Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) (Arbuscular mycorrhizal fungus) (Glomus intraradices)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Glomeromycotina; Glomeromycetes; Glomerales; Glomeraceae; Rhizophagus; Rhizophagus irregularis

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5TAK6|A0A2H5TAK6_RHIID JEMT01018572.1_cds_EXX66627.1_13402: PROVISIONAL OS=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) OX=747089 GN=RIR_2333520 PE=4 SV=1
MM1 pKa = 7.67SSNQVEE7 pKa = 4.76MNNLMEE13 pKa = 4.36DD14 pKa = 3.42HH15 pKa = 6.78YY16 pKa = 10.54LNSVIPSVASQPIDD30 pKa = 3.36EE31 pKa = 4.89EE32 pKa = 4.52KK33 pKa = 11.22NEE35 pKa = 3.99NEE37 pKa = 4.33DD38 pKa = 3.67TFSLLNLSNVEE49 pKa = 4.42FEE51 pKa = 4.12YY52 pKa = 10.25TYY54 pKa = 11.59DD55 pKa = 3.53NVIDD59 pKa = 3.95VSLMSMNQTRR69 pKa = 11.84MNTLTEE75 pKa = 4.13EE76 pKa = 4.32VHH78 pKa = 6.7CLNNMIPSVASQPIDD93 pKa = 3.27EE94 pKa = 4.58SLEE97 pKa = 3.95YY98 pKa = 10.17TYY100 pKa = 11.33DD101 pKa = 3.37NQFIIEE107 pKa = 4.32NVSLMSMNQVEE118 pKa = 4.9MNNLMEE124 pKa = 4.36DD125 pKa = 3.42HH126 pKa = 6.78YY127 pKa = 10.54LNSVIPSFASQPIDD141 pKa = 3.51EE142 pKa = 4.87KK143 pKa = 11.77NEE145 pKa = 3.87FPLLNLGLLEE155 pKa = 4.11STYY158 pKa = 11.41DD159 pKa = 3.38NQFIIEE165 pKa = 4.91DD166 pKa = 3.96VPLMPMNQATMNNPIEE182 pKa = 4.37EE183 pKa = 4.2DD184 pKa = 3.16HH185 pKa = 6.7FLNGVIPSFVNQPIYY200 pKa = 10.25EE201 pKa = 4.16RR202 pKa = 11.84KK203 pKa = 10.09NKK205 pKa = 9.81IEE207 pKa = 4.57EE208 pKa = 3.99ISPLHH213 pKa = 5.72LTYY216 pKa = 10.97DD217 pKa = 3.48NQFIIEE223 pKa = 4.59DD224 pKa = 3.68VSLMSVASQPIYY236 pKa = 10.22EE237 pKa = 4.68EE238 pKa = 4.2KK239 pKa = 11.09NKK241 pKa = 11.2DD242 pKa = 3.64EE243 pKa = 4.93VNDD246 pKa = 3.8TDD248 pKa = 5.31DD249 pKa = 4.32VIGSKK254 pKa = 9.63MFDD257 pKa = 3.48FVNN260 pKa = 3.46

Molecular weight: 29.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U9SIX8|U9SIX8_RHIID Tis13_13353: PROVISIONAL OS=Rhizophagus irregularis (strain DAOM 181602 / DAOM 197198 / MUCL 43194) OX=747089 GN=GLOINDRAFT_13241 PE=4 SV=1
MM1 pKa = 5.84VTSWLRR7 pKa = 11.84LGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGFFFFLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGLGFRR107 pKa = 11.84VRR109 pKa = 11.84VRR111 pKa = 11.84VRR113 pKa = 11.84VRR115 pKa = 11.84VRR117 pKa = 11.84VRR119 pKa = 11.84VRR121 pKa = 11.84VRR123 pKa = 11.84VRR125 pKa = 11.84VRR127 pKa = 11.84VRR129 pKa = 11.84VRR131 pKa = 11.84VRR133 pKa = 11.84VRR135 pKa = 11.84VRR137 pKa = 11.84GG138 pKa = 3.53

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10641

570

11211

3814083

20

7762

340.2

39.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.518 ± 0.025

1.636 ± 0.013

5.932 ± 0.017

6.868 ± 0.035

4.511 ± 0.017

4.183 ± 0.021

2.306 ± 0.012

8.079 ± 0.026

7.817 ± 0.034

9.064 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.972 ± 0.01

7.15 ± 0.035

4.103 ± 0.023

3.871 ± 0.019

4.738 ± 0.024

7.912 ± 0.043

5.273 ± 0.019

4.669 ± 0.018

1.354 ± 0.01

4.044 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski