SAR202 cluster bacterium AC-647-N09_OGT_505m

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexi incertae sedis; SAR202 cluster

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 888 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N8YAU8|A0A5N8YAU8_9CHLR Homocysteine S-methyltransferase family protein OS=SAR202 cluster bacterium AC-647-N09_OGT_505m OX=2587833 GN=FIM08_00010 PE=4 SV=1
GG1 pKa = 7.04TGTSLIFKK9 pKa = 9.05YY10 pKa = 10.17QGNPTIRR17 pKa = 11.84NNNFVHH23 pKa = 7.15KK24 pKa = 10.5SEE26 pKa = 4.49TYY28 pKa = 10.31LVYY31 pKa = 10.56DD32 pKa = 4.15DD33 pKa = 5.71RR34 pKa = 11.84NVSEE38 pKa = 4.39NATSDD43 pKa = 4.76FEE45 pKa = 4.87NNWWGTTNTTNLDD58 pKa = 3.31ALIYY62 pKa = 10.22DD63 pKa = 3.96WNDD66 pKa = 2.77NATKK70 pKa = 10.66EE71 pKa = 4.35MIDD74 pKa = 3.43YY75 pKa = 9.35TPFLTAPDD83 pKa = 3.8TTAPPSPPANVATQTGPTTISLAWDD108 pKa = 4.03ANPEE112 pKa = 3.98SDD114 pKa = 2.75ITGYY118 pKa = 10.03KK119 pKa = 8.72VHH121 pKa = 7.08YY122 pKa = 8.35DD123 pKa = 3.27TDD125 pKa = 3.86AAGYY129 pKa = 8.68PYY131 pKa = 10.92ANSIDD136 pKa = 3.59IGNVTSYY143 pKa = 9.93TLSGLSTDD151 pKa = 3.39TTYY154 pKa = 9.94YY155 pKa = 9.4TAVSAYY161 pKa = 10.16DD162 pKa = 3.52ADD164 pKa = 4.9GNEE167 pKa = 4.3SWISSNTTATTEE179 pKa = 4.22SVPTALAFSTQPSGATAGNTFTTQPVVVIQGSAEE213 pKa = 4.13NTVTTATDD221 pKa = 3.26SVTISITSGTGGSGATLLGTATVSAVNGVATFTDD255 pKa = 3.6LRR257 pKa = 11.84IDD259 pKa = 3.55KK260 pKa = 10.46AATGYY265 pKa = 8.62TLTATSSSLTLATSASFDD283 pKa = 3.59VSSSATASRR292 pKa = 11.84LVVISEE298 pKa = 4.44PSTTAAGEE306 pKa = 4.36TFVTQPVIQIQDD318 pKa = 3.34VYY320 pKa = 11.16GNIVTSSSASVTVSITSGTGTSGAALSGSAAISAASGVATFSGLGIDD367 pKa = 4.08SAGSNYY373 pKa = 9.87TLTATASGLTSDD385 pKa = 4.19ASSPFDD391 pKa = 3.35VVVAATPIPAMSTWGLIVMALLVSAWMAHH420 pKa = 4.79MARR423 pKa = 11.84RR424 pKa = 11.84GRR426 pKa = 11.84SWIDD430 pKa = 3.01MKK432 pKa = 11.38

Molecular weight: 44.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N8YDL9|A0A5N8YDL9_9CHLR Molybdenum cofactor biosynthesis protein MoaE OS=SAR202 cluster bacterium AC-647-N09_OGT_505m OX=2587833 GN=FIM08_04185 PE=4 SV=1
MM1 pKa = 7.47PRR3 pKa = 11.84IEE5 pKa = 4.07EE6 pKa = 4.72AIVIPLGPLEE16 pKa = 4.21TWEE19 pKa = 4.34LMRR22 pKa = 11.84DD23 pKa = 3.38LEE25 pKa = 5.48LRR27 pKa = 11.84PRR29 pKa = 11.84WDD31 pKa = 2.98ATIIRR36 pKa = 11.84VGRR39 pKa = 11.84GVVSSDD45 pKa = 3.11AEE47 pKa = 4.22IPRR50 pKa = 11.84LYY52 pKa = 10.95YY53 pKa = 10.37LAPLFLGLRR62 pKa = 11.84WRR64 pKa = 11.84WEE66 pKa = 3.81GEE68 pKa = 4.25YY69 pKa = 10.32ISFQPPNRR77 pKa = 11.84TAVRR81 pKa = 11.84MACGSSLRR89 pKa = 11.84PFKK92 pKa = 11.02SLVGTWVLQSQRR104 pKa = 11.84DD105 pKa = 4.04GTQVRR110 pKa = 11.84MIVSFEE116 pKa = 4.11PRR118 pKa = 11.84WPLPLLGKK126 pKa = 10.33VMGYY130 pKa = 9.78RR131 pKa = 11.84MRR133 pKa = 11.84RR134 pKa = 11.84LLGKK138 pKa = 9.96SLSKK142 pKa = 10.64LRR144 pKa = 11.84AIGTGNHH151 pKa = 6.09SSQDD155 pKa = 3.95SIHH158 pKa = 6.13SS159 pKa = 3.7

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

888

0

888

272155

31

2239

306.5

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.541 ± 0.086

0.888 ± 0.026

5.336 ± 0.062

6.558 ± 0.097

3.687 ± 0.056

8.471 ± 0.079

2.208 ± 0.044

6.066 ± 0.075

3.39 ± 0.06

10.278 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.041

3.196 ± 0.052

4.925 ± 0.052

3.585 ± 0.043

6.176 ± 0.09

6.427 ± 0.065

5.628 ± 0.099

7.73 ± 0.078

1.378 ± 0.037

2.783 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski