Lynx canadensis associated microvirus CLP 9413
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CI87|A0A2Z5CI87_9VIRU Replication initiation protein OS=Lynx canadensis associated microvirus CLP 9413 OX=2219141 PE=4 SV=1
MM1 pKa = 7.39 TFGVYY6 pKa = 10.57 AIRR9 pKa = 11.84 DD10 pKa = 3.7 VKK12 pKa = 9.87 TGFMSPHH19 pKa = 7.19 VEE21 pKa = 3.91 EE22 pKa = 5.02 SDD24 pKa = 3.43 PVAIRR29 pKa = 11.84 NFEE32 pKa = 4.26 HH33 pKa = 7.11 AVQSSDD39 pKa = 4.2 GILMSHH45 pKa = 6.93 AADD48 pKa = 4.17 FSFYY52 pKa = 10.88 RR53 pKa = 11.84 LGTFDD58 pKa = 3.96 SATGLIAADD67 pKa = 3.81 TMPTLIMEE75 pKa = 6.05 AIDD78 pKa = 3.82 CVPP81 pKa = 3.47
Molecular weight: 8.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.373
IPC2_protein 4.444
IPC_protein 4.317
Toseland 4.139
ProMoST 4.469
Dawson 4.304
Bjellqvist 4.457
Wikipedia 4.24
Rodwell 4.151
Grimsley 4.05
Solomon 4.291
Lehninger 4.253
Nozaki 4.431
DTASelect 4.635
Thurlkill 4.19
EMBOSS 4.253
Sillero 4.444
Patrickios 3.503
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.345
Protein with the highest isoelectric point:
>tr|A0A2Z5CHX0|A0A2Z5CHX0_9VIRU Nonstructural protein OS=Lynx canadensis associated microvirus CLP 9413 OX=2219141 PE=4 SV=1
MM1 pKa = 7.5 AVNSAANAAQSGVTWQNNPAMSHH24 pKa = 6.0 AVSQASGMIDD34 pKa = 4.0 KK35 pKa = 10.66 ISGIADD41 pKa = 3.72 RR42 pKa = 11.84 NSAWSAAQAEE52 pKa = 4.47 QLRR55 pKa = 11.84 DD56 pKa = 3.13 WQTQQNKK63 pKa = 9.36 IAMDD67 pKa = 3.66 YY68 pKa = 10.63 NAAEE72 pKa = 4.09 AAKK75 pKa = 10.52 NRR77 pKa = 11.84 DD78 pKa = 2.96 WQEE81 pKa = 3.78 YY82 pKa = 7.88 MSNTAHH88 pKa = 5.58 QRR90 pKa = 11.84 EE91 pKa = 4.28 VRR93 pKa = 11.84 DD94 pKa = 3.79 LKK96 pKa = 11.22 AAGLNPILSAMGGNGAAVGSGATASGVTSAGAKK129 pKa = 9.73 GDD131 pKa = 3.66 RR132 pKa = 11.84 DD133 pKa = 3.56 TSANNAIVSVLGSLLNSMTSMAMSTNSAITNLAVADD169 pKa = 4.64 KK170 pKa = 9.78 YY171 pKa = 11.7 NAMSKK176 pKa = 8.76 YY177 pKa = 9.08 TADD180 pKa = 3.77 LSSNTQLAGYY190 pKa = 9.18 RR191 pKa = 11.84 ISAEE195 pKa = 4.14 TALNTANINAAVSRR209 pKa = 11.84 YY210 pKa = 9.99 VSDD213 pKa = 4.06 NNLKK217 pKa = 10.54 GSQAMAAATKK227 pKa = 10.37 ISAQLHH233 pKa = 6.0 ADD235 pKa = 3.32 ASKK238 pKa = 9.72 YY239 pKa = 10.67 AADD242 pKa = 3.88 KK243 pKa = 11.21 GYY245 pKa = 8.79 LTSTDD250 pKa = 3.3 VAKK253 pKa = 10.81 INADD257 pKa = 2.93 INKK260 pKa = 8.89 QLKK263 pKa = 9.76 QMGIDD268 pKa = 3.42 AQFDD272 pKa = 3.6 FARR275 pKa = 11.84 DD276 pKa = 3.64 YY277 pKa = 11.14 PSNAFQAVGSISDD290 pKa = 3.74 LFGNASGGSGKK301 pKa = 10.5 GVSSLFNSVVNGLRR315 pKa = 11.84 GGFGEE320 pKa = 4.41 RR321 pKa = 11.84 KK322 pKa = 9.66 SGFSGGSKK330 pKa = 10.15 GYY332 pKa = 9.6 SGKK335 pKa = 10.44 RR336 pKa = 3.21
Molecular weight: 34.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.675
IPC2_protein 8.653
IPC_protein 8.595
Toseland 9.268
ProMoST 9.151
Dawson 9.545
Bjellqvist 9.224
Wikipedia 9.736
Rodwell 9.794
Grimsley 9.633
Solomon 9.604
Lehninger 9.56
Nozaki 9.209
DTASelect 9.224
Thurlkill 9.37
EMBOSS 9.692
Sillero 9.458
Patrickios 4.825
IPC_peptide 9.604
IPC2_peptide 7.688
IPC2.peptide.svr19 7.686
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1246
81
558
311.5
34.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.433 ± 2.352
0.401 ± 0.341
6.982 ± 0.434
4.173 ± 1.049
3.772 ± 0.606
7.223 ± 0.887
1.605 ± 0.266
4.735 ± 0.474
5.217 ± 1.038
6.742 ± 0.756
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.809 ± 0.537
5.136 ± 1.307
5.056 ± 1.438
3.692 ± 0.51
4.815 ± 0.479
8.668 ± 1.338
6.18 ± 0.556
6.019 ± 1.122
1.525 ± 0.336
4.815 ± 0.933
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here