Mycobacterium phage ArcusAngelus
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386KQE0|A0A386KQE0_9CAUD Uncharacterized protein OS=Mycobacterium phage ArcusAngelus OX=2315613 GN=90 PE=4 SV=1
MM1 pKa = 7.44 TCLLCDD7 pKa = 3.87 HH8 pKa = 7.18 PRR10 pKa = 11.84 SSHH13 pKa = 6.49 APQCRR18 pKa = 11.84 VRR20 pKa = 11.84 MGVDD24 pKa = 3.13 RR25 pKa = 11.84 DD26 pKa = 3.78 DD27 pKa = 3.68 MNTYY31 pKa = 8.02 TICLCPGFEE40 pKa = 4.08 GTEE43 pKa = 4.07 DD44 pKa = 3.76 EE45 pKa = 5.48 EE46 pKa = 4.89 EE47 pKa = 4.23 DD48 pKa = 3.61 TT49 pKa = 5.47
Molecular weight: 5.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.051
IPC2_protein 4.342
IPC_protein 4.202
Toseland 4.024
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.101
Rodwell 4.037
Grimsley 3.935
Solomon 4.164
Lehninger 4.113
Nozaki 4.304
DTASelect 4.482
Thurlkill 4.075
EMBOSS 4.113
Sillero 4.317
Patrickios 0.846
IPC_peptide 4.164
IPC2_peptide 4.304
IPC2.peptide.svr19 4.205
Protein with the highest isoelectric point:
>tr|A0A386KQP9|A0A386KQP9_9CAUD Uncharacterized protein OS=Mycobacterium phage ArcusAngelus OX=2315613 GN=37 PE=4 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.18 IHH4 pKa = 5.44 VQSRR8 pKa = 11.84 GPAGWNATVLFTAGTVYY25 pKa = 10.04 TVSDD29 pKa = 3.63 DD30 pKa = 3.35 QGRR33 pKa = 11.84 KK34 pKa = 9.22 HH35 pKa = 6.79 LIDD38 pKa = 3.4 TSRR41 pKa = 11.84 VTVRR45 pKa = 11.84 RR46 pKa = 11.84 LSS48 pKa = 3.31
Molecular weight: 5.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.809
IPC_protein 10.906
Toseland 11.023
ProMoST 11.038
Dawson 11.067
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.125
Grimsley 11.111
Solomon 11.345
Lehninger 11.286
Nozaki 10.994
DTASelect 10.877
Thurlkill 11.008
EMBOSS 11.447
Sillero 11.023
Patrickios 10.979
IPC_peptide 11.345
IPC2_peptide 10.014
IPC2.peptide.svr19 8.643
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
103
0
103
18441
30
1182
179.0
19.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.694 ± 0.436
1.231 ± 0.158
6.719 ± 0.234
5.894 ± 0.296
2.928 ± 0.179
8.573 ± 0.565
2.153 ± 0.196
4.344 ± 0.189
3.205 ± 0.202
7.277 ± 0.217
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.24 ± 0.106
3.411 ± 0.142
6.035 ± 0.203
3.433 ± 0.199
7.028 ± 0.407
5.857 ± 0.283
6.659 ± 0.262
7.386 ± 0.266
2.451 ± 0.161
2.484 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here