Novosphingobium sp. PP1Y

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4624 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6IMM9|F6IMM9_9SPHN MFS transporter DHA2 family multidrug resistance protein B OS=Novosphingobium sp. PP1Y OX=702113 GN=PP1Y_AT17977 PE=4 SV=1
MM1 pKa = 7.87ASPALAQDD9 pKa = 3.39APGPITVSGEE19 pKa = 3.86VALVSDD25 pKa = 3.59YY26 pKa = 11.1RR27 pKa = 11.84FRR29 pKa = 11.84GVSQTDD35 pKa = 2.87EE36 pKa = 3.97EE37 pKa = 4.7MAVQGGINVEE47 pKa = 4.33HH48 pKa = 7.2EE49 pKa = 4.25SGLYY53 pKa = 10.22AGTWASNLSGWGTFGGSNMEE73 pKa = 4.1LDD75 pKa = 4.78LYY77 pKa = 11.09AGYY80 pKa = 8.5ATEE83 pKa = 4.45IASGLNVDD91 pKa = 5.44VGMTWFMYY99 pKa = 10.31PGGASDD105 pKa = 3.44TDD107 pKa = 3.75FAEE110 pKa = 4.89VYY112 pKa = 10.5VKK114 pKa = 10.89GSTTFGPADD123 pKa = 3.68FLVGVAYY130 pKa = 10.11APKK133 pKa = 9.49QQALGKK139 pKa = 9.63VYY141 pKa = 11.1NNAASYY147 pKa = 10.61NAGIPDD153 pKa = 3.65NPGAKK158 pKa = 9.35SDD160 pKa = 3.92NVYY163 pKa = 9.66IWSDD167 pKa = 3.15ASVGVPNTPLTLSAHH182 pKa = 6.64LGYY185 pKa = 11.21SNGNSGLGPNGTSIAPTGEE204 pKa = 3.85FLDD207 pKa = 3.82WSLGADD213 pKa = 3.75LALGPVTLGVAYY225 pKa = 10.55VDD227 pKa = 3.9TDD229 pKa = 3.25ISSSDD234 pKa = 3.3AAYY237 pKa = 9.55LQPNFASSKK246 pKa = 9.72DD247 pKa = 3.47GSKK250 pKa = 10.23IADD253 pKa = 3.48STILFSISAGFF264 pKa = 3.32

Molecular weight: 27.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6IPJ2|F6IPJ2_9SPHN dITP/XTP pyrophosphatase OS=Novosphingobium sp. PP1Y OX=702113 GN=PP1Y_AT25532 PE=3 SV=1
MM1 pKa = 7.24EE2 pKa = 5.12TGFCPSRR9 pKa = 11.84LRR11 pKa = 11.84KK12 pKa = 7.94PQKK15 pKa = 10.62SEE17 pKa = 3.52ILPFAGTGVSLIPPPGNLSISSFEE41 pKa = 4.48PGWAPRR47 pKa = 11.84CCSRR51 pKa = 11.84SFRR54 pKa = 11.84SVIWPLAVTVRR65 pKa = 11.84LVIGSFLLFRR75 pKa = 11.84HH76 pKa = 5.55NVRR79 pKa = 11.84IYY81 pKa = 10.69NLTVKK86 pKa = 10.52ASSIAAASLCFTAATIAIARR106 pKa = 11.84PVTPDD111 pKa = 3.13DD112 pKa = 3.8AMRR115 pKa = 11.84AAIHH119 pKa = 6.12PVADD123 pKa = 3.97STDD126 pKa = 3.42MQAWLARR133 pKa = 11.84VPVTPDD139 pKa = 3.74FPPDD143 pKa = 3.32FAVTLRR149 pKa = 11.84GEE151 pKa = 4.07APAFVIEE158 pKa = 4.34MSTTPGCLPCADD170 pKa = 4.95LWTKK174 pKa = 10.61LAVLRR179 pKa = 11.84SRR181 pKa = 11.84YY182 pKa = 8.49GWQIRR187 pKa = 11.84TISGEE192 pKa = 3.94EE193 pKa = 3.53AMLRR197 pKa = 11.84SGRR200 pKa = 11.84LGLPWVGHH208 pKa = 4.87PVAWVRR214 pKa = 11.84PVNDD218 pKa = 4.08PNRR221 pKa = 11.84TVPIAIGTDD230 pKa = 3.19HH231 pKa = 7.13AVNLARR237 pKa = 11.84NAYY240 pKa = 9.28LAAKK244 pKa = 8.5MLTGVRR250 pKa = 11.84VDD252 pKa = 3.3VGVRR256 pKa = 11.84ALSKK260 pKa = 9.26FTGIVGLARR269 pKa = 11.84RR270 pKa = 11.84PASIRR275 pKa = 3.44

Molecular weight: 29.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4624

0

4624

1530888

33

2582

331.1

35.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.452 ± 0.047

0.913 ± 0.011

5.91 ± 0.026

5.836 ± 0.034

3.679 ± 0.021

8.798 ± 0.033

2.082 ± 0.017

5.017 ± 0.023

3.119 ± 0.027

9.829 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.018

2.656 ± 0.022

5.2 ± 0.025

3.223 ± 0.02

7.148 ± 0.033

5.663 ± 0.028

5.142 ± 0.025

7.116 ± 0.029

1.443 ± 0.014

2.308 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski