Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Metallosphaera; Metallosphaera sedula

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4YG22|A4YG22_METS5 2-oxoacid oxidoreductase (ferredoxin) OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) OX=399549 GN=Msed_1213 PE=4 SV=1
MM1 pKa = 7.58SKK3 pKa = 10.02ILLILLGLVLASGAVVLGSNVNNVTSNIIHH33 pKa = 6.33QINPAVQGQQGTHH46 pKa = 6.93DD47 pKa = 4.02NSPDD51 pKa = 3.2NSTNSTNDD59 pKa = 3.17NTPDD63 pKa = 3.18NSTAIIHH70 pKa = 7.01DD71 pKa = 3.99NSPDD75 pKa = 3.18NSTNSTYY82 pKa = 11.07DD83 pKa = 3.41DD84 pKa = 3.8SHH86 pKa = 8.71HH87 pKa = 7.65DD88 pKa = 3.74DD89 pKa = 4.18STNSTYY95 pKa = 11.04SDD97 pKa = 4.54DD98 pKa = 3.75GHH100 pKa = 8.26HH101 pKa = 7.64DD102 pKa = 3.72DD103 pKa = 5.1SYY105 pKa = 12.11QNGTYY110 pKa = 10.2TNSTYY115 pKa = 11.11DD116 pKa = 4.19DD117 pKa = 3.8SHH119 pKa = 8.41HH120 pKa = 7.49DD121 pKa = 3.37NSTNSTYY128 pKa = 11.28DD129 pKa = 3.36NSPDD133 pKa = 3.2NSTNTNSTYY142 pKa = 11.11DD143 pKa = 3.48NSPDD147 pKa = 3.43QGSSGEE153 pKa = 4.11GDD155 pKa = 3.37SS156 pKa = 4.3

Molecular weight: 16.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4YIA4|A4YIA4_METS5 FeoA family protein OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) OX=399549 GN=Msed_2016 PE=3 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84LGKK5 pKa = 9.99ILGEE9 pKa = 3.81NRR11 pKa = 11.84SFRR14 pKa = 11.84FYY16 pKa = 9.67WASISLSHH24 pKa = 7.27ISGIMFNVISAYY36 pKa = 9.58ILLLVSPSFYY46 pKa = 10.73SLVVGVTMLVGALIRR61 pKa = 11.84LPSGYY66 pKa = 10.52LSDD69 pKa = 3.74SLDD72 pKa = 3.34RR73 pKa = 11.84RR74 pKa = 11.84KK75 pKa = 10.65ALMLTRR81 pKa = 11.84LSQAVLILLPILSLKK96 pKa = 8.45LTPFSYY102 pKa = 10.96VGFNLLTSLNGPLSAGLVQSVMEE125 pKa = 4.13KK126 pKa = 10.11RR127 pKa = 11.84QVLGGTSLNSLTISISSVVGGLVSLSYY154 pKa = 10.34PVLGITAFLSIASVMRR170 pKa = 11.84LFTVFFIGNVRR181 pKa = 11.84VKK183 pKa = 10.63ARR185 pKa = 11.84KK186 pKa = 9.51DD187 pKa = 3.48EE188 pKa = 4.46PKK190 pKa = 10.8GRR192 pKa = 11.84IPVSLIIYY200 pKa = 7.09PILVFSFLDD209 pKa = 3.95VIPVMMNSLVFVVSSVEE226 pKa = 3.64PSLALVSLLTTLGNGMGAILTGEE249 pKa = 4.73RR250 pKa = 11.84IRR252 pKa = 11.84EE253 pKa = 3.78NSIGIWARR261 pKa = 11.84TGSLLLGLVLVAISLSPLYY280 pKa = 10.24LVYY283 pKa = 10.89VLLVLRR289 pKa = 11.84GILSSIQSISIRR301 pKa = 11.84SDD303 pKa = 2.67LRR305 pKa = 11.84LRR307 pKa = 11.84VSNNLMGRR315 pKa = 11.84TWGLITTISSFVASVVALSYY335 pKa = 11.22SFIVSVTGTRR345 pKa = 11.84FPVLVLGLSLLTLGILMVYY364 pKa = 10.01RR365 pKa = 11.84GPSNRR370 pKa = 11.84QDD372 pKa = 3.14TKK374 pKa = 11.64ALGSEE379 pKa = 3.77AGVRR383 pKa = 11.84EE384 pKa = 4.05EE385 pKa = 4.98SSGKK389 pKa = 9.95KK390 pKa = 9.69

Molecular weight: 42.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2256

0

2256

640304

46

1455

283.8

31.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.192 ± 0.052

0.62 ± 0.018

4.704 ± 0.046

6.931 ± 0.063

4.169 ± 0.038

7.477 ± 0.042

1.427 ± 0.019

7.216 ± 0.048

6.072 ± 0.054

10.573 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.023

3.812 ± 0.04

4.359 ± 0.033

2.372 ± 0.029

5.612 ± 0.054

7.099 ± 0.051

5.031 ± 0.051

8.684 ± 0.046

1.045 ± 0.017

3.942 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski