Streptococcus satellite phage Javan229

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHV6|A0A4D5ZHV6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan229 OX=2558582 GN=JavanS229_0012 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.13IKK4 pKa = 10.65LFNRR8 pKa = 11.84EE9 pKa = 4.09QIKK12 pKa = 10.66SKK14 pKa = 10.37YY15 pKa = 10.33DD16 pKa = 3.38EE17 pKa = 4.23VLSITKK23 pKa = 10.49LEE25 pKa = 4.26TNEE28 pKa = 3.81EE29 pKa = 4.04LEE31 pKa = 4.0NRR33 pKa = 11.84VNSFIADD40 pKa = 3.59KK41 pKa = 10.88KK42 pKa = 11.04VIDD45 pKa = 4.05IKK47 pKa = 10.89YY48 pKa = 9.89QEE50 pKa = 4.25ATYY53 pKa = 8.55GTYY56 pKa = 11.01EE57 pKa = 4.23EE58 pKa = 4.13MDD60 pKa = 3.7AYY62 pKa = 10.24LTIIVMYY69 pKa = 10.5EE70 pKa = 3.76EE71 pKa = 4.42VTNMTNTNKK80 pKa = 8.0TTAPMTLAEE89 pKa = 4.13LKK91 pKa = 9.89EE92 pKa = 4.39WVEE95 pKa = 4.43EE96 pKa = 4.17TCEE99 pKa = 5.14LSTLLQTKK107 pKa = 9.55DD108 pKa = 3.44YY109 pKa = 10.95KK110 pKa = 11.14SYY112 pKa = 10.96LPEE115 pKa = 4.1EE116 pKa = 4.17KK117 pKa = 10.12QEE119 pKa = 4.0EE120 pKa = 4.4FEE122 pKa = 5.4AIAFSVFNCLEE133 pKa = 4.21SFSMMLEE140 pKa = 4.12DD141 pKa = 6.33DD142 pKa = 4.05EE143 pKa = 6.14LHH145 pKa = 5.83YY146 pKa = 10.52QPKK149 pKa = 9.85PIEE152 pKa = 4.32EE153 pKa = 4.24EE154 pKa = 3.95SDD156 pKa = 3.39LDD158 pKa = 3.84NQADD162 pKa = 3.7EE163 pKa = 4.09MAQYY167 pKa = 10.39HH168 pKa = 5.82EE169 pKa = 4.49LMKK172 pKa = 10.62EE173 pKa = 4.06VEE175 pKa = 4.63DD176 pKa = 3.69SDD178 pKa = 4.32KK179 pKa = 10.63EE180 pKa = 4.17ARR182 pKa = 11.84EE183 pKa = 3.6IAYY186 pKa = 10.13DD187 pKa = 3.85DD188 pKa = 4.75TIHH191 pKa = 7.14EE192 pKa = 4.79YY193 pKa = 9.73IDD195 pKa = 3.8KK196 pKa = 9.55RR197 pKa = 11.84TEE199 pKa = 3.86QLKK202 pKa = 9.45EE203 pKa = 3.72QADD206 pKa = 3.87VEE208 pKa = 4.34EE209 pKa = 4.86LVNKK213 pKa = 7.87VARR216 pKa = 11.84YY217 pKa = 6.97EE218 pKa = 4.12AEE220 pKa = 4.46LIDD223 pKa = 3.8YY224 pKa = 10.69AEE226 pKa = 4.77RR227 pKa = 11.84LLSDD231 pKa = 3.74EE232 pKa = 4.79PLNADD237 pKa = 3.49SEE239 pKa = 4.84TAIGTLDD246 pKa = 3.63MLDD249 pKa = 4.84DD250 pKa = 3.92EE251 pKa = 6.32AIDD254 pKa = 3.89LFKK257 pKa = 11.28SVDD260 pKa = 3.22VDD262 pKa = 3.55NEE264 pKa = 4.36YY265 pKa = 11.0QGLEE269 pKa = 4.14YY270 pKa = 11.23YY271 pKa = 8.19NTSLNKK277 pKa = 9.95EE278 pKa = 4.21DD279 pKa = 3.57

Molecular weight: 32.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZM38|A0A4D5ZM38_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan229 OX=2558582 GN=JavanS229_0014 PE=3 SV=1
MM1 pKa = 7.51NIKK4 pKa = 10.04EE5 pKa = 4.29VIKK8 pKa = 10.83KK9 pKa = 10.43DD10 pKa = 3.21GTKK13 pKa = 9.85VYY15 pKa = 10.42RR16 pKa = 11.84SNVYY20 pKa = 10.53LGVDD24 pKa = 3.99SITGKK29 pKa = 10.34KK30 pKa = 10.21ARR32 pKa = 11.84TSVTARR38 pKa = 11.84TKK40 pKa = 10.93KK41 pKa = 9.82EE42 pKa = 3.91LKK44 pKa = 10.19QKK46 pKa = 10.6AKK48 pKa = 10.17QAITAFINNGYY59 pKa = 5.21TTKK62 pKa = 10.34TKK64 pKa = 10.73TNIRR68 pKa = 11.84TYY70 pKa = 11.15KK71 pKa = 9.7EE72 pKa = 3.7LAYY75 pKa = 10.49LWWDD79 pKa = 3.34SCKK82 pKa = 9.88NTVKK86 pKa = 10.52INTRR90 pKa = 11.84KK91 pKa = 9.89AVEE94 pKa = 3.95GQIRR98 pKa = 11.84VHH100 pKa = 6.67LLPAFGDD107 pKa = 3.81YY108 pKa = 10.62KK109 pKa = 10.88LEE111 pKa = 4.17KK112 pKa = 9.34LTTPIIQQQVNKK124 pKa = 9.07WASDD128 pKa = 3.61YY129 pKa = 11.78NKK131 pKa = 10.42GKK133 pKa = 10.2KK134 pKa = 9.95GSYY137 pKa = 8.57KK138 pKa = 10.37HH139 pKa = 6.14YY140 pKa = 10.4NQLHH144 pKa = 6.17ALNKK148 pKa = 10.23RR149 pKa = 11.84ILQYY153 pKa = 11.28AVIMQLIPFNPARR166 pKa = 11.84EE167 pKa = 4.22VIVPRR172 pKa = 11.84KK173 pKa = 9.66KK174 pKa = 9.0QTEE177 pKa = 3.96EE178 pKa = 3.85VKK180 pKa = 10.59IKK182 pKa = 10.63FLDD185 pKa = 3.7KK186 pKa = 11.18QEE188 pKa = 4.57LKK190 pKa = 10.68QFYY193 pKa = 10.2DD194 pKa = 3.74YY195 pKa = 11.63LDD197 pKa = 3.84TLDD200 pKa = 3.24KK201 pKa = 10.91SKK203 pKa = 11.21YY204 pKa = 8.38YY205 pKa = 11.15NLFDD209 pKa = 3.55VVLYY213 pKa = 8.25KK214 pKa = 10.5TLLATGCRR222 pKa = 11.84IGEE225 pKa = 4.15VLALEE230 pKa = 4.42WSDD233 pKa = 3.44IDD235 pKa = 5.15LDD237 pKa = 3.77NGYY240 pKa = 10.33ISINKK245 pKa = 7.82TLNRR249 pKa = 11.84DD250 pKa = 3.77DD251 pKa = 4.94EE252 pKa = 5.09VNCPKK257 pKa = 10.71SKK259 pKa = 10.67AGYY262 pKa = 9.69RR263 pKa = 11.84DD264 pKa = 3.13ISIDD268 pKa = 3.12KK269 pKa = 9.51ATILMLKK276 pKa = 9.61HH277 pKa = 5.3YY278 pKa = 10.2KK279 pKa = 9.78NRR281 pKa = 11.84QTIQAWQHH289 pKa = 5.19KK290 pKa = 8.15RR291 pKa = 11.84TEE293 pKa = 4.35TVVFSTFVNKK303 pKa = 10.04YY304 pKa = 9.36ASQPALRR311 pKa = 11.84RR312 pKa = 11.84RR313 pKa = 11.84LEE315 pKa = 3.89RR316 pKa = 11.84HH317 pKa = 5.84LKK319 pKa = 8.68NAGVSYY325 pKa = 11.11VSFHH329 pKa = 6.23GLRR332 pKa = 11.84HH333 pKa = 4.44THH335 pKa = 5.07ATIMLNAGIQPKK347 pKa = 10.11DD348 pKa = 3.26LQYY351 pKa = 11.59RR352 pKa = 11.84LGHH355 pKa = 6.14SDD357 pKa = 3.42ISMTLNTYY365 pKa = 8.7VHH367 pKa = 6.14ATKK370 pKa = 10.58EE371 pKa = 4.12GAKK374 pKa = 10.15NSASFFEE381 pKa = 4.46SAINSLGG388 pKa = 3.34

Molecular weight: 44.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2237

48

542

159.8

18.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.811 ± 0.345

0.581 ± 0.101

6.08 ± 0.599

8.494 ± 1.458

4.157 ± 0.509

4.068 ± 0.613

1.565 ± 0.313

7.734 ± 0.472

9.566 ± 0.618

10.326 ± 0.68

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.387

5.856 ± 0.31

3.174 ± 0.599

4.426 ± 0.272

4.247 ± 0.369

4.336 ± 0.353

5.99 ± 0.528

4.783 ± 0.423

0.894 ± 0.141

5.633 ± 0.572
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski