Thamnocephalis sphaerospora

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Zoopagomycotina; Zoopagomycetes; Zoopagales; Sigmoideomycetaceae; Thamnocephalis

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6769 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P9XRN4|A0A4P9XRN4_9FUNG Thiolase N-terminal domain-containing protein OS=Thamnocephalis sphaerospora OX=78915 GN=THASP1DRAFT_29466 PE=3 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.31RR4 pKa = 11.84ATSCSAPSEE13 pKa = 4.32TMVPDD18 pKa = 3.34IDD20 pKa = 3.99YY21 pKa = 10.54AIPDD25 pKa = 3.41SSDD28 pKa = 2.68NRR30 pKa = 11.84RR31 pKa = 11.84SSGAYY36 pKa = 8.83VAGSGYY42 pKa = 10.33QYY44 pKa = 11.21SNHH47 pKa = 5.49STNNDD52 pKa = 3.07ADD54 pKa = 4.11GSDD57 pKa = 3.75SQGSDD62 pKa = 2.66NN63 pKa = 4.74

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P9XN83|A0A4P9XN83_9FUNG Uncharacterized protein OS=Thamnocephalis sphaerospora OX=78915 GN=THASP1DRAFT_24461 PE=4 SV=1
MM1 pKa = 7.74FLRR4 pKa = 11.84TLLARR9 pKa = 11.84ATVAAPLLHH18 pKa = 6.65RR19 pKa = 11.84AARR22 pKa = 11.84PAAITAAPMTTKK34 pKa = 10.11FRR36 pKa = 11.84WPLQGFARR44 pKa = 11.84PQPLALAGSQPAGVAGFATSTTVQSQFAVNRR75 pKa = 11.84KK76 pKa = 8.99RR77 pKa = 11.84RR78 pKa = 11.84TARR81 pKa = 11.84LKK83 pKa = 10.92AKK85 pKa = 10.48LKK87 pKa = 10.57AKK89 pKa = 9.3QKK91 pKa = 10.24RR92 pKa = 11.84LRR94 pKa = 11.84RR95 pKa = 11.84DD96 pKa = 3.06KK97 pKa = 11.47TNGPWKK103 pKa = 10.59

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6769

0

6769

2686534

50

4653

396.9

43.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.539 ± 0.037

1.527 ± 0.012

5.734 ± 0.022

5.74 ± 0.033

3.322 ± 0.019

6.363 ± 0.026

2.681 ± 0.015

4.263 ± 0.022

3.763 ± 0.026

9.436 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.012

3.171 ± 0.018

5.408 ± 0.038

4.167 ± 0.022

7.135 ± 0.028

7.409 ± 0.034

5.972 ± 0.02

6.78 ± 0.025

1.33 ± 0.012

2.787 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski