Mycobacterium phage ArcusAngelus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A386KQE0|A0A386KQE0_9CAUD Uncharacterized protein OS=Mycobacterium phage ArcusAngelus OX=2315613 GN=90 PE=4 SV=1
MM1 pKa = 7.44TCLLCDD7 pKa = 3.87HH8 pKa = 7.18PRR10 pKa = 11.84SSHH13 pKa = 6.49APQCRR18 pKa = 11.84VRR20 pKa = 11.84MGVDD24 pKa = 3.13RR25 pKa = 11.84DD26 pKa = 3.78DD27 pKa = 3.68MNTYY31 pKa = 8.02TICLCPGFEE40 pKa = 4.08GTEE43 pKa = 4.07DD44 pKa = 3.76EE45 pKa = 5.48EE46 pKa = 4.89EE47 pKa = 4.23DD48 pKa = 3.61TT49 pKa = 5.47

Molecular weight:
5.58 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A386KQP9|A0A386KQP9_9CAUD Uncharacterized protein OS=Mycobacterium phage ArcusAngelus OX=2315613 GN=37 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.18IHH4 pKa = 5.44VQSRR8 pKa = 11.84GPAGWNATVLFTAGTVYY25 pKa = 10.04TVSDD29 pKa = 3.63DD30 pKa = 3.35QGRR33 pKa = 11.84KK34 pKa = 9.22HH35 pKa = 6.79LIDD38 pKa = 3.4TSRR41 pKa = 11.84VTVRR45 pKa = 11.84RR46 pKa = 11.84LSS48 pKa = 3.31

Molecular weight:
5.33 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

18441

30

1182

179.0

19.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.694 ± 0.436

1.231 ± 0.158

6.719 ± 0.234

5.894 ± 0.296

2.928 ± 0.179

8.573 ± 0.565

2.153 ± 0.196

4.344 ± 0.189

3.205 ± 0.202

7.277 ± 0.217

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.106

3.411 ± 0.142

6.035 ± 0.203

3.433 ± 0.199

7.028 ± 0.407

5.857 ± 0.283

6.659 ± 0.262

7.386 ± 0.266

2.451 ± 0.161

2.484 ± 0.159

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski