Klebsiella phage vB_KpnS_Domnhall

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Webervirus; Klebsiella virus Domnhall

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NHP0|A0A5B9NHP0_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnS_Domnhall OX=2591369 GN=DOMN_71 PE=4 SV=1
MM1 pKa = 7.73KK2 pKa = 10.29LYY4 pKa = 10.4QDD6 pKa = 3.84QSDD9 pKa = 3.75PAAPMRR15 pKa = 11.84LGSEE19 pKa = 4.19PVSEE23 pKa = 4.13QDD25 pKa = 4.88VIDD28 pKa = 3.92AVINASHH35 pKa = 7.09AEE37 pKa = 4.03NPSALEE43 pKa = 4.1CVTGDD48 pKa = 3.95LLLMSFLPDD57 pKa = 3.21SEE59 pKa = 5.86SYY61 pKa = 9.42IQHH64 pKa = 4.67VTEE67 pKa = 4.63TGQAATRR74 pKa = 11.84PEE76 pKa = 4.27FVGAPNVLQMMLDD89 pKa = 3.39SADD92 pKa = 4.13NIVSLDD98 pKa = 3.6YY99 pKa = 10.75PQQ101 pKa = 5.09

Molecular weight: 10.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NKE6|A0A5B9NKE6_9CAUD Uncharacterized protein OS=Klebsiella phage vB_KpnS_Domnhall OX=2591369 GN=DOMN_60 PE=4 SV=1
MM1 pKa = 7.33NHH3 pKa = 7.24PKK5 pKa = 9.42TDD7 pKa = 3.59SILAVLHH14 pKa = 4.85AHH16 pKa = 5.69GRR18 pKa = 11.84VVLRR22 pKa = 11.84MNRR25 pKa = 11.84EE26 pKa = 3.9SGFTQITITKK36 pKa = 8.84SNGRR40 pKa = 11.84YY41 pKa = 8.81VVGTVPGARR50 pKa = 11.84LIPSSLAGVTLTLEE64 pKa = 4.36SNSMFIEE71 pKa = 4.34SWRR74 pKa = 11.84SS75 pKa = 3.02

Molecular weight: 8.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

90

0

90

16581

39

1150

184.2

20.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.781 ± 0.414

1.393 ± 0.156

6.393 ± 0.199

6.393 ± 0.241

4.24 ± 0.141

7.768 ± 0.227

1.948 ± 0.224

6.049 ± 0.172

6.314 ± 0.329

6.562 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.227 ± 0.154

4.584 ± 0.234

3.311 ± 0.185

3.836 ± 0.27

5.295 ± 0.235

6.182 ± 0.264

5.802 ± 0.259

6.99 ± 0.249

1.574 ± 0.121

3.359 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski