bacterium 1xD8-6

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4329 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A8YAH8|A0A3A8YAH8_9BACT Uncharacterized protein OS=bacterium 1xD8-6 OX=2320116 GN=D7V82_22875 PE=4 SV=1
MM1 pKa = 7.32LVVSKK6 pKa = 11.05DD7 pKa = 3.58SDD9 pKa = 3.63WEE11 pKa = 4.03KK12 pKa = 11.0SFEE15 pKa = 4.0NEE17 pKa = 3.88RR18 pKa = 11.84NVVICDD24 pKa = 4.09SISSANNMLNSIDD37 pKa = 5.09CILDD41 pKa = 3.28NAIVEE46 pKa = 4.28KK47 pKa = 10.82LNTKK51 pKa = 9.33MYY53 pKa = 10.92QEE55 pKa = 4.72MEE57 pKa = 4.09NSIHH61 pKa = 6.61SLVEE65 pKa = 4.1SEE67 pKa = 5.05SYY69 pKa = 10.46TIGDD73 pKa = 3.8YY74 pKa = 11.02EE75 pKa = 4.19ILDD78 pKa = 3.95DD79 pKa = 4.16VEE81 pKa = 4.59IDD83 pKa = 2.99IVEE86 pKa = 3.69ISEE89 pKa = 4.01IYY91 pKa = 10.67NYY93 pKa = 10.02IPLKK97 pKa = 9.04ITHH100 pKa = 6.47SSILMKK106 pKa = 9.97VTVSLSVDD114 pKa = 3.02GSGIILNEE122 pKa = 4.5DD123 pKa = 2.72NSYY126 pKa = 10.75WDD128 pKa = 4.94DD129 pKa = 3.56EE130 pKa = 4.82DD131 pKa = 4.4GVYY134 pKa = 10.59LFKK137 pKa = 11.11SFEE140 pKa = 4.11NLVFTNGLAEE150 pKa = 4.31IDD152 pKa = 4.19CEE154 pKa = 4.57VLLTYY159 pKa = 10.98DD160 pKa = 4.2FDD162 pKa = 4.6NPLEE166 pKa = 4.29TVQLEE171 pKa = 4.14DD172 pKa = 3.25VTLNNSTFPAA182 pKa = 4.22

Molecular weight: 20.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A8ZC73|A0A3A8ZC73_9BACT Serine protease OS=bacterium 1xD8-6 OX=2320116 GN=D7V82_02105 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.6KK9 pKa = 7.44RR10 pKa = 11.84HH11 pKa = 5.68RR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.09GFRR19 pKa = 11.84KK20 pKa = 9.88RR21 pKa = 11.84MHH23 pKa = 6.07TANGRR28 pKa = 11.84KK29 pKa = 8.95ILANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.27GRR39 pKa = 11.84HH40 pKa = 5.48KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4329

0

4329

1321254

20

3056

305.2

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.13 ± 0.039

1.533 ± 0.016

5.631 ± 0.034

7.741 ± 0.045

4.2 ± 0.031

6.855 ± 0.037

1.712 ± 0.016

7.384 ± 0.037

7.353 ± 0.041

8.808 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.047 ± 0.02

4.559 ± 0.027

2.994 ± 0.02

3.414 ± 0.023

4.66 ± 0.03

6.097 ± 0.03

4.972 ± 0.031

6.59 ± 0.031

0.934 ± 0.013

4.386 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski