Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Haloferax; Haloferax volcanii

Average proteome isoelectric point is 5.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3911 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4GTB9|D4GTB9_HALVD Homolog to carboxylate-amine ligase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=HVO_0744 PE=4 SV=1
MM1 pKa = 7.75SDD3 pKa = 4.14LEE5 pKa = 4.52RR6 pKa = 11.84ICAVGDD12 pKa = 3.78VPDD15 pKa = 4.08DD16 pKa = 3.49TTFLFRR22 pKa = 11.84VRR24 pKa = 11.84AADD27 pKa = 3.82DD28 pKa = 3.68ADD30 pKa = 3.99AEE32 pKa = 4.23EE33 pKa = 5.18LEE35 pKa = 5.04AILVRR40 pKa = 11.84TDD42 pKa = 4.75DD43 pKa = 6.06GIQGWLNYY51 pKa = 9.58CMHH54 pKa = 6.99LTHH57 pKa = 7.02IKK59 pKa = 10.2LDD61 pKa = 3.86KK62 pKa = 11.23GSGAPMRR69 pKa = 11.84NDD71 pKa = 3.07EE72 pKa = 5.3VICQNHH78 pKa = 5.31GAYY81 pKa = 10.36FEE83 pKa = 5.78ADD85 pKa = 2.74TGYY88 pKa = 10.92CNYY91 pKa = 9.67GPCEE95 pKa = 3.94GAVLDD100 pKa = 5.06DD101 pKa = 5.44LDD103 pKa = 3.67ITVDD107 pKa = 3.48GDD109 pKa = 4.25HH110 pKa = 6.74VFLSDD115 pKa = 5.51DD116 pKa = 3.37EE117 pKa = 4.48FDD119 pKa = 3.78YY120 pKa = 11.44VGQGEE125 pKa = 4.79IEE127 pKa = 4.12TDD129 pKa = 3.3PADD132 pKa = 3.97RR133 pKa = 11.84SSSSNVEE140 pKa = 3.96FF141 pKa = 4.89

Molecular weight: 15.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4GQM1|D4GQM1_HALVD Polysaccharide deacetylase domain protein OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=HVO_A0192 PE=4 SV=1
MM1 pKa = 7.21IAKK4 pKa = 9.22PKK6 pKa = 6.23TTPRR10 pKa = 11.84LPDD13 pKa = 3.29HH14 pKa = 6.07STPMNRR20 pKa = 11.84ASVARR25 pKa = 11.84VTRR28 pKa = 11.84LVTVVGAAFVLAGLAVQVTTLRR50 pKa = 11.84LGVPHH55 pKa = 6.63LQWGLRR61 pKa = 11.84AASDD65 pKa = 3.98AEE67 pKa = 4.25VLGVGLWWVAVATRR81 pKa = 11.84LVGPRR86 pKa = 11.84RR87 pKa = 11.84TQRR90 pKa = 11.84RR91 pKa = 11.84VGRR94 pKa = 11.84EE95 pKa = 3.5RR96 pKa = 11.84DD97 pKa = 3.47PTRR100 pKa = 11.84QAVYY104 pKa = 8.2VTRR107 pKa = 11.84GLLDD111 pKa = 3.75ALLGRR116 pKa = 11.84AARR119 pKa = 11.84AEE121 pKa = 3.99PSRR124 pKa = 11.84EE125 pKa = 4.04SLSLGTTAAGEE136 pKa = 3.99LDD138 pKa = 3.6GAEE141 pKa = 4.39SLDD144 pKa = 3.18ATRR147 pKa = 11.84PVFSHH152 pKa = 6.86FVHH155 pKa = 6.91PSAGAAVRR163 pKa = 11.84GVFGVDD169 pKa = 3.55LSTAPGRR176 pKa = 11.84TPGQFVSHH184 pKa = 6.99PWGPLAATKK193 pKa = 10.22RR194 pKa = 11.84DD195 pKa = 3.56TLRR198 pKa = 11.84EE199 pKa = 4.09VVLVAVPPWDD209 pKa = 4.52DD210 pKa = 3.5DD211 pKa = 3.79CVAAFDD217 pKa = 3.8RR218 pKa = 11.84AGRR221 pKa = 11.84RR222 pKa = 11.84RR223 pKa = 11.84PLRR226 pKa = 11.84VVDD229 pKa = 4.89AEE231 pKa = 4.11PCEE234 pKa = 4.49SFDD237 pKa = 4.18EE238 pKa = 5.3AISAAGPAASPPTSSAADD256 pKa = 3.5GG257 pKa = 3.77

Molecular weight: 27.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3911

0

3911

1114463

27

2257

285.0

30.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.09 ± 0.063

0.705 ± 0.013

8.472 ± 0.055

8.0 ± 0.061

3.488 ± 0.03

8.545 ± 0.045

1.966 ± 0.02

3.85 ± 0.032

2.005 ± 0.029

9.05 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.794 ± 0.017

2.382 ± 0.024

4.597 ± 0.025

2.381 ± 0.023

6.61 ± 0.044

5.827 ± 0.033

6.199 ± 0.036

9.236 ± 0.049

1.122 ± 0.015

2.682 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski