Blackberry chlorotic ringspot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2QF35|Q2QF35_9BROM ATP-dependent helicase OS=Blackberry chlorotic ringspot virus OX=339420 PE=2 SV=1
MM1 pKa = 8.11DD2 pKa = 3.91YY3 pKa = 11.08VKK5 pKa = 10.83EE6 pKa = 3.89NLIVYY11 pKa = 7.5SLRR14 pKa = 11.84SRR16 pKa = 11.84LEE18 pKa = 4.16FIQSFGVEE26 pKa = 3.42PDD28 pKa = 3.39MYY30 pKa = 10.74KK31 pKa = 10.34EE32 pKa = 4.13WVSMFLFKK40 pKa = 10.75FIVEE44 pKa = 4.27HH45 pKa = 4.93TAKK48 pKa = 10.4FDD50 pKa = 3.67FATIDD55 pKa = 3.63CTFGMIVARR64 pKa = 11.84LIPGYY69 pKa = 10.58SDD71 pKa = 5.81LDD73 pKa = 4.35DD74 pKa = 4.53EE75 pKa = 5.62DD76 pKa = 5.39EE77 pKa = 4.18EE78 pKa = 4.41HH79 pKa = 6.85SPRR82 pKa = 11.84EE83 pKa = 3.52IDD85 pKa = 4.78SFYY88 pKa = 11.21LPYY91 pKa = 10.73DD92 pKa = 4.33DD93 pKa = 6.51LDD95 pKa = 3.56VDD97 pKa = 4.14YY98 pKa = 8.4TTIDD102 pKa = 3.37YY103 pKa = 10.85RR104 pKa = 11.84RR105 pKa = 11.84DD106 pKa = 3.64DD107 pKa = 3.82VEE109 pKa = 4.39VPTNEE114 pKa = 3.85VLNLVSNASYY124 pKa = 10.9VPEE127 pKa = 4.34GVSWGDD133 pKa = 3.3VTDD136 pKa = 4.73SSFEE140 pKa = 3.9DD141 pKa = 3.41HH142 pKa = 7.15LEE144 pKa = 4.45EE145 pKa = 4.5IQEE148 pKa = 4.21LPTFNVDD155 pKa = 3.47EE156 pKa = 4.58PVVSSSEE163 pKa = 3.89AGLPDD168 pKa = 5.52DD169 pKa = 5.92DD170 pKa = 4.87GTIQDD175 pKa = 4.53VVWVDD180 pKa = 3.28QSDD183 pKa = 4.55PIVSDD188 pKa = 3.71VVADD192 pKa = 3.83ADD194 pKa = 3.97LRR196 pKa = 11.84NCGFVDD202 pKa = 3.81YY203 pKa = 10.84QSGSIKK209 pKa = 9.22TSKK212 pKa = 9.41WKK214 pKa = 10.21PKK216 pKa = 9.81VIQVRR221 pKa = 11.84PDD223 pKa = 3.12PSIIQNAVDD232 pKa = 4.22EE233 pKa = 4.74IFPNHH238 pKa = 6.04HH239 pKa = 6.65HH240 pKa = 6.8VDD242 pKa = 3.39DD243 pKa = 4.89KK244 pKa = 11.42FFQEE248 pKa = 4.07WVEE251 pKa = 3.92THH253 pKa = 7.34DD254 pKa = 4.63IDD256 pKa = 6.25LEE258 pKa = 4.44VTNCDD263 pKa = 3.15LDD265 pKa = 3.52MSVFNDD271 pKa = 3.25WTKK274 pKa = 11.19GSDD277 pKa = 3.62SRR279 pKa = 11.84LMPAFSVGGLSHH291 pKa = 6.8TVPTQRR297 pKa = 11.84EE298 pKa = 3.73TLLAVKK304 pKa = 9.36KK305 pKa = 10.66RR306 pKa = 11.84NMNVPEE312 pKa = 4.35LQSQFDD318 pKa = 4.09HH319 pKa = 6.84NDD321 pKa = 2.97VLNRR325 pKa = 11.84CVNRR329 pKa = 11.84FLTHH333 pKa = 6.03VVDD336 pKa = 3.81KK337 pKa = 10.85TRR339 pKa = 11.84LSKK342 pKa = 10.82LMPISGEE349 pKa = 3.87EE350 pKa = 3.55VHH352 pKa = 6.39YY353 pKa = 10.45FNQYY357 pKa = 9.67VEE359 pKa = 4.57NKK361 pKa = 9.71NPPLSEE367 pKa = 4.12YY368 pKa = 10.28KK369 pKa = 10.94GPIPLVALDD378 pKa = 5.02KK379 pKa = 10.39YY380 pKa = 9.66MHH382 pKa = 6.57MIKK385 pKa = 7.75TTLKK389 pKa = 10.13PVEE392 pKa = 4.31EE393 pKa = 5.13DD394 pKa = 3.26SLHH397 pKa = 5.79VEE399 pKa = 4.15RR400 pKa = 11.84PIPATITYY408 pKa = 8.34HH409 pKa = 5.89KK410 pKa = 9.69KK411 pKa = 9.51GVVMMTSPYY420 pKa = 9.61FLCAMVRR427 pKa = 11.84LLYY430 pKa = 10.08VLKK433 pKa = 10.74SKK435 pKa = 10.49FVVPTGKK442 pKa = 9.7YY443 pKa = 8.95HH444 pKa = 7.2QIFHH448 pKa = 6.94MDD450 pKa = 3.56PEE452 pKa = 4.24RR453 pKa = 11.84LEE455 pKa = 3.78VSNFFKK461 pKa = 10.9EE462 pKa = 3.6IDD464 pKa = 3.48FSKK467 pKa = 10.51FDD469 pKa = 3.37KK470 pKa = 11.04SQGRR474 pKa = 11.84LHH476 pKa = 7.1HH477 pKa = 7.07DD478 pKa = 3.4VQFKK482 pKa = 10.59LFLMLGIPEE491 pKa = 4.6HH492 pKa = 6.74FVTSWFNAHH501 pKa = 6.13EE502 pKa = 4.31LSHH505 pKa = 8.05IRR507 pKa = 11.84DD508 pKa = 3.65RR509 pKa = 11.84DD510 pKa = 3.8CGVGFSVDD518 pKa = 3.47YY519 pKa = 10.5QRR521 pKa = 11.84RR522 pKa = 11.84TGDD525 pKa = 2.74ACTYY529 pKa = 10.46LGNTLVTLSVLSYY542 pKa = 11.16VYY544 pKa = 10.73DD545 pKa = 4.08LSNPNISFVAASGDD559 pKa = 3.52DD560 pKa = 3.7SLIGSLQPLPRR571 pKa = 11.84DD572 pKa = 3.73KK573 pKa = 11.36EE574 pKa = 4.23DD575 pKa = 3.51LCVSLFNFEE584 pKa = 4.08TKK586 pKa = 10.2FPHH589 pKa = 5.6NQPFICSKK597 pKa = 9.9FLLVVEE603 pKa = 5.11CDD605 pKa = 3.75DD606 pKa = 3.73GTKK609 pKa = 10.35EE610 pKa = 3.99VLAVPNPLKK619 pKa = 10.61LLQKK623 pKa = 10.43LGPKK627 pKa = 8.78TMQVTMIDD635 pKa = 3.82DD636 pKa = 4.9YY637 pKa = 11.5YY638 pKa = 11.61QSLCDD643 pKa = 3.68ILWVFEE649 pKa = 4.4DD650 pKa = 3.94ADD652 pKa = 3.61VCRR655 pKa = 11.84RR656 pKa = 11.84VAEE659 pKa = 4.06LAEE662 pKa = 3.93FRR664 pKa = 11.84AFKK667 pKa = 10.53GRR669 pKa = 11.84KK670 pKa = 8.3KK671 pKa = 10.84CLFLEE676 pKa = 4.48SALLSLPSLVANRR689 pKa = 11.84LKK691 pKa = 10.7FLRR694 pKa = 11.84RR695 pKa = 11.84TINLEE700 pKa = 3.69SSKK703 pKa = 11.33ACIRR707 pKa = 11.84NDD709 pKa = 3.17VYY711 pKa = 11.84SNLVDD716 pKa = 3.98ALEE719 pKa = 4.28PMCARR724 pKa = 11.84RR725 pKa = 11.84LDD727 pKa = 3.71ASPRR731 pKa = 11.84RR732 pKa = 11.84SEE734 pKa = 3.68ICSGNEE740 pKa = 3.34KK741 pKa = 10.16VRR743 pKa = 11.84IGSRR747 pKa = 11.84NSRR750 pKa = 11.84CGSWRR755 pKa = 11.84KK756 pKa = 8.97EE757 pKa = 3.41YY758 pKa = 10.51RR759 pKa = 11.84AGDD762 pKa = 3.75KK763 pKa = 10.69IEE765 pKa = 4.01TPRR768 pKa = 11.84ICEE771 pKa = 4.09GVHH774 pKa = 5.42GRR776 pKa = 11.84GKK778 pKa = 10.33RR779 pKa = 11.84EE780 pKa = 3.66PSANPQRR787 pKa = 11.84ISFRR791 pKa = 11.84GKK793 pKa = 8.82EE794 pKa = 4.02LTWKK798 pKa = 10.37GGNKK802 pKa = 9.85LPP804 pKa = 4.75

Molecular weight: 92.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2QF33|Q2QF33_9BROM Movement protein OS=Blackberry chlorotic ringspot virus OX=339420 PE=2 SV=1
MM1 pKa = 7.13STLTKK6 pKa = 11.03NPDD9 pKa = 3.37QPSNAMSANRR19 pKa = 11.84GRR21 pKa = 11.84NGISNSNRR29 pKa = 11.84GCPNCFDD36 pKa = 4.56NLDD39 pKa = 3.87AAASNCTRR47 pKa = 11.84CNPAVSNRR55 pKa = 11.84QRR57 pKa = 11.84RR58 pKa = 11.84NARR61 pKa = 11.84RR62 pKa = 11.84AANFRR67 pKa = 11.84NQRR70 pKa = 11.84KK71 pKa = 9.03FDD73 pKa = 3.79GLRR76 pKa = 11.84ALQAPVPLPVVPVPQPATQRR96 pKa = 11.84NLRR99 pKa = 11.84LPNGQVWVTRR109 pKa = 11.84KK110 pKa = 7.97PTDD113 pKa = 2.9WAAKK117 pKa = 10.44VNDD120 pKa = 4.59ANDD123 pKa = 3.2AMLLKK128 pKa = 10.04TIFDD132 pKa = 5.55GITEE136 pKa = 4.7IKK138 pKa = 10.15PDD140 pKa = 3.43TKK142 pKa = 10.72VFRR145 pKa = 11.84VLIGFVAMSDD155 pKa = 3.62GTFGLVDD162 pKa = 4.29GVTGDD167 pKa = 4.01TVPEE171 pKa = 3.91LPIIGRR177 pKa = 11.84WSFQRR182 pKa = 11.84DD183 pKa = 3.66VYY185 pKa = 10.58RR186 pKa = 11.84SRR188 pKa = 11.84DD189 pKa = 3.31IGLDD193 pKa = 3.54GQPADD198 pKa = 3.75QLSEE202 pKa = 4.19KK203 pKa = 10.67AVVWCLNTNKK213 pKa = 10.09RR214 pKa = 11.84AEE216 pKa = 4.1KK217 pKa = 10.01RR218 pKa = 11.84VRR220 pKa = 11.84LADD223 pKa = 3.92FWVAIAKK230 pKa = 7.5PKK232 pKa = 10.38PLMPPPDD239 pKa = 4.16FLVEE243 pKa = 4.42DD244 pKa = 3.88NN245 pKa = 4.33

Molecular weight: 27.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2652

211

1093

530.4

59.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.41 ± 1.073

2.262 ± 0.182

7.315 ± 0.611

5.882 ± 0.664

4.299 ± 0.409

4.299 ± 0.258

2.376 ± 0.41

5.204 ± 0.343

6.184 ± 0.331

8.71 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.187

4.751 ± 0.579

5.166 ± 0.988

2.376 ± 0.3

6.109 ± 0.471

7.541 ± 0.439

5.807 ± 0.559

8.71 ± 0.174

1.131 ± 0.153

2.828 ± 0.579
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski