Amino acid dipepetide frequency for Mycobacterium phage Clifton

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.0AlaAla: 15.0 ± 1.927
1.171AlaCys: 1.171 ± 0.301
7.472AlaAsp: 7.472 ± 0.558
6.692AlaGlu: 6.692 ± 0.673
2.565AlaPhe: 2.565 ± 0.386
8.755AlaGly: 8.755 ± 1.167
2.677AlaHis: 2.677 ± 0.403
4.517AlaIle: 4.517 ± 0.579
4.071AlaLys: 4.071 ± 0.511
8.588AlaLeu: 8.588 ± 0.751
1.952AlaMet: 1.952 ± 0.273
3.234AlaAsn: 3.234 ± 0.453
5.409AlaPro: 5.409 ± 0.591
3.903AlaGln: 3.903 ± 0.466
7.751AlaArg: 7.751 ± 0.813
4.517AlaSer: 4.517 ± 0.48
6.357AlaThr: 6.357 ± 0.619
6.859AlaVal: 6.859 ± 0.639
2.398AlaTrp: 2.398 ± 0.352
2.231AlaTyr: 2.231 ± 0.348
0.0AlaXaa: 0.0 ± 0.0
Cys
1.115CysAla: 1.115 ± 0.282
0.0CysCys: 0.0 ± 0.0
1.394CysAsp: 1.394 ± 0.319
0.781CysGlu: 0.781 ± 0.195
0.112CysPhe: 0.112 ± 0.08
1.45CysGly: 1.45 ± 0.342
0.446CysHis: 0.446 ± 0.139
0.056CysIle: 0.056 ± 0.061
0.558CysLys: 0.558 ± 0.16
0.892CysLeu: 0.892 ± 0.24
0.167CysMet: 0.167 ± 0.094
0.613CysAsn: 0.613 ± 0.19
1.059CysPro: 1.059 ± 0.306
0.39CysGln: 0.39 ± 0.143
0.669CysArg: 0.669 ± 0.194
0.836CysSer: 0.836 ± 0.267
0.836CysThr: 0.836 ± 0.21
0.613CysVal: 0.613 ± 0.164
0.223CysTrp: 0.223 ± 0.104
0.279CysTyr: 0.279 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
7.138AspAla: 7.138 ± 0.566
1.004AspCys: 1.004 ± 0.26
5.186AspAsp: 5.186 ± 0.539
4.015AspGlu: 4.015 ± 0.475
1.673AspPhe: 1.673 ± 0.249
7.416AspGly: 7.416 ± 0.602
1.506AspHis: 1.506 ± 0.312
2.565AspIle: 2.565 ± 0.364
1.84AspLys: 1.84 ± 0.341
6.413AspLeu: 6.413 ± 0.514
1.283AspMet: 1.283 ± 0.283
1.506AspAsn: 1.506 ± 0.3
5.186AspPro: 5.186 ± 0.539
2.509AspGln: 2.509 ± 0.347
4.796AspArg: 4.796 ± 0.495
3.569AspSer: 3.569 ± 0.579
4.74AspThr: 4.74 ± 0.495
4.405AspVal: 4.405 ± 0.555
1.617AspTrp: 1.617 ± 0.26
1.729AspTyr: 1.729 ± 0.322
0.0AspXaa: 0.0 ± 0.0
Glu
6.469GluAla: 6.469 ± 0.758
0.781GluCys: 0.781 ± 0.26
3.346GluAsp: 3.346 ± 0.387
2.788GluGlu: 2.788 ± 0.478
2.063GluPhe: 2.063 ± 0.379
3.123GluGly: 3.123 ± 0.381
1.338GluHis: 1.338 ± 0.343
2.398GluIle: 2.398 ± 0.389
2.509GluLys: 2.509 ± 0.389
5.799GluLeu: 5.799 ± 0.637
1.45GluMet: 1.45 ± 0.266
1.394GluAsn: 1.394 ± 0.277
2.844GluPro: 2.844 ± 0.567
3.011GluGln: 3.011 ± 0.365
5.409GluArg: 5.409 ± 0.673
3.178GluSer: 3.178 ± 0.463
4.294GluThr: 4.294 ± 0.49
4.126GluVal: 4.126 ± 0.419
1.561GluTrp: 1.561 ± 0.261
1.729GluTyr: 1.729 ± 0.308
0.0GluXaa: 0.0 ± 0.0
Phe
2.509PheAla: 2.509 ± 0.35
0.223PheCys: 0.223 ± 0.115
3.346PheAsp: 3.346 ± 0.581
1.506PheGlu: 1.506 ± 0.258
0.613PhePhe: 0.613 ± 0.169
2.788PheGly: 2.788 ± 0.575
0.613PheHis: 0.613 ± 0.224
1.283PheIle: 1.283 ± 0.333
1.004PheLys: 1.004 ± 0.249
1.673PheLeu: 1.673 ± 0.274
0.836PheMet: 0.836 ± 0.268
1.059PheAsn: 1.059 ± 0.286
1.227PhePro: 1.227 ± 0.278
0.892PheGln: 0.892 ± 0.299
1.784PheArg: 1.784 ± 0.288
1.394PheSer: 1.394 ± 0.288
2.342PheThr: 2.342 ± 0.394
2.454PheVal: 2.454 ± 0.333
0.669PheTrp: 0.669 ± 0.165
0.725PheTyr: 0.725 ± 0.245
0.0PheXaa: 0.0 ± 0.0
Gly
8.086GlyAla: 8.086 ± 1.165
1.171GlyCys: 1.171 ± 0.269
5.911GlyAsp: 5.911 ± 0.567
4.796GlyGlu: 4.796 ± 0.499
2.509GlyPhe: 2.509 ± 0.381
7.584GlyGly: 7.584 ± 1.224
2.119GlyHis: 2.119 ± 0.375
3.625GlyIle: 3.625 ± 0.522
2.398GlyLys: 2.398 ± 0.354
5.521GlyLeu: 5.521 ± 0.517
2.454GlyMet: 2.454 ± 0.429
2.788GlyAsn: 2.788 ± 0.41
3.457GlyPro: 3.457 ± 0.611
2.398GlyGln: 2.398 ± 0.541
6.413GlyArg: 6.413 ± 0.812
4.963GlySer: 4.963 ± 0.549
5.353GlyThr: 5.353 ± 0.553
6.301GlyVal: 6.301 ± 0.606
1.729GlyTrp: 1.729 ± 0.302
2.007GlyTyr: 2.007 ± 0.386
0.0GlyXaa: 0.0 ± 0.0
His
2.454HisAla: 2.454 ± 0.329
0.223HisCys: 0.223 ± 0.114
1.283HisAsp: 1.283 ± 0.238
1.171HisGlu: 1.171 ± 0.247
0.446HisPhe: 0.446 ± 0.126
1.784HisGly: 1.784 ± 0.299
0.892HisHis: 0.892 ± 0.229
1.45HisIle: 1.45 ± 0.308
1.004HisLys: 1.004 ± 0.301
1.952HisLeu: 1.952 ± 0.331
0.502HisMet: 0.502 ± 0.145
1.004HisAsn: 1.004 ± 0.246
1.45HisPro: 1.45 ± 0.293
0.725HisGln: 0.725 ± 0.203
2.509HisArg: 2.509 ± 0.404
0.613HisSer: 0.613 ± 0.183
1.617HisThr: 1.617 ± 0.329
1.617HisVal: 1.617 ± 0.386
0.39HisTrp: 0.39 ± 0.131
0.892HisTyr: 0.892 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
5.074IleAla: 5.074 ± 0.599
0.502IleCys: 0.502 ± 0.203
3.848IleAsp: 3.848 ± 0.561
3.513IleGlu: 3.513 ± 0.344
0.948IlePhe: 0.948 ± 0.222
3.513IleGly: 3.513 ± 0.525
1.283IleHis: 1.283 ± 0.259
1.227IleIle: 1.227 ± 0.272
1.171IleLys: 1.171 ± 0.275
2.119IleLeu: 2.119 ± 0.392
0.502IleMet: 0.502 ± 0.158
1.506IleAsn: 1.506 ± 0.265
2.454IlePro: 2.454 ± 0.338
1.673IleGln: 1.673 ± 0.348
2.9IleArg: 2.9 ± 0.474
1.617IleSer: 1.617 ± 0.319
3.792IleThr: 3.792 ± 0.402
3.346IleVal: 3.346 ± 0.4
1.115IleTrp: 1.115 ± 0.287
0.725IleTyr: 0.725 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
3.848LysAla: 3.848 ± 0.513
0.446LysCys: 0.446 ± 0.148
2.454LysAsp: 2.454 ± 0.351
1.394LysGlu: 1.394 ± 0.275
1.338LysPhe: 1.338 ± 0.213
2.621LysGly: 2.621 ± 0.391
1.283LysHis: 1.283 ± 0.296
1.115LysIle: 1.115 ± 0.224
1.171LysLys: 1.171 ± 0.285
3.067LysLeu: 3.067 ± 0.634
0.502LysMet: 0.502 ± 0.149
1.059LysAsn: 1.059 ± 0.202
2.286LysPro: 2.286 ± 0.436
1.561LysGln: 1.561 ± 0.255
2.231LysArg: 2.231 ± 0.316
1.896LysSer: 1.896 ± 0.297
2.063LysThr: 2.063 ± 0.362
2.621LysVal: 2.621 ± 0.465
0.558LysTrp: 0.558 ± 0.175
1.059LysTyr: 1.059 ± 0.273
0.0LysXaa: 0.0 ± 0.0
Leu
8.086LeuAla: 8.086 ± 0.763
0.892LeuCys: 0.892 ± 0.232
5.409LeuAsp: 5.409 ± 0.584
4.294LeuGlu: 4.294 ± 0.493
2.175LeuPhe: 2.175 ± 0.359
5.855LeuGly: 5.855 ± 0.756
1.171LeuHis: 1.171 ± 0.231
3.178LeuIle: 3.178 ± 0.492
2.063LeuLys: 2.063 ± 0.338
5.13LeuLeu: 5.13 ± 0.678
1.784LeuMet: 1.784 ± 0.325
3.011LeuAsn: 3.011 ± 0.386
5.297LeuPro: 5.297 ± 0.571
3.178LeuGln: 3.178 ± 0.45
5.409LeuArg: 5.409 ± 0.642
5.242LeuSer: 5.242 ± 0.492
6.078LeuThr: 6.078 ± 0.506
5.242LeuVal: 5.242 ± 0.585
1.338LeuTrp: 1.338 ± 0.274
2.286LeuTyr: 2.286 ± 0.429
0.0LeuXaa: 0.0 ± 0.0
Met
2.565MetAla: 2.565 ± 0.389
0.223MetCys: 0.223 ± 0.187
1.506MetAsp: 1.506 ± 0.316
0.781MetGlu: 0.781 ± 0.211
0.558MetPhe: 0.558 ± 0.199
1.394MetGly: 1.394 ± 0.241
0.223MetHis: 0.223 ± 0.094
0.948MetIle: 0.948 ± 0.224
0.558MetLys: 0.558 ± 0.188
1.784MetLeu: 1.784 ± 0.26
0.502MetMet: 0.502 ± 0.204
0.836MetAsn: 0.836 ± 0.177
1.283MetPro: 1.283 ± 0.279
0.502MetGln: 0.502 ± 0.114
1.561MetArg: 1.561 ± 0.297
2.844MetSer: 2.844 ± 0.414
2.398MetThr: 2.398 ± 0.346
1.394MetVal: 1.394 ± 0.327
0.223MetTrp: 0.223 ± 0.117
0.446MetTyr: 0.446 ± 0.15
0.0MetXaa: 0.0 ± 0.0
Asn
3.123AsnAla: 3.123 ± 0.381
0.446AsnCys: 0.446 ± 0.14
1.45AsnAsp: 1.45 ± 0.292
1.561AsnGlu: 1.561 ± 0.345
0.669AsnPhe: 0.669 ± 0.274
3.067AsnGly: 3.067 ± 0.463
1.059AsnHis: 1.059 ± 0.248
1.004AsnIle: 1.004 ± 0.28
0.669AsnLys: 0.669 ± 0.18
3.067AsnLeu: 3.067 ± 0.416
0.892AsnMet: 0.892 ± 0.203
1.784AsnAsn: 1.784 ± 0.4
2.844AsnPro: 2.844 ± 0.344
1.227AsnGln: 1.227 ± 0.332
2.175AsnArg: 2.175 ± 0.29
1.283AsnSer: 1.283 ± 0.23
1.784AsnThr: 1.784 ± 0.253
2.063AsnVal: 2.063 ± 0.384
0.669AsnTrp: 0.669 ± 0.176
0.725AsnTyr: 0.725 ± 0.167
0.0AsnXaa: 0.0 ± 0.0
Pro
5.019ProAla: 5.019 ± 0.604
1.059ProCys: 1.059 ± 0.224
4.517ProAsp: 4.517 ± 0.545
4.405ProGlu: 4.405 ± 0.562
2.119ProPhe: 2.119 ± 0.364
6.19ProGly: 6.19 ± 0.583
1.729ProHis: 1.729 ± 0.312
1.784ProIle: 1.784 ± 0.299
2.454ProLys: 2.454 ± 0.488
4.405ProLeu: 4.405 ± 0.526
1.394ProMet: 1.394 ± 0.341
2.007ProAsn: 2.007 ± 0.313
4.015ProPro: 4.015 ± 0.565
2.509ProGln: 2.509 ± 0.364
3.123ProArg: 3.123 ± 0.547
2.788ProSer: 2.788 ± 0.353
3.29ProThr: 3.29 ± 0.428
4.963ProVal: 4.963 ± 0.446
1.227ProTrp: 1.227 ± 0.257
1.171ProTyr: 1.171 ± 0.25
0.0ProXaa: 0.0 ± 0.0
Gln
4.573GlnAla: 4.573 ± 0.551
0.335GlnCys: 0.335 ± 0.129
1.729GlnAsp: 1.729 ± 0.262
1.784GlnGlu: 1.784 ± 0.3
1.283GlnPhe: 1.283 ± 0.246
2.565GlnGly: 2.565 ± 0.411
1.227GlnHis: 1.227 ± 0.228
2.007GlnIle: 2.007 ± 0.313
1.45GlnLys: 1.45 ± 0.263
3.067GlnLeu: 3.067 ± 0.431
0.558GlnMet: 0.558 ± 0.156
0.613GlnAsn: 0.613 ± 0.195
2.732GlnPro: 2.732 ± 0.403
1.394GlnGln: 1.394 ± 0.279
2.844GlnArg: 2.844 ± 0.49
1.84GlnSer: 1.84 ± 0.33
1.729GlnThr: 1.729 ± 0.28
2.9GlnVal: 2.9 ± 0.391
0.725GlnTrp: 0.725 ± 0.203
0.836GlnTyr: 0.836 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
7.472ArgAla: 7.472 ± 0.665
1.171ArgCys: 1.171 ± 0.301
5.297ArgAsp: 5.297 ± 0.576
4.573ArgGlu: 4.573 ± 0.545
2.621ArgPhe: 2.621 ± 0.386
4.238ArgGly: 4.238 ± 0.556
1.394ArgHis: 1.394 ± 0.29
4.684ArgIle: 4.684 ± 0.463
3.234ArgLys: 3.234 ± 0.446
5.297ArgLeu: 5.297 ± 0.674
2.063ArgMet: 2.063 ± 0.35
2.342ArgAsn: 2.342 ± 0.404
3.736ArgPro: 3.736 ± 0.477
2.063ArgGln: 2.063 ± 0.284
6.58ArgArg: 6.58 ± 0.722
3.68ArgSer: 3.68 ± 0.49
3.457ArgThr: 3.457 ± 0.48
4.851ArgVal: 4.851 ± 0.577
2.063ArgTrp: 2.063 ± 0.361
2.063ArgTyr: 2.063 ± 0.343
0.0ArgXaa: 0.0 ± 0.0
Ser
5.353SerAla: 5.353 ± 0.747
0.446SerCys: 0.446 ± 0.222
3.792SerAsp: 3.792 ± 0.452
2.9SerGlu: 2.9 ± 0.409
2.119SerPhe: 2.119 ± 0.42
4.851SerGly: 4.851 ± 0.478
0.892SerHis: 0.892 ± 0.203
2.175SerIle: 2.175 ± 0.303
2.398SerLys: 2.398 ± 0.348
4.071SerLeu: 4.071 ± 0.483
1.729SerMet: 1.729 ± 0.316
1.896SerAsn: 1.896 ± 0.301
3.346SerPro: 3.346 ± 0.373
1.45SerGln: 1.45 ± 0.285
3.178SerArg: 3.178 ± 0.448
2.732SerSer: 2.732 ± 0.518
3.234SerThr: 3.234 ± 0.407
4.071SerVal: 4.071 ± 0.489
1.059SerTrp: 1.059 ± 0.224
1.45SerTyr: 1.45 ± 0.219
0.0SerXaa: 0.0 ± 0.0
Thr
6.636ThrAla: 6.636 ± 0.666
0.613ThrCys: 0.613 ± 0.178
4.628ThrAsp: 4.628 ± 0.566
4.015ThrGlu: 4.015 ± 0.401
1.617ThrPhe: 1.617 ± 0.232
6.245ThrGly: 6.245 ± 0.504
1.84ThrHis: 1.84 ± 0.317
3.457ThrIle: 3.457 ± 0.437
2.007ThrLys: 2.007 ± 0.354
4.796ThrLeu: 4.796 ± 0.591
1.506ThrMet: 1.506 ± 0.313
1.673ThrAsn: 1.673 ± 0.27
4.963ThrPro: 4.963 ± 0.569
2.007ThrGln: 2.007 ± 0.278
3.848ThrArg: 3.848 ± 0.436
3.569ThrSer: 3.569 ± 0.478
4.74ThrThr: 4.74 ± 0.646
5.576ThrVal: 5.576 ± 0.573
0.948ThrTrp: 0.948 ± 0.232
1.896ThrTyr: 1.896 ± 0.244
0.0ThrXaa: 0.0 ± 0.0
Val
7.416ValAla: 7.416 ± 0.732
1.004ValCys: 1.004 ± 0.201
4.74ValAsp: 4.74 ± 0.51
5.186ValGlu: 5.186 ± 0.618
1.896ValPhe: 1.896 ± 0.349
5.186ValGly: 5.186 ± 0.574
1.283ValHis: 1.283 ± 0.314
3.346ValIle: 3.346 ± 0.438
2.788ValLys: 2.788 ± 0.374
5.409ValLeu: 5.409 ± 0.546
1.506ValMet: 1.506 ± 0.317
1.84ValAsn: 1.84 ± 0.331
4.182ValPro: 4.182 ± 0.419
2.788ValGln: 2.788 ± 0.314
5.13ValArg: 5.13 ± 0.644
4.573ValSer: 4.573 ± 0.591
4.963ValThr: 4.963 ± 0.498
6.134ValVal: 6.134 ± 0.803
1.729ValTrp: 1.729 ± 0.303
1.896ValTyr: 1.896 ± 0.338
0.0ValXaa: 0.0 ± 0.0
Trp
1.896TrpAla: 1.896 ± 0.269
0.279TrpCys: 0.279 ± 0.103
1.171TrpAsp: 1.171 ± 0.306
1.227TrpGlu: 1.227 ± 0.384
0.781TrpPhe: 0.781 ± 0.185
1.115TrpGly: 1.115 ± 0.225
0.669TrpHis: 0.669 ± 0.215
0.948TrpIle: 0.948 ± 0.215
0.669TrpLys: 0.669 ± 0.191
1.896TrpLeu: 1.896 ± 0.369
0.725TrpMet: 0.725 ± 0.205
0.558TrpAsn: 0.558 ± 0.166
0.669TrpPro: 0.669 ± 0.296
1.115TrpGln: 1.115 ± 0.252
2.454TrpArg: 2.454 ± 0.415
1.059TrpSer: 1.059 ± 0.23
1.729TrpThr: 1.729 ± 0.262
1.45TrpVal: 1.45 ± 0.315
0.892TrpTrp: 0.892 ± 0.231
0.39TrpTyr: 0.39 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.286TyrAla: 2.286 ± 0.413
0.502TyrCys: 0.502 ± 0.215
1.673TyrAsp: 1.673 ± 0.387
2.231TyrGlu: 2.231 ± 0.338
0.836TyrPhe: 0.836 ± 0.237
1.84TyrGly: 1.84 ± 0.311
0.335TyrHis: 0.335 ± 0.122
1.059TyrIle: 1.059 ± 0.214
0.558TyrLys: 0.558 ± 0.175
2.175TyrLeu: 2.175 ± 0.389
0.167TyrMet: 0.167 ± 0.089
0.836TyrAsn: 0.836 ± 0.2
1.729TyrPro: 1.729 ± 0.233
0.892TyrGln: 0.892 ± 0.239
2.119TyrArg: 2.119 ± 0.318
0.948TyrSer: 0.948 ± 0.234
1.784TyrThr: 1.784 ± 0.25
2.007TyrVal: 2.007 ± 0.262
0.558TyrTrp: 0.558 ± 0.169
0.39TyrTyr: 0.39 ± 0.134
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (17934 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski