Apis mellifera associated microvirus 57

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTM2|A0A3S8UTM2_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 57 OX=2494788 PE=4 SV=1
MM1 pKa = 7.53LMKK4 pKa = 10.47KK5 pKa = 9.92IFILVDD11 pKa = 3.78RR12 pKa = 11.84VANQLSDD19 pKa = 3.16VCMFEE24 pKa = 4.07NDD26 pKa = 3.29KK27 pKa = 10.97QALRR31 pKa = 11.84TFRR34 pKa = 11.84AIYY37 pKa = 10.48DD38 pKa = 3.63KK39 pKa = 11.73AHH41 pKa = 5.9VDD43 pKa = 3.25KK44 pKa = 11.6DD45 pKa = 3.5NGRR48 pKa = 11.84LSLMRR53 pKa = 11.84DD54 pKa = 3.15CDD56 pKa = 3.65MYY58 pKa = 11.25QYY60 pKa = 11.15DD61 pKa = 4.31YY62 pKa = 9.67EE63 pKa = 4.54TGVKK67 pKa = 9.92RR68 pKa = 11.84IVPEE72 pKa = 3.95YY73 pKa = 10.75QLMVADD79 pKa = 4.29KK80 pKa = 10.66DD81 pKa = 3.98YY82 pKa = 11.48GVKK85 pKa = 10.47DD86 pKa = 3.54EE87 pKa = 4.83NEE89 pKa = 3.95NNEE92 pKa = 3.97

Molecular weight: 10.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U5L7|A0A3Q8U5L7_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 57 OX=2494788 PE=4 SV=1
MM1 pKa = 7.39YY2 pKa = 10.04IITHH6 pKa = 6.81IGDD9 pKa = 3.45KK10 pKa = 9.59MNKK13 pKa = 9.15RR14 pKa = 11.84KK15 pKa = 9.72ARR17 pKa = 11.84KK18 pKa = 9.03LKK20 pKa = 9.67AGYY23 pKa = 8.81FRR25 pKa = 11.84KK26 pKa = 9.61HH27 pKa = 5.68ANKK30 pKa = 9.7TNIVNTGVITKK41 pKa = 10.34RR42 pKa = 11.84GGFRR46 pKa = 11.84FF47 pKa = 3.69

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

407

47

194

101.8

11.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.143 ± 1.851

0.491 ± 0.435

7.125 ± 1.652

6.634 ± 1.631

3.686 ± 0.816

4.914 ± 1.171

1.474 ± 0.743

6.388 ± 1.019

11.548 ± 1.383

7.125 ± 0.978

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.44 ± 0.889

7.617 ± 0.299

3.194 ± 1.033

4.423 ± 1.236

4.177 ± 1.684

3.931 ± 1.332

7.371 ± 1.752

6.143 ± 1.258

0.0 ± 0.0

4.177 ± 0.75
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski