Candidatus Brocadiaceae bacterium S225

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Candidatus Brocadiae; Candidatus Brocadiales; Candidatus Brocadiaceae; unclassified Candidatus Brocadiaceae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2768 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6D5K1|A0A5C6D5K1_9BACT Cobalt-precorrin-2 C(20)-methyltransferase OS=Candidatus Brocadiaceae bacterium S225 OX=2528033 GN=cbiL PE=3 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84IKK4 pKa = 10.96NFIIAVLVAVVVGVSGGLATAQITNFSTDD33 pKa = 2.01VDD35 pKa = 3.93TAINRR40 pKa = 11.84GLTFQDD46 pKa = 2.97SSGSYY51 pKa = 9.06TGSAGDD57 pKa = 3.67ATGLALLSLLEE68 pKa = 4.27KK69 pKa = 10.4RR70 pKa = 11.84VSADD74 pKa = 3.13QNAAPQGYY82 pKa = 9.7VNANAADD89 pKa = 3.84QNRR92 pKa = 11.84MNSAVDD98 pKa = 4.6FIIDD102 pKa = 4.05EE103 pKa = 4.06NRR105 pKa = 11.84EE106 pKa = 3.99DD107 pKa = 3.87NGCGGEE113 pKa = 4.71DD114 pKa = 4.19EE115 pKa = 5.31NCAPCNTEE123 pKa = 3.65WADD126 pKa = 3.2GDD128 pKa = 3.92AADD131 pKa = 3.83WQNLSFEE138 pKa = 4.68PFRR141 pKa = 11.84DD142 pKa = 3.18WSGRR146 pKa = 11.84GQGPNMLGRR155 pKa = 11.84PAVLHH160 pKa = 6.58LIEE163 pKa = 4.51EE164 pKa = 4.91DD165 pKa = 3.12IYY167 pKa = 11.5LDD169 pKa = 4.45VEE171 pKa = 4.18FTLWDD176 pKa = 3.37TCGFGSEE183 pKa = 4.77GEE185 pKa = 4.4GEE187 pKa = 4.43GEE189 pKa = 4.35GEE191 pKa = 4.4GEE193 pKa = 4.39GEE195 pKa = 4.47GEE197 pKa = 4.37GGSGSAVQVGGCSPFPGFQYY217 pKa = 10.67LRR219 pKa = 11.84STEE222 pKa = 4.15GVPGEE227 pKa = 4.28KK228 pKa = 9.92VWTGGQITFTKK239 pKa = 10.19PCGADD244 pKa = 3.0PDD246 pKa = 4.27VPANGDD252 pKa = 3.61GITAFTRR259 pKa = 11.84LTRR262 pKa = 11.84GDD264 pKa = 3.75CGSIYY269 pKa = 10.78NSVCEE274 pKa = 3.86EE275 pKa = 4.36DD276 pKa = 4.5FFNSAPCPGGPGGLPFYY293 pKa = 10.5AYY295 pKa = 10.3RR296 pKa = 11.84DD297 pKa = 4.17GQDD300 pKa = 2.88MMALSVYY307 pKa = 9.87RR308 pKa = 11.84RR309 pKa = 11.84TGGPIDD315 pKa = 3.95VLPALLAIFDD325 pKa = 3.99RR326 pKa = 11.84TIAAQAPDD334 pKa = 5.44GYY336 pKa = 10.1WCYY339 pKa = 11.05CDD341 pKa = 5.25GGCSDD346 pKa = 3.96SSTTQFVIAGLAAIRR361 pKa = 11.84AVLIDD366 pKa = 3.91EE367 pKa = 4.62NDD369 pKa = 3.59FARR372 pKa = 11.84IAQLDD377 pKa = 3.86AATALCRR384 pKa = 11.84AAYY387 pKa = 10.13ASIPAQNPVLTPGEE401 pKa = 4.28LGHH404 pKa = 6.76GYY406 pKa = 10.14NRR408 pKa = 11.84GNQNSLQQTGSGTWIQLAGGADD430 pKa = 3.75LNDD433 pKa = 4.35AGVQAYY439 pKa = 9.19LRR441 pKa = 11.84WLYY444 pKa = 11.04NRR446 pKa = 11.84YY447 pKa = 9.21NDD449 pKa = 4.72RR450 pKa = 11.84SILGAGGGWSGSYY463 pKa = 10.24FYY465 pKa = 10.51YY466 pKa = 10.4LWSFAKK472 pKa = 10.62ALTFLEE478 pKa = 4.75DD479 pKa = 3.62SGITANAGNLAVADD493 pKa = 4.97LGTLDD498 pKa = 4.19PAAVPAFGGRR508 pKa = 11.84VLHH511 pKa = 7.06RR512 pKa = 11.84DD513 pKa = 3.3PTTVPRR519 pKa = 11.84PAVFGPDD526 pKa = 2.96PGAYY530 pKa = 9.8YY531 pKa = 10.31DD532 pKa = 5.48DD533 pKa = 5.1PNEE536 pKa = 3.96PPRR539 pKa = 11.84WYY541 pKa = 10.66FDD543 pKa = 3.31FAYY546 pKa = 10.7SLLLLQDD553 pKa = 3.73ASGQFLNPAGHH564 pKa = 6.5GNWDD568 pKa = 3.21NAARR572 pKa = 11.84QAYY575 pKa = 9.39SLLVLVRR582 pKa = 11.84SVGGGCLDD590 pKa = 3.38TDD592 pKa = 4.13GDD594 pKa = 4.29QVCDD598 pKa = 4.72SEE600 pKa = 5.31DD601 pKa = 3.53NCPADD606 pKa = 4.08ANPGQEE612 pKa = 5.42DD613 pKa = 4.12GDD615 pKa = 4.13SDD617 pKa = 5.05DD618 pKa = 5.44VGDD621 pKa = 4.6VCDD624 pKa = 3.95NCINTPNPGQEE635 pKa = 4.95DD636 pKa = 4.1GDD638 pKa = 4.05SDD640 pKa = 4.63GVGDD644 pKa = 5.06ACDD647 pKa = 3.87NCPDD651 pKa = 4.63DD652 pKa = 5.86ANPNQDD658 pKa = 4.8DD659 pKa = 4.07GDD661 pKa = 4.3GDD663 pKa = 4.41GVGDD667 pKa = 3.98VCDD670 pKa = 3.92NCLDD674 pKa = 4.07APNPNQADD682 pKa = 3.62GDD684 pKa = 4.04NDD686 pKa = 4.19GLGDD690 pKa = 4.29ACDD693 pKa = 3.98NCPDD697 pKa = 4.81DD698 pKa = 5.76ANPNQGDD705 pKa = 3.43VDD707 pKa = 3.99GDD709 pKa = 4.29GVGDD713 pKa = 3.75VCDD716 pKa = 3.89NCIDD720 pKa = 3.93TPNPGQEE727 pKa = 4.57DD728 pKa = 3.71VDD730 pKa = 4.05GDD732 pKa = 4.52GVGDD736 pKa = 4.07ACDD739 pKa = 3.76TPQIPCVEE747 pKa = 4.54DD748 pKa = 3.0LTARR752 pKa = 11.84AKK754 pKa = 9.55TGQVTLVWTHH764 pKa = 5.94VGADD768 pKa = 3.42SYY770 pKa = 11.86NVYY773 pKa = 10.72SGLNPGGPYY782 pKa = 10.76GLIGNTTSTYY792 pKa = 9.45STYY795 pKa = 10.63TDD797 pKa = 2.97SGLTNGITVYY807 pKa = 10.79YY808 pKa = 9.74IVRR811 pKa = 11.84PVLGGQEE818 pKa = 4.15LCPVTGNEE826 pKa = 4.24ASEE829 pKa = 4.57TPQEE833 pKa = 3.73RR834 pKa = 11.84RR835 pKa = 11.84RR836 pKa = 11.84RR837 pKa = 11.84EE838 pKa = 3.62RR839 pKa = 3.48

Molecular weight: 88.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6DFI0|A0A5C6DFI0_9BACT Hydroxylamine reductase OS=Candidatus Brocadiaceae bacterium S225 OX=2528033 GN=hcp PE=4 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.57LKK5 pKa = 8.67THH7 pKa = 6.6KK8 pKa = 10.54GLTKK12 pKa = 10.15RR13 pKa = 11.84VRR15 pKa = 11.84VSSNGKK21 pKa = 9.2IKK23 pKa = 10.41RR24 pKa = 11.84KK25 pKa = 9.42KK26 pKa = 10.17AGAGHH31 pKa = 7.33LMSGKK36 pKa = 8.08TGSRR40 pKa = 11.84KK41 pKa = 8.51RR42 pKa = 11.84TLKK45 pKa = 9.78RR46 pKa = 11.84TSLVSKK52 pKa = 10.42GFTRR56 pKa = 11.84SVLRR60 pKa = 11.84ALGKK64 pKa = 10.33AA65 pKa = 3.52

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2768

0

2768

835022

29

2953

301.7

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.381 ± 0.04

1.296 ± 0.023

5.639 ± 0.037

6.952 ± 0.045

4.509 ± 0.037

6.79 ± 0.044

1.982 ± 0.022

8.416 ± 0.049

7.591 ± 0.054

9.351 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.024

4.79 ± 0.035

3.584 ± 0.025

2.851 ± 0.023

4.637 ± 0.037

6.463 ± 0.035

5.288 ± 0.037

6.612 ± 0.039

1.002 ± 0.016

3.404 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski