = 4.66GQVGSQGTTQQSGGNHH150 pKa = 5.71EE151 pKa = 4.95ANIEE155 pKa = 4.01QIGFGEE161 pKa = 4.55GNRR164 pKa = 11.84ALIIQSGDD172 pKa = 3.17ANRR175 pKa = 11.84GSIKK179 pKa = 10.53QSDD182 pKa = 4.35QISGHH187 pKa = 4.93QEE189 pKa = 3.71VYY191 pKa = 10.25QQGSSNTANGWQNLGLGGTVLFNQAGDD218 pKa = 3.54NNYY221 pKa = 10.7AEE223 pKa = 4.67VWQDD227 pKa = 3.45SQDD230 pKa = 3.33SSQASIAQFGSTNEE244 pKa = 3.86AVIDD248 pKa = 3.84QSFGMAMDD256 pKa = 4.91PYY258 pKa = 11.13GVGNVASINQSGDD271 pKa = 3.17RR272 pKa = 11.84NAAWADD278 pKa = 3.47QYY280 pKa = 11.4EE281 pKa = 4.83SKK283 pKa = 11.02GSNTALYY290 pKa = 10.74QNGTDD295 pKa = 4.02NLQLTYY301 pKa = 9.66QTGSGHH307 pKa = 5.71SLVVTSNGTGNKK319 pKa = 9.52VYY321 pKa = 10.47MSDD324 pKa = 2.79WQGARR329 pKa = 11.84RR330 pKa = 11.84GGQSGQGNNAAIAQNGGGNVAQVKK354 pKa = 9.1QQDD357 pKa = 4.04TGNKK361 pKa = 7.52TQLTQNGTNNDD372 pKa = 3.8LNVSQAEE379 pKa = 4.64SYY381 pKa = 10.96NEE383 pKa = 4.57LYY385 pKa = 10.74FEE387 pKa = 4.99QNGSDD392 pKa = 3.89NILVADD398 pKa = 3.71QRR400 pKa = 11.84GTGNYY405 pKa = 7.6ATGTSNGDD413 pKa = 3.48FNKK416 pKa = 9.82VEE418 pKa = 4.51LDD420 pKa = 3.23QSGAGNQAYY429 pKa = 10.32VNQLYY434 pKa = 10.65GDD436 pKa = 4.31HH437 pKa = 6.74NLASVTQTDD446 pKa = 4.57GVNLAYY452 pKa = 8.92ITQGGTANQATVSQAGVNMNANVQQFGTGNQSTITQQQ489 pKa = 2.8

Molecular weight: 51.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024HQV8|A0A024HQV8_PSEKB Exodeoxyribonuclease III OS=Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13) OX=1301098 GN=crc PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.31RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5616

0

5616

1780795

29

3787

317.1

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.552 ± 0.042

1.022 ± 0.011

5.351 ± 0.025

5.954 ± 0.036

3.573 ± 0.019

8.226 ± 0.031

2.269 ± 0.016

4.356 ± 0.023

3.196 ± 0.029

12.104 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.014

2.77 ± 0.021

5.034 ± 0.026

4.429 ± 0.026

6.985 ± 0.034

5.557 ± 0.025

4.435 ± 0.03

6.989 ± 0.028

1.451 ± 0.013

2.528 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski