Amino acid dipepetide frequency for Clostridium sp. CAG:632

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.773AlaAla: 6.773 ± 0.113	1.07AlaCys: 1.07 ± 0.046	4.839AlaAsp: 4.839 ± 0.093	6.011AlaGlu: 6.011 ± 0.107	2.85AlaPhe: 2.85 ± 0.069	6.211AlaGly: 6.211 ± 0.097	1.098AlaHis: 1.098 ± 0.042	5.486AlaIle: 5.486 ± 0.098	4.575AlaLys: 4.575 ± 0.098	6.553AlaLeu: 6.553 ± 0.09	2.505AlaMet: 2.505 ± 0.06	2.659AlaAsn: 2.659 ± 0.072	1.99AlaPro: 1.99 ± 0.054	2.419AlaGln: 2.419 ± 0.061	2.85AlaArg: 2.85 ± 0.061	3.836AlaSer: 3.836 ± 0.097	3.679AlaThr: 3.679 ± 0.096	6.028AlaVal: 6.028 ± 0.118	0.613AlaTrp: 0.613 ± 0.033	3.063AlaTyr: 3.063 ± 0.065	0.001AlaXaa: 0.001 ± 0.001
Cys	0.882CysAla: 0.882 ± 0.037	0.26CysCys: 0.26 ± 0.022	0.79CysAsp: 0.79 ± 0.04	0.889CysGlu: 0.889 ± 0.037	0.553CysPhe: 0.553 ± 0.025	1.317CysGly: 1.317 ± 0.048	0.288CysHis: 0.288 ± 0.02	1.161CysIle: 1.161 ± 0.045	0.752CysLys: 0.752 ± 0.033	1.211CysLeu: 1.211 ± 0.044	0.489CysMet: 0.489 ± 0.026	0.572CysAsn: 0.572 ± 0.028	0.591CysPro: 0.591 ± 0.026	0.399CysGln: 0.399 ± 0.025	0.709CysArg: 0.709 ± 0.032	0.836CysSer: 0.836 ± 0.038	0.705CysThr: 0.705 ± 0.032	0.92CysVal: 0.92 ± 0.038	0.111CysTrp: 0.111 ± 0.013	0.613CysTyr: 0.613 ± 0.033	0.001CysXaa: 0.001 ± 0.001
Asp	4.461AspAla: 4.461 ± 0.078	0.74AspCys: 0.74 ± 0.033	2.866AspAsp: 2.866 ± 0.068	4.516AspGlu: 4.516 ± 0.091	2.419AspPhe: 2.419 ± 0.06	4.355AspGly: 4.355 ± 0.097	0.934AspHis: 0.934 ± 0.047	4.578AspIle: 4.578 ± 0.078	3.215AspLys: 3.215 ± 0.065	4.509AspLeu: 4.509 ± 0.085	1.947AspMet: 1.947 ± 0.055	1.983AspAsn: 1.983 ± 0.06	1.75AspPro: 1.75 ± 0.053	1.643AspGln: 1.643 ± 0.052	2.606AspArg: 2.606 ± 0.066	3.217AspSer: 3.217 ± 0.077	3.305AspThr: 3.305 ± 0.081	3.901AspVal: 3.901 ± 0.083	0.555AspTrp: 0.555 ± 0.028	2.978AspTyr: 2.978 ± 0.067	0.0AspXaa: 0.0 ± 0.0
Glu	6.111GluAla: 6.111 ± 0.097	0.84GluCys: 0.84 ± 0.037	4.319GluAsp: 4.319 ± 0.082	7.587GluGlu: 7.587 ± 0.136	2.689GluPhe: 2.689 ± 0.068	4.209GluGly: 4.209 ± 0.072	1.499GluHis: 1.499 ± 0.053	5.934GluIle: 5.934 ± 0.103	5.683GluLys: 5.683 ± 0.103	7.343GluLeu: 7.343 ± 0.117	2.432GluMet: 2.432 ± 0.053	3.96GluAsn: 3.96 ± 0.073	2.095GluPro: 2.095 ± 0.061	3.934GluGln: 3.934 ± 0.095	3.538GluArg: 3.538 ± 0.084	3.374GluSer: 3.374 ± 0.085	4.172GluThr: 4.172 ± 0.084	4.772GluVal: 4.772 ± 0.089	0.625GluTrp: 0.625 ± 0.029	3.369GluTyr: 3.369 ± 0.079	0.0GluXaa: 0.0 ± 0.0
Phe	2.69PheAla: 2.69 ± 0.065	0.686PheCys: 0.686 ± 0.031	2.312PheAsp: 2.312 ± 0.059	2.422PheGlu: 2.422 ± 0.055	1.498PhePhe: 1.498 ± 0.054	2.8PheGly: 2.8 ± 0.077	0.817PheHis: 0.817 ± 0.031	2.596PheIle: 2.596 ± 0.074	1.628PheLys: 1.628 ± 0.049	3.679PheLeu: 3.679 ± 0.089	1.163PheMet: 1.163 ± 0.048	1.371PheAsn: 1.371 ± 0.047	1.211PhePro: 1.211 ± 0.044	1.371PheGln: 1.371 ± 0.046	2.003PheArg: 2.003 ± 0.063	2.358PheSer: 2.358 ± 0.058	2.158PheThr: 2.158 ± 0.062	2.565PheVal: 2.565 ± 0.063	0.415PheTrp: 0.415 ± 0.025	1.539PheTyr: 1.539 ± 0.053	0.001PheXaa: 0.001 ± 0.001
Gly	4.673GlyAla: 4.673 ± 0.101	1.214GlyCys: 1.214 ± 0.041	3.466GlyAsp: 3.466 ± 0.075	4.776GlyGlu: 4.776 ± 0.083	2.85GlyPhe: 2.85 ± 0.074	4.443GlyGly: 4.443 ± 0.088	1.234GlyHis: 1.234 ± 0.042	6.318GlyIle: 6.318 ± 0.107	4.901GlyLys: 4.901 ± 0.079	5.83GlyLeu: 5.83 ± 0.104	2.531GlyMet: 2.531 ± 0.065	3.222GlyAsn: 3.222 ± 0.062	1.24GlyPro: 1.24 ± 0.044	2.319GlyGln: 2.319 ± 0.059	3.04GlyArg: 3.04 ± 0.074	3.983GlySer: 3.983 ± 0.082	4.479GlyThr: 4.479 ± 0.094	4.893GlyVal: 4.893 ± 0.088	0.653GlyTrp: 0.653 ± 0.034	3.416GlyTyr: 3.416 ± 0.073	0.0GlyXaa: 0.0 ± 0.0
His	1.2HisAla: 1.2 ± 0.043	0.308HisCys: 0.308 ± 0.02	0.889HisAsp: 0.889 ± 0.045	1.004HisGlu: 1.004 ± 0.039	0.757HisPhe: 0.757 ± 0.037	1.25HisGly: 1.25 ± 0.042	0.427HisHis: 0.427 ± 0.034	1.395HisIle: 1.395 ± 0.044	0.976HisLys: 0.976 ± 0.041	1.464HisLeu: 1.464 ± 0.048	0.629HisMet: 0.629 ± 0.031	0.739HisAsn: 0.739 ± 0.028	0.914HisPro: 0.914 ± 0.041	0.592HisGln: 0.592 ± 0.028	0.883HisArg: 0.883 ± 0.039	0.959HisSer: 0.959 ± 0.033	1.003HisThr: 1.003 ± 0.037	1.093HisVal: 1.093 ± 0.038	0.15HisTrp: 0.15 ± 0.015	0.786HisTyr: 0.786 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.71IleAla: 5.71 ± 0.098	1.309IleCys: 1.309 ± 0.045	4.077IleAsp: 4.077 ± 0.082	5.202IleGlu: 5.202 ± 0.084	2.746IlePhe: 2.746 ± 0.075	5.322IleGly: 5.322 ± 0.094	1.495IleHis: 1.495 ± 0.054	5.335IleIle: 5.335 ± 0.129	3.817IleLys: 3.817 ± 0.083	7.386IleLeu: 7.386 ± 0.111	2.175IleMet: 2.175 ± 0.064	2.817IleAsn: 2.817 ± 0.07	3.144IlePro: 3.144 ± 0.068	2.824IleGln: 2.824 ± 0.065	4.451IleArg: 4.451 ± 0.093	4.757IleSer: 4.757 ± 0.093	4.336IleThr: 4.336 ± 0.084	5.227IleVal: 5.227 ± 0.099	0.642IleTrp: 0.642 ± 0.034	3.18IleTyr: 3.18 ± 0.075	0.001IleXaa: 0.001 ± 0.001
Lys	4.759LysAla: 4.759 ± 0.093	0.623LysCys: 0.623 ± 0.032	3.672LysAsp: 3.672 ± 0.078	5.853LysGlu: 5.853 ± 0.107	1.559LysPhe: 1.559 ± 0.052	3.663LysGly: 3.663 ± 0.079	1.115LysHis: 1.115 ± 0.036	4.152LysIle: 4.152 ± 0.07	5.138LysLys: 5.138 ± 0.096	5.302LysLeu: 5.302 ± 0.089	1.95LysMet: 1.95 ± 0.056	3.318LysAsn: 3.318 ± 0.064	1.963LysPro: 1.963 ± 0.059	2.877LysGln: 2.877 ± 0.066	2.926LysArg: 2.926 ± 0.073	2.967LysSer: 2.967 ± 0.072	3.568LysThr: 3.568 ± 0.079	3.958LysVal: 3.958 ± 0.088	0.484LysTrp: 0.484 ± 0.029	2.609LysTyr: 2.609 ± 0.065	0.0LysXaa: 0.0 ± 0.0
Leu	6.804LeuAla: 6.804 ± 0.101	1.431LeuCys: 1.431 ± 0.053	5.164LeuAsp: 5.164 ± 0.088	6.488LeuGlu: 6.488 ± 0.113	3.482LeuPhe: 3.482 ± 0.089	5.787LeuGly: 5.787 ± 0.117	1.6LeuHis: 1.6 ± 0.049	6.306LeuIle: 6.306 ± 0.111	5.766LeuLys: 5.766 ± 0.095	8.536LeuLeu: 8.536 ± 0.149	2.605LeuMet: 2.605 ± 0.065	4.047LeuAsn: 4.047 ± 0.074	3.339LeuPro: 3.339 ± 0.071	3.586LeuGln: 3.586 ± 0.079	3.742LeuArg: 3.742 ± 0.083	5.697LeuSer: 5.697 ± 0.091	5.279LeuThr: 5.279 ± 0.1	5.733LeuVal: 5.733 ± 0.09	0.708LeuTrp: 0.708 ± 0.037	3.453LeuTyr: 3.453 ± 0.081	0.004LeuXaa: 0.004 ± 0.002
Met	2.384MetAla: 2.384 ± 0.071	0.331MetCys: 0.331 ± 0.021	1.998MetAsp: 1.998 ± 0.052	2.645MetGlu: 2.645 ± 0.065	0.99MetPhe: 0.99 ± 0.041	1.97MetGly: 1.97 ± 0.051	0.505MetHis: 0.505 ± 0.028	2.358MetIle: 2.358 ± 0.059	2.568MetLys: 2.568 ± 0.064	2.881MetLeu: 2.881 ± 0.065	0.854MetMet: 0.854 ± 0.039	1.702MetAsn: 1.702 ± 0.052	1.103MetPro: 1.103 ± 0.039	1.338MetGln: 1.338 ± 0.054	1.345MetArg: 1.345 ± 0.051	1.716MetSer: 1.716 ± 0.054	1.806MetThr: 1.806 ± 0.045	2.053MetVal: 2.053 ± 0.057	0.201MetTrp: 0.201 ± 0.016	0.95MetTyr: 0.95 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.259AsnAla: 3.259 ± 0.078	0.601AsnCys: 0.601 ± 0.031	2.024AsnAsp: 2.024 ± 0.055	2.887AsnGlu: 2.887 ± 0.08	1.394AsnPhe: 1.394 ± 0.043	3.436AsnGly: 3.436 ± 0.075	0.882AsnHis: 0.882 ± 0.037	3.238AsnIle: 3.238 ± 0.081	2.309AsnLys: 2.309 ± 0.061	3.897AsnLeu: 3.897 ± 0.077	1.444AsnMet: 1.444 ± 0.045	1.8AsnAsn: 1.8 ± 0.058	2.063AsnPro: 2.063 ± 0.057	1.883AsnGln: 1.883 ± 0.063	2.455AsnArg: 2.455 ± 0.066	2.222AsnSer: 2.222 ± 0.061	2.235AsnThr: 2.235 ± 0.068	2.964AsnVal: 2.964 ± 0.076	0.404AsnTrp: 0.404 ± 0.026	2.031AsnTyr: 2.031 ± 0.061	0.001AsnXaa: 0.001 ± 0.001
Pro	2.448ProAla: 2.448 ± 0.059	0.449ProCys: 0.449 ± 0.025	2.324ProAsp: 2.324 ± 0.062	3.724ProGlu: 3.724 ± 0.082	1.324ProPhe: 1.324 ± 0.043	2.284ProGly: 2.284 ± 0.058	0.462ProHis: 0.462 ± 0.025	2.284ProIle: 2.284 ± 0.063	1.775ProLys: 1.775 ± 0.052	2.542ProLeu: 2.542 ± 0.065	0.872ProMet: 0.872 ± 0.042	1.2ProAsn: 1.2 ± 0.046	0.703ProPro: 0.703 ± 0.031	1.011ProGln: 1.011 ± 0.037	0.897ProArg: 0.897 ± 0.038	1.633ProSer: 1.633 ± 0.05	1.586ProThr: 1.586 ± 0.061	3.02ProVal: 3.02 ± 0.07	0.281ProTrp: 0.281 ± 0.018	1.499ProTyr: 1.499 ± 0.045	0.003ProXaa: 0.003 ± 0.002
Gln	3.245GlnAla: 3.245 ± 0.077	0.385GlnCys: 0.385 ± 0.021	1.839GlnAsp: 1.839 ± 0.05	3.355GlnGlu: 3.355 ± 0.08	1.267GlnPhe: 1.267 ± 0.051	2.294GlnGly: 2.294 ± 0.067	0.482GlnHis: 0.482 ± 0.028	3.093GlnIle: 3.093 ± 0.071	2.747GlnLys: 2.747 ± 0.067	3.376GlnLeu: 3.376 ± 0.078	1.349GlnMet: 1.349 ± 0.045	1.823GlnAsn: 1.823 ± 0.05	1.147GlnPro: 1.147 ± 0.045	1.561GlnGln: 1.561 ± 0.062	1.434GlnArg: 1.434 ± 0.048	1.787GlnSer: 1.787 ± 0.051	2.078GlnThr: 2.078 ± 0.063	2.638GlnVal: 2.638 ± 0.07	0.308GlnTrp: 0.308 ± 0.02	1.658GlnTyr: 1.658 ± 0.052	0.0GlnXaa: 0.0 ± 0.0
Arg	2.639ArgAla: 2.639 ± 0.078	0.552ArgCys: 0.552 ± 0.031	2.307ArgAsp: 2.307 ± 0.057	3.958ArgGlu: 3.958 ± 0.093	1.739ArgPhe: 1.739 ± 0.056	2.362ArgGly: 2.362 ± 0.056	0.732ArgHis: 0.732 ± 0.032	4.043ArgIle: 4.043 ± 0.086	3.552ArgLys: 3.552 ± 0.077	4.051ArgLeu: 4.051 ± 0.088	1.699ArgMet: 1.699 ± 0.053	2.299ArgAsn: 2.299 ± 0.06	1.265ArgPro: 1.265 ± 0.045	2.074ArgGln: 2.074 ± 0.062	2.2ArgArg: 2.2 ± 0.056	2.278ArgSer: 2.278 ± 0.051	2.382ArgThr: 2.382 ± 0.059	2.779ArgVal: 2.779 ± 0.063	0.352ArgTrp: 0.352 ± 0.022	1.99ArgTyr: 1.99 ± 0.053	0.001ArgXaa: 0.001 ± 0.001
Ser	3.951SerAla: 3.951 ± 0.082	0.695SerCys: 0.695 ± 0.036	3.225SerAsp: 3.225 ± 0.077	3.899SerGlu: 3.899 ± 0.082	2.299SerPhe: 2.299 ± 0.064	4.888SerGly: 4.888 ± 0.1	0.936SerHis: 0.936 ± 0.039	4.028SerIle: 4.028 ± 0.088	2.967SerLys: 2.967 ± 0.074	4.78SerLeu: 4.78 ± 0.092	1.817SerMet: 1.817 ± 0.054	2.328SerAsn: 2.328 ± 0.065	1.568SerPro: 1.568 ± 0.048	1.779SerGln: 1.779 ± 0.061	2.59SerArg: 2.59 ± 0.063	3.327SerSer: 3.327 ± 0.094	3.058SerThr: 3.058 ± 0.078	4.011SerVal: 4.011 ± 0.071	0.492SerTrp: 0.492 ± 0.021	2.479SerTyr: 2.479 ± 0.065	0.003SerXaa: 0.003 ± 0.002
Thr	4.478ThrAla: 4.478 ± 0.115	0.708ThrCys: 0.708 ± 0.035	3.445ThrAsp: 3.445 ± 0.09	4.53ThrGlu: 4.53 ± 0.088	2.02ThrPhe: 2.02 ± 0.061	5.03ThrGly: 5.03 ± 0.088	0.812ThrHis: 0.812 ± 0.038	4.346ThrIle: 4.346 ± 0.085	2.946ThrLys: 2.946 ± 0.08	4.859ThrLeu: 4.859 ± 0.094	1.549ThrMet: 1.549 ± 0.045	2.131ThrAsn: 2.131 ± 0.069	2.115ThrPro: 2.115 ± 0.055	1.719ThrGln: 1.719 ± 0.054	2.021ThrArg: 2.021 ± 0.057	2.899ThrSer: 2.899 ± 0.073	3.257ThrThr: 3.257 ± 0.097	4.677ThrVal: 4.677 ± 0.103	0.482ThrTrp: 0.482 ± 0.024	2.394ThrTyr: 2.394 ± 0.064	0.003ThrXaa: 0.003 ± 0.002
Val	5.097ValAla: 5.097 ± 0.105	1.084ValCys: 1.084 ± 0.038	3.833ValAsp: 3.833 ± 0.079	4.914ValGlu: 4.914 ± 0.087	2.799ValPhe: 2.799 ± 0.069	4.215ValGly: 4.215 ± 0.096	1.033ValHis: 1.033 ± 0.042	5.518ValIle: 5.518 ± 0.101	4.201ValLys: 4.201 ± 0.074	6.73ValLeu: 6.73 ± 0.097	2.04ValMet: 2.04 ± 0.055	3.083ValAsn: 3.083 ± 0.07	2.446ValPro: 2.446 ± 0.064	2.26ValGln: 2.26 ± 0.058	2.909ValArg: 2.909 ± 0.066	4.351ValSer: 4.351 ± 0.09	4.483ValThr: 4.483 ± 0.108	5.047ValVal: 5.047 ± 0.098	0.615ValTrp: 0.615 ± 0.033	2.857ValTyr: 2.857 ± 0.069	0.0ValXaa: 0.0 ± 0.0
Trp	0.446TrpAla: 0.446 ± 0.025	0.12TrpCys: 0.12 ± 0.015	0.449TrpAsp: 0.449 ± 0.022	0.553TrpGlu: 0.553 ± 0.03	0.368TrpPhe: 0.368 ± 0.024	0.562TrpGly: 0.562 ± 0.031	0.181TrpHis: 0.181 ± 0.015	0.729TrpIle: 0.729 ± 0.035	0.76TrpLys: 0.76 ± 0.034	0.847TrpLeu: 0.847 ± 0.04	0.325TrpMet: 0.325 ± 0.023	0.539TrpAsn: 0.539 ± 0.031	0.158TrpPro: 0.158 ± 0.015	0.394TrpGln: 0.394 ± 0.022	0.349TrpArg: 0.349 ± 0.024	0.461TrpSer: 0.461 ± 0.029	0.385TrpThr: 0.385 ± 0.024	0.501TrpVal: 0.501 ± 0.024	0.087TrpTrp: 0.087 ± 0.011	0.362TrpTyr: 0.362 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.1TyrAla: 3.1 ± 0.066	0.64TyrCys: 0.64 ± 0.038	2.642TyrAsp: 2.642 ± 0.074	3.274TyrGlu: 3.274 ± 0.07	1.68TyrPhe: 1.68 ± 0.055	3.083TyrGly: 3.083 ± 0.076	0.883TyrHis: 0.883 ± 0.039	3.055TyrIle: 3.055 ± 0.065	2.18TyrLys: 2.18 ± 0.056	3.779TyrLeu: 3.779 ± 0.077	1.319TyrMet: 1.319 ± 0.044	1.837TyrAsn: 1.837 ± 0.05	1.513TyrPro: 1.513 ± 0.052	1.88TyrGln: 1.88 ± 0.059	2.308TyrArg: 2.308 ± 0.06	2.458TyrSer: 2.458 ± 0.069	2.463TyrThr: 2.463 ± 0.076	2.732TyrVal: 2.732 ± 0.066	0.401TyrTrp: 0.401 ± 0.029	2.258TyrTyr: 2.258 ± 0.065	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.003XaaCys: 0.003 ± 0.002	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.002	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.007XaaSer: 0.007 ± 0.003	0.0XaaThr: 0.0 ± 0.0	0.003XaaVal: 0.003 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.02XaaXaa: 0.02 ± 0.006

Statistics based on 2230 proteins (701037 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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