Pontibacter sp. SGAir0037

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Hymenobacteraceae; Pontibacter; unclassified Pontibacter

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4435 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8RPU6|A0A4P8RPU6_9BACT Alpha/beta hydrolase OS=Pontibacter sp. SGAir0037 OX=2571030 GN=C1N53_15150 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 10.34KK3 pKa = 10.04NWITMVAAAFLCSSMMLTSCGNNTDD28 pKa = 3.54STTGTTEE35 pKa = 3.85GTTSGTGTDD44 pKa = 3.88DD45 pKa = 3.23MGTGTTAGGTYY56 pKa = 10.53EE57 pKa = 5.66DD58 pKa = 5.14DD59 pKa = 3.71TTGTTTTGTTTGMTTGTTTTGTTAGTTTTGTTSGTTAGTTTGTTTTGTTAGTTTSGTTAGTTTGTTTGGTTTGGSTTMM137 pKa = 4.3

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8RMX9|A0A4P8RMX9_9BACT Uncharacterized protein OS=Pontibacter sp. SGAir0037 OX=2571030 GN=C1N53_10685 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.3KK3 pKa = 10.27VSKK6 pKa = 10.6KK7 pKa = 9.73KK8 pKa = 9.45ANKK11 pKa = 8.97RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 8.03PGQRR18 pKa = 11.84NRR20 pKa = 11.84QHH22 pKa = 5.64NWRR25 pKa = 11.84GRR27 pKa = 11.84QPKK30 pKa = 6.75WQRR33 pKa = 11.84RR34 pKa = 11.84LKK36 pKa = 9.8MAVLIAATLLGALAVIAKK54 pKa = 9.26AYY56 pKa = 10.47HH57 pKa = 5.98FFSVHH62 pKa = 5.96LKK64 pKa = 10.24

Molecular weight: 7.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4435

0

4435

1540259

16

2372

347.3

38.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.181 ± 0.049

0.728 ± 0.011

4.95 ± 0.024

6.475 ± 0.039

4.609 ± 0.03

6.874 ± 0.04

2.014 ± 0.019

6.273 ± 0.03

6.067 ± 0.038

9.999 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.015

4.913 ± 0.037

3.992 ± 0.021

4.302 ± 0.028

4.567 ± 0.029

6.151 ± 0.03

5.595 ± 0.037

6.796 ± 0.027

1.157 ± 0.015

4.03 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski