Hyposoter fugitivus ichnovirus
Average proteome isoelectric point is 7.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 143 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A2Q0I1|A2Q0I1_9VIRU C17.2 OS=Hyposoter fugitivus ichnovirus OX=265522 PE=4 SV=1
MM1 pKa = 8.04 PEE3 pKa = 3.76 TSEE6 pKa = 4.15 FTTTEE11 pKa = 4.02 PSVSSDD17 pKa = 2.99 SGNTSGSDD25 pKa = 3.06 TKK27 pKa = 11.35 QNGDD31 pKa = 3.32 GDD33 pKa = 4.65 FGTSTVGSEE42 pKa = 4.18 NRR44 pKa = 11.84 LSSYY48 pKa = 10.84 GGSTSSEE55 pKa = 3.89 GMPKK59 pKa = 9.69 TSQSTTTEE67 pKa = 4.11 LSASSDD73 pKa = 3.37 SGNTSEE79 pKa = 6.41 SVTEE83 pKa = 3.97 QNADD87 pKa = 2.86 GDD89 pKa = 4.41 FRR91 pKa = 11.84 TSTVGPEE98 pKa = 4.01 NRR100 pKa = 11.84 LSSYY104 pKa = 10.83 GGSTSSEE111 pKa = 3.89 GMPKK115 pKa = 9.69 TSQSTTTEE123 pKa = 4.11 LSASSDD129 pKa = 3.37 SGNTSEE135 pKa = 6.41 SVTEE139 pKa = 3.97 QNADD143 pKa = 2.86 GDD145 pKa = 4.41 FRR147 pKa = 11.84 TSTVGPEE154 pKa = 4.09 DD155 pKa = 3.6 RR156 pKa = 11.84 LSGYY160 pKa = 9.78 RR161 pKa = 11.84 SSMSSKK167 pKa = 9.48 STSEE171 pKa = 4.32 EE172 pKa = 3.77 NDD174 pKa = 3.23 STTTEE179 pKa = 4.11 VSTSTDD185 pKa = 3.07 SEE187 pKa = 4.28 NASTSDD193 pKa = 3.1 TKK195 pKa = 11.03 QIADD199 pKa = 3.84 EE200 pKa = 4.59 DD201 pKa = 4.69 FGTSTVGTEE210 pKa = 4.09 DD211 pKa = 3.3 HH212 pKa = 6.56 LSGYY216 pKa = 10.28 RR217 pKa = 11.84 SSTSSKK223 pKa = 10.78 GMSEE227 pKa = 4.3 VNASTTTEE235 pKa = 4.04 LSTTTDD241 pKa = 3.18 SEE243 pKa = 4.35 NASTSDD249 pKa = 3.08 AKK251 pKa = 11.08 QNADD255 pKa = 2.88 GDD257 pKa = 4.36 FARR260 pKa = 11.84 STVGSGDD267 pKa = 3.79 HH268 pKa = 5.29 SWEE271 pKa = 4.35 YY272 pKa = 11.18 EE273 pKa = 3.88 DD274 pKa = 4.16 STSSEE279 pKa = 4.27 PVPEE283 pKa = 4.49 LNRR286 pKa = 11.84 TATTRR291 pKa = 11.84 SPTSSDD297 pKa = 3.05 AASAGSLFILYY308 pKa = 8.79 FVVFVCFICICLSTFIPICVYY329 pKa = 10.44 INKK332 pKa = 9.16 KK333 pKa = 10.42 RR334 pKa = 11.84 NFIKK338 pKa = 10.81 NSDD341 pKa = 3.69 MEE343 pKa = 4.39 MKK345 pKa = 9.92 TPHH348 pKa = 6.17 EE349 pKa = 4.48 LCTT352 pKa = 3.78
Molecular weight: 36.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.263
IPC2_protein 4.113
IPC_protein 4.088
Toseland 3.91
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.935
Rodwell 3.923
Grimsley 3.808
Solomon 4.05
Lehninger 3.999
Nozaki 4.151
DTASelect 4.342
Thurlkill 3.923
EMBOSS 3.948
Sillero 4.202
Patrickios 3.592
IPC_peptide 4.05
IPC2_peptide 4.19
IPC2.peptide.svr19 4.12
Protein with the highest isoelectric point:
>tr|A2Q0H3|A2Q0H3_9VIRU N gene-c9.1 OS=Hyposoter fugitivus ichnovirus OX=265522 PE=4 SV=1
MM1 pKa = 8.28 LEE3 pKa = 3.74 ISRR6 pKa = 11.84 LRR8 pKa = 11.84 DD9 pKa = 3.6 KK10 pKa = 10.9 INKK13 pKa = 8.17 GRR15 pKa = 11.84 PRR17 pKa = 11.84 TCLSSACVDD26 pKa = 2.91 IRR28 pKa = 11.84 IYY30 pKa = 11.44 SMSKK34 pKa = 9.47 QGKK37 pKa = 6.33 KK38 pKa = 7.56 QRR40 pKa = 11.84 NILEE44 pKa = 4.42 RR45 pKa = 11.84 DD46 pKa = 3.57 TVSDD50 pKa = 3.69 YY51 pKa = 11.61 VLYY54 pKa = 10.26 IIYY57 pKa = 8.46 EE58 pKa = 4.0 QRR60 pKa = 11.84 HH61 pKa = 4.55 SLRR64 pKa = 11.84 RR65 pKa = 11.84 VLMQKK70 pKa = 9.34 GCRR73 pKa = 11.84 RR74 pKa = 11.84 SVRR77 pKa = 11.84 LPSGTPKK84 pKa = 10.45 KK85 pKa = 9.65 MCRR88 pKa = 11.84 QCTEE92 pKa = 4.22 LLRR95 pKa = 11.84 PDD97 pKa = 4.13 CRR99 pKa = 11.84 TSCSANLTDD108 pKa = 4.42 CLSRR112 pKa = 11.84 TWRR115 pKa = 11.84 SRR117 pKa = 3.04
Molecular weight: 13.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.502
IPC_protein 10.058
Toseland 10.452
ProMoST 10.277
Dawson 10.584
Bjellqvist 10.306
Wikipedia 10.76
Rodwell 10.818
Grimsley 10.628
Solomon 10.657
Lehninger 10.628
Nozaki 10.511
DTASelect 10.262
Thurlkill 10.467
EMBOSS 10.847
Sillero 10.526
Patrickios 10.54
IPC_peptide 10.657
IPC2_peptide 9.663
IPC2.peptide.svr19 8.487
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
143
0
143
26717
67
505
186.8
21.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.936 ± 0.177
3.249 ± 0.127
4.649 ± 0.195
6.314 ± 0.301
5.12 ± 0.208
4.177 ± 0.139
3.526 ± 0.129
5.382 ± 0.197
4.667 ± 0.158
9.649 ± 0.235
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.657 ± 0.088
4.409 ± 0.129
4.439 ± 0.173
3.908 ± 0.163
6.801 ± 0.276
7.939 ± 0.373
5.667 ± 0.264
6.292 ± 0.186
1.4 ± 0.075
3.818 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here