Bat paramyxovirus
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346NTM2|A0A346NTM2_9MONO Matrix protein OS=Bat paramyxovirus OX=1300978 GN=M PE=4 SV=1
MM1 pKa = 7.34 SRR3 pKa = 11.84 VDD5 pKa = 3.85 KK6 pKa = 11.23 LSASVDD12 pKa = 3.19 NGLAIAEE19 pKa = 4.73 FIQEE23 pKa = 4.14 NRR25 pKa = 11.84 SSIQSTYY32 pKa = 9.5 GRR34 pKa = 11.84 SSIGEE39 pKa = 3.66 PTIANRR45 pKa = 11.84 AKK47 pKa = 10.42 AWEE50 pKa = 4.3 LYY52 pKa = 9.96 IDD54 pKa = 3.71 RR55 pKa = 11.84 TNSRR59 pKa = 11.84 NAGHH63 pKa = 6.45 EE64 pKa = 4.39 GKK66 pKa = 10.48 LKK68 pKa = 10.69 SINEE72 pKa = 3.98 LPEE75 pKa = 4.44 GKK77 pKa = 10.33 GSEE80 pKa = 4.22 TTRR83 pKa = 11.84 VAEE86 pKa = 4.04 TSEE89 pKa = 4.4 IEE91 pKa = 4.63 QSPKK95 pKa = 10.12 NQNSSSGDD103 pKa = 3.23 GSGDD107 pKa = 3.44 FNFRR111 pKa = 11.84 FPGDD115 pKa = 3.5 SSEE118 pKa = 4.31 SDD120 pKa = 2.72 IGVWSKK126 pKa = 11.41 NSSDD130 pKa = 3.58 LSDD133 pKa = 3.47 VEE135 pKa = 4.4 GRR137 pKa = 11.84 SISISEE143 pKa = 4.32 GEE145 pKa = 4.42 AEE147 pKa = 4.54 PEE149 pKa = 4.09 TSNDD153 pKa = 2.77 VPSQVNEE160 pKa = 3.89 SSRR163 pKa = 11.84 DD164 pKa = 3.58 HH165 pKa = 6.59 ARR167 pKa = 11.84 EE168 pKa = 3.91 GADD171 pKa = 3.26 QADD174 pKa = 4.08 EE175 pKa = 4.2 QVVSSNDD182 pKa = 3.25 QEE184 pKa = 4.32 TTTIIEE190 pKa = 4.34 NPSKK194 pKa = 10.83 EE195 pKa = 4.34 SIASIMEE202 pKa = 3.93 EE203 pKa = 4.09 SGPMPKK209 pKa = 9.51 RR210 pKa = 11.84 RR211 pKa = 11.84 LKK213 pKa = 9.35 NASRR217 pKa = 11.84 IKK219 pKa = 9.49 EE220 pKa = 4.32 TVDD223 pKa = 4.76 AIPADD228 pKa = 3.77 TPIVKK233 pKa = 8.68 KK234 pKa = 7.56 TTGEE238 pKa = 3.99 NIQLQKK244 pKa = 10.3 KK245 pKa = 6.43 TDD247 pKa = 3.67 KK248 pKa = 11.42 LEE250 pKa = 4.31 LSAGATQSAHH260 pKa = 7.28 PSPQIQSSTNADD272 pKa = 2.59 VDD274 pKa = 3.9 TAQDD278 pKa = 3.38 HH279 pKa = 4.18 VHH281 pKa = 5.35 YY282 pKa = 10.88 AKK284 pKa = 8.46 MTEE287 pKa = 4.12 EE288 pKa = 4.04 SHH290 pKa = 6.87 PLSKK294 pKa = 10.01 TDD296 pKa = 3.38 TNQIALEE303 pKa = 4.17 KK304 pKa = 10.48 KK305 pKa = 9.88 LDD307 pKa = 4.31 KK308 pKa = 10.8 ILEE311 pKa = 4.22 NQEE314 pKa = 4.45 KK315 pKa = 9.91 ILKK318 pKa = 9.24 KK319 pKa = 10.72 LEE321 pKa = 3.9 VLSEE325 pKa = 4.1 VKK327 pKa = 10.84 EE328 pKa = 3.89 EE329 pKa = 3.84 VNAIKK334 pKa = 9.21 KK335 pKa = 7.37 TLSNQSLALSTIEE348 pKa = 4.15 NYY350 pKa = 9.67 IGEE353 pKa = 4.37 MMVIIPKK360 pKa = 10.31 SGVPEE365 pKa = 4.37 DD366 pKa = 4.17 EE367 pKa = 4.49 NNHH370 pKa = 6.65 KK371 pKa = 8.75 ITKK374 pKa = 10.19 NPDD377 pKa = 2.86 LRR379 pKa = 11.84 PVIGRR384 pKa = 11.84 DD385 pKa = 3.01 STRR388 pKa = 11.84 GKK390 pKa = 10.57 KK391 pKa = 9.22 EE392 pKa = 3.83 VIRR395 pKa = 11.84 PMTDD399 pKa = 2.83 RR400 pKa = 11.84 EE401 pKa = 4.21 RR402 pKa = 11.84 IDD404 pKa = 3.32 IDD406 pKa = 3.43 EE407 pKa = 4.84 DD408 pKa = 4.64 FYY410 pKa = 11.59 NIPVVDD416 pKa = 3.8 EE417 pKa = 4.69 KK418 pKa = 11.74 YY419 pKa = 10.11 FVKK422 pKa = 10.6 PINKK426 pKa = 9.14 QEE428 pKa = 3.66 NNAANFVPKK437 pKa = 9.9 EE438 pKa = 4.14 DD439 pKa = 3.51 KK440 pKa = 9.86 TSYY443 pKa = 10.54 RR444 pKa = 11.84 IIRR447 pKa = 11.84 DD448 pKa = 3.83 IIRR451 pKa = 11.84 KK452 pKa = 7.2 EE453 pKa = 4.09 VKK455 pKa = 10.39 DD456 pKa = 3.78 PEE458 pKa = 4.49 ARR460 pKa = 11.84 DD461 pKa = 3.57 AVIKK465 pKa = 10.41 LFNDD469 pKa = 4.02 SIGKK473 pKa = 9.28 VPINEE478 pKa = 4.05 IYY480 pKa = 10.67 DD481 pKa = 4.48 GIRR484 pKa = 11.84 MLVYY488 pKa = 10.47 TDD490 pKa = 4.09 ILDD493 pKa = 4.23 HH494 pKa = 6.94 LEE496 pKa = 4.0 KK497 pKa = 10.92
Molecular weight: 55.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.039
IPC2_protein 4.965
IPC_protein 4.914
Toseland 4.8
ProMoST 5.016
Dawson 4.863
Bjellqvist 5.016
Wikipedia 4.724
Rodwell 4.774
Grimsley 4.711
Solomon 4.863
Lehninger 4.825
Nozaki 4.978
DTASelect 5.118
Thurlkill 4.787
EMBOSS 4.749
Sillero 5.054
Patrickios 4.482
IPC_peptide 4.876
IPC2_peptide 5.041
IPC2.peptide.svr19 5.042
Protein with the highest isoelectric point:
>tr|A0A346NTM3|A0A346NTM3_9MONO Fusion glycoprotein F0 OS=Bat paramyxovirus OX=1300978 GN=F PE=3 SV=1
MM1 pKa = 7.41 SGKK4 pKa = 10.4 ADD6 pKa = 3.86 FSKK9 pKa = 10.79 RR10 pKa = 11.84 SWEE13 pKa = 4.59 DD14 pKa = 2.85 GGTLQAIDD22 pKa = 3.5 ATADD26 pKa = 3.3 EE27 pKa = 4.94 KK28 pKa = 11.46 GRR30 pKa = 11.84 LIPKK34 pKa = 9.41 VRR36 pKa = 11.84 VVNPGWNDD44 pKa = 3.34 RR45 pKa = 11.84 KK46 pKa = 9.51 STGYY50 pKa = 10.5 KK51 pKa = 9.41 YY52 pKa = 10.69 LIVYY56 pKa = 7.85 GIIEE60 pKa = 4.57 EE61 pKa = 4.29 KK62 pKa = 10.76 EE63 pKa = 3.76 KK64 pKa = 11.34 DD65 pKa = 3.48 HH66 pKa = 5.87 THH68 pKa = 5.77 SAGGEE73 pKa = 3.93 PTVRR77 pKa = 11.84 KK78 pKa = 10.1 SNAPRR83 pKa = 11.84 AFGALPLGVGRR94 pKa = 11.84 SHH96 pKa = 7.63 VEE98 pKa = 3.78 PEE100 pKa = 4.31 DD101 pKa = 3.57 LLEE104 pKa = 5.44 AILKK108 pKa = 10.55 LEE110 pKa = 3.69 ITVRR114 pKa = 11.84 RR115 pKa = 11.84 TAGANEE121 pKa = 4.11 KK122 pKa = 9.9 IVYY125 pKa = 9.43 GMSRR129 pKa = 11.84 IDD131 pKa = 4.61 NILKK135 pKa = 8.97 PWAPILEE142 pKa = 4.44 NGAIFPAIKK151 pKa = 9.89 VCSNIDD157 pKa = 3.72 IVPLDD162 pKa = 4.07 HH163 pKa = 7.07 PLKK166 pKa = 10.41 LRR168 pKa = 11.84 PIFLTITLLTDD179 pKa = 3.59 AGIYY183 pKa = 9.3 KK184 pKa = 9.8 VPRR187 pKa = 11.84 SILEE191 pKa = 3.66 FRR193 pKa = 11.84 QNKK196 pKa = 9.61 AISFNLLVTLQVGSDD211 pKa = 3.73 FTSSGVKK218 pKa = 10.56 GMINEE223 pKa = 3.93 NGEE226 pKa = 4.17 RR227 pKa = 11.84 VTTFMVHH234 pKa = 5.95 IGNFVRR240 pKa = 11.84 KK241 pKa = 9.36 RR242 pKa = 11.84 GKK244 pKa = 9.99 AYY246 pKa = 10.54 SIEE249 pKa = 4.12 YY250 pKa = 10.29 CKK252 pKa = 11.04 AKK254 pKa = 9.77 VDD256 pKa = 3.62 KK257 pKa = 10.41 MDD259 pKa = 3.27 MRR261 pKa = 11.84 FSLGAVGGLSFHH273 pKa = 6.22 VRR275 pKa = 11.84 IAGKK279 pKa = 9.07 MSHH282 pKa = 5.92 MLKK285 pKa = 10.42 AQLGYY290 pKa = 10.63 HH291 pKa = 5.19 RR292 pKa = 11.84 TICYY296 pKa = 10.83 SLMDD300 pKa = 4.0 TNPYY304 pKa = 9.59 LNRR307 pKa = 11.84 VMWRR311 pKa = 11.84 SEE313 pKa = 3.75 CSILKK318 pKa = 8.08 VTAVFQPSVPKK329 pKa = 10.26 EE330 pKa = 3.51 FKK332 pKa = 10.41 IYY334 pKa = 10.8 DD335 pKa = 3.81 DD336 pKa = 3.91 VLIDD340 pKa = 3.61 NTGKK344 pKa = 10.12 ILKK347 pKa = 9.31 QQ348 pKa = 3.23
Molecular weight: 38.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.927
IPC2_protein 8.858
IPC_protein 8.756
Toseland 9.604
ProMoST 9.297
Dawson 9.838
Bjellqvist 9.516
Wikipedia 9.984
Rodwell 10.233
Grimsley 9.897
Solomon 9.853
Lehninger 9.823
Nozaki 9.677
DTASelect 9.487
Thurlkill 9.677
EMBOSS 10.014
Sillero 9.765
Patrickios 5.753
IPC_peptide 9.853
IPC2_peptide 8.053
IPC2.peptide.svr19 8.014
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5477
239
2195
684.6
77.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.076 ± 0.698
1.296 ± 0.288
6.08 ± 0.235
5.77 ± 0.592
3.834 ± 0.446
5.094 ± 0.666
1.917 ± 0.354
8.527 ± 0.438
6.865 ± 0.645
9.385 ± 0.778
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.666 ± 0.333
6.463 ± 0.455
3.944 ± 0.233
3.688 ± 0.306
5.185 ± 0.321
8.234 ± 0.57
5.879 ± 0.45
5.222 ± 0.353
1.15 ± 0.121
3.725 ± 0.431
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here