Ruminococcus sp. CAG:724

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1633 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5PWZ6|R5PWZ6_9FIRM MATE efflux family protein OS=Ruminococcus sp. CAG:724 OX=1262966 GN=BN766_00471 PE=4 SV=1
MM1 pKa = 7.26FKK3 pKa = 9.37KK4 pKa = 8.86TKK6 pKa = 10.25LYY8 pKa = 10.61VGVALVVNAVTSLITFIILLFKK30 pKa = 10.6KK31 pKa = 10.05KK32 pKa = 10.29SSAGAFLAVSAMSGAAGAYY51 pKa = 9.98LLYY54 pKa = 10.58DD55 pKa = 4.34CKK57 pKa = 11.36DD58 pKa = 4.42DD59 pKa = 6.28INFCDD64 pKa = 4.55CDD66 pKa = 4.5FNFDD70 pKa = 5.79DD71 pKa = 6.37DD72 pKa = 6.52LDD74 pKa = 4.84DD75 pKa = 6.4CDD77 pKa = 6.11GEE79 pKa = 5.75CDD81 pKa = 4.79DD82 pKa = 5.71CNCDD86 pKa = 3.72DD87 pKa = 6.08CEE89 pKa = 5.66AKK91 pKa = 10.63DD92 pKa = 4.88DD93 pKa = 4.38DD94 pKa = 4.57MNLEE98 pKa = 4.04GDD100 pKa = 3.88LFSRR104 pKa = 11.84EE105 pKa = 4.04DD106 pKa = 3.47EE107 pKa = 4.32EE108 pKa = 4.69

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5PW73|R5PW73_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:724 OX=1262966 GN=BN766_01417 PE=4 SV=1
MM1 pKa = 7.15KK2 pKa = 9.88HH3 pKa = 5.65IAIALVKK10 pKa = 10.03LYY12 pKa = 10.58RR13 pKa = 11.84KK14 pKa = 10.06LISPLKK20 pKa = 9.97RR21 pKa = 11.84PCCRR25 pKa = 11.84FTPTCSQYY33 pKa = 11.17AIEE36 pKa = 5.73AFSEE40 pKa = 3.79WGFIRR45 pKa = 11.84GLGLTIWRR53 pKa = 11.84ILRR56 pKa = 11.84CNPFCRR62 pKa = 11.84GGSDD66 pKa = 3.43PVPKK70 pKa = 10.23RR71 pKa = 11.84KK72 pKa = 9.55RR73 pKa = 3.32

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1633

0

1633

503475

29

3118

308.3

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.131 ± 0.069

1.889 ± 0.031

5.659 ± 0.041

7.03 ± 0.062

4.484 ± 0.043

7.468 ± 0.053

1.663 ± 0.026

7.387 ± 0.061

6.644 ± 0.049

8.493 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.031

3.944 ± 0.052

3.35 ± 0.033

1.888 ± 0.026

4.863 ± 0.06

6.339 ± 0.044

5.59 ± 0.076

6.474 ± 0.053

0.762 ± 0.02

4.12 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski