Atlantic salmon paramyxovirus (isolate -/Norway/Yrkje371/1995) (ASPV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Aquaparamyxovirus; Salmon aquaparamyxovirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2KSJ3|B2KSJ3_ASPVR Isoform of B2BX74 W protein OS=Atlantic salmon paramyxovirus (isolate -/Norway/Yrkje371/1995) OX=1283346 GN=P/V/C PE=4 SV=1
MM1 pKa = 7.87AEE3 pKa = 3.84QKK5 pKa = 10.56GYY7 pKa = 10.2PNIKK11 pKa = 10.65LNDD14 pKa = 3.4EE15 pKa = 4.45FLNEE19 pKa = 3.94GLNILGCFLRR29 pKa = 11.84GNAQTAPTQEE39 pKa = 4.2AQGHH43 pKa = 5.9KK44 pKa = 10.43GGEE47 pKa = 4.13NDD49 pKa = 3.34TGASQKK55 pKa = 10.92APTPTRR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84NSLSGPSDD71 pKa = 3.47RR72 pKa = 11.84EE73 pKa = 3.72MRR75 pKa = 11.84SQRR78 pKa = 11.84EE79 pKa = 3.68ADD81 pKa = 3.31VDD83 pKa = 3.47KK84 pKa = 10.18WDD86 pKa = 3.45HH87 pKa = 6.23SGVYY91 pKa = 9.27SHH93 pKa = 6.83GLRR96 pKa = 11.84GQLDD100 pKa = 3.38KK101 pKa = 10.93STCEE105 pKa = 3.74EE106 pKa = 4.17FGRR109 pKa = 11.84GQDD112 pKa = 3.63GNTGGLNLCGPDD124 pKa = 3.22VVLNQGPPHH133 pKa = 7.38KK134 pKa = 10.53DD135 pKa = 2.77RR136 pKa = 11.84ALGGIADD143 pKa = 4.33PGKK146 pKa = 9.15KK147 pKa = 7.34THH149 pKa = 7.05LEE151 pKa = 3.95PADD154 pKa = 4.16LEE156 pKa = 4.75DD157 pKa = 4.58GADD160 pKa = 3.67EE161 pKa = 4.29EE162 pKa = 4.69SLPRR166 pKa = 11.84EE167 pKa = 4.09TGGRR171 pKa = 11.84APAGRR176 pKa = 11.84SMEE179 pKa = 4.25GGFTLDD185 pKa = 4.83RR186 pKa = 11.84RR187 pKa = 11.84GSPCKK192 pKa = 9.41PARR195 pKa = 11.84KK196 pKa = 9.41ADD198 pKa = 3.67FRR200 pKa = 11.84EE201 pKa = 4.79GTDD204 pKa = 5.39NPDD207 pKa = 2.72TWSDD211 pKa = 3.23PGTTFMRR218 pKa = 11.84KK219 pKa = 8.53ARR221 pKa = 11.84AMNPQSSASPYY232 pKa = 10.7LEE234 pKa = 4.09EE235 pKa = 3.98QLKK238 pKa = 10.89AEE240 pKa = 4.38KK241 pKa = 10.46AQLLYY246 pKa = 10.46QAEE249 pKa = 4.61DD250 pKa = 3.51NNEE253 pKa = 4.06DD254 pKa = 3.69KK255 pKa = 11.37SEE257 pKa = 4.06GSAPEE262 pKa = 4.11AEE264 pKa = 4.12EE265 pKa = 4.07DD266 pKa = 3.58QEE268 pKa = 4.34EE269 pKa = 4.38EE270 pKa = 4.26EE271 pKa = 4.55EE272 pKa = 3.99EE273 pKa = 4.32DD274 pKa = 5.19EE275 pKa = 4.93IVEE278 pKa = 5.38DD279 pKa = 4.26PDD281 pKa = 3.85SDD283 pKa = 3.72GGEE286 pKa = 4.12TSGLLDD292 pKa = 3.4AHH294 pKa = 6.4NARR297 pKa = 11.84AVDD300 pKa = 3.28AGNRR304 pKa = 11.84IRR306 pKa = 11.84DD307 pKa = 3.35QIYY310 pKa = 9.86DD311 pKa = 3.43VKK313 pKa = 10.41TRR315 pKa = 11.84EE316 pKa = 4.07EE317 pKa = 3.97EE318 pKa = 3.76EE319 pKa = 3.74ARR321 pKa = 11.84QVGKK325 pKa = 10.75KK326 pKa = 9.77GAA328 pKa = 3.54

Molecular weight: 35.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2KSJ2|B2KSJ2_ASPVR Isoform of B2BX74 V protein OS=Atlantic salmon paramyxovirus (isolate -/Norway/Yrkje371/1995) OX=1283346 GN=P/V/C PE=4 SV=1
MM1 pKa = 7.77SSILDD6 pKa = 4.23KK7 pKa = 10.78IKK9 pKa = 10.08RR10 pKa = 11.84WQNKK14 pKa = 8.74RR15 pKa = 11.84ATQTSSSMMSSLTKK29 pKa = 10.32VSTSSGVSSEE39 pKa = 4.38EE40 pKa = 4.09MPKK43 pKa = 10.49LPQPKK48 pKa = 8.89KK49 pKa = 9.86HH50 pKa = 6.62RR51 pKa = 11.84DD52 pKa = 3.15TRR54 pKa = 11.84VVKK57 pKa = 9.07MIPVPLKK64 pKa = 10.56RR65 pKa = 11.84PQLPPGEE72 pKa = 4.28GTVLVVHH79 pKa = 7.05PIGKK83 pKa = 9.46CDD85 pKa = 3.56PRR87 pKa = 11.84EE88 pKa = 4.02KK89 pKa = 10.7RR90 pKa = 11.84MSTSGIIVAYY100 pKa = 9.6IATDD104 pKa = 3.11SGANSIRR111 pKa = 11.84ALVKK115 pKa = 10.35SLEE118 pKa = 4.23EE119 pKa = 4.07VKK121 pKa = 10.11MEE123 pKa = 4.11TPEE126 pKa = 4.47DD127 pKa = 4.08SIFVDD132 pKa = 4.63QMLCSTRR139 pKa = 11.84VLLIRR144 pKa = 11.84IEE146 pKa = 4.07HH147 pKa = 5.43WVEE150 pKa = 3.27LLTRR154 pKa = 11.84GRR156 pKa = 11.84RR157 pKa = 11.84PTWNLLISRR166 pKa = 11.84MALMKK171 pKa = 10.19RR172 pKa = 11.84VSQGKK177 pKa = 8.22PVEE180 pKa = 4.06EE181 pKa = 4.46LLLDD185 pKa = 4.11EE186 pKa = 4.55AWKK189 pKa = 10.85VVLPWIEE196 pKa = 4.18GEE198 pKa = 4.39VRR200 pKa = 11.84ASLPAKK206 pKa = 10.05QISEE210 pKa = 4.31KK211 pKa = 10.88GQTTPTPGQTQEE223 pKa = 3.96RR224 pKa = 11.84LLL226 pKa = 4.04

Molecular weight: 25.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

3

9

5728

226

2209

636.4

70.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.529 ± 0.455

1.92 ± 0.27

5.761 ± 0.497

7.071 ± 0.821

2.968 ± 0.539

7.49 ± 0.697

1.641 ± 0.145

5.901 ± 0.596

5.709 ± 0.41

8.834 ± 0.688

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.898 ± 0.399

4.452 ± 0.376

5.045 ± 0.728

3.352 ± 0.259

5.499 ± 0.401

7.891 ± 0.399

6.494 ± 0.423

6.39 ± 0.595

1.17 ± 0.156

2.985 ± 0.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski