Amino acid dipepetide frequency for Ruminococcus sp. CAG:724

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.167AlaAla: 10.167 ± 0.217	1.674AlaCys: 1.674 ± 0.068	5.579AlaAsp: 5.579 ± 0.11	7.544AlaGlu: 7.544 ± 0.141	3.825AlaPhe: 3.825 ± 0.105	6.94AlaGly: 6.94 ± 0.132	1.567AlaHis: 1.567 ± 0.055	5.41AlaIle: 5.41 ± 0.116	6.114AlaLys: 6.114 ± 0.115	8.96AlaLeu: 8.96 ± 0.152	2.552AlaMet: 2.552 ± 0.076	2.604AlaAsn: 2.604 ± 0.095	2.558AlaPro: 2.558 ± 0.076	1.939AlaGln: 1.939 ± 0.064	3.798AlaArg: 3.798 ± 0.099	4.967AlaSer: 4.967 ± 0.115	4.026AlaThr: 4.026 ± 0.125	7.412AlaVal: 7.412 ± 0.153	0.526AlaTrp: 0.526 ± 0.033	3.14AlaTyr: 3.14 ± 0.086	0.006AlaXaa: 0.006 ± 0.003
Cys	1.805CysAla: 1.805 ± 0.065	0.264CysCys: 0.264 ± 0.027	1.363CysAsp: 1.363 ± 0.06	1.341CysGlu: 1.341 ± 0.049	0.783CysPhe: 0.783 ± 0.04	2.405CysGly: 2.405 ± 0.098	0.409CysHis: 0.409 ± 0.035	1.154CysIle: 1.154 ± 0.054	0.939CysLys: 0.939 ± 0.047	1.299CysLeu: 1.299 ± 0.053	0.457CysMet: 0.457 ± 0.031	0.59CysAsn: 0.59 ± 0.037	0.705CysPro: 0.705 ± 0.038	0.222CysGln: 0.222 ± 0.02	1.239CysArg: 1.239 ± 0.054	1.11CysSer: 1.11 ± 0.053	1.011CysThr: 1.011 ± 0.059	1.096CysVal: 1.096 ± 0.046	0.125CysTrp: 0.125 ± 0.016	0.57CysTyr: 0.57 ± 0.038	0.0CysXaa: 0.0 ± 0.0
Asp	4.946AspAla: 4.946 ± 0.108	0.916AspCys: 0.916 ± 0.055	3.265AspAsp: 3.265 ± 0.113	4.886AspGlu: 4.886 ± 0.108	3.166AspPhe: 3.166 ± 0.079	5.198AspGly: 5.198 ± 0.118	0.58AspHis: 0.58 ± 0.039	5.5AspIle: 5.5 ± 0.113	3.895AspLys: 3.895 ± 0.089	3.355AspLeu: 3.355 ± 0.082	1.903AspMet: 1.903 ± 0.067	2.018AspAsn: 2.018 ± 0.065	1.589AspPro: 1.589 ± 0.062	0.512AspGln: 0.512 ± 0.032	2.215AspArg: 2.215 ± 0.065	3.039AspSer: 3.039 ± 0.072	3.182AspThr: 3.182 ± 0.088	3.432AspVal: 3.432 ± 0.107	0.445AspTrp: 0.445 ± 0.035	2.552AspTyr: 2.552 ± 0.083	0.0AspXaa: 0.0 ± 0.0
Glu	6.119GluAla: 6.119 ± 0.145	0.918GluCys: 0.918 ± 0.052	2.983GluAsp: 2.983 ± 0.084	5.168GluGlu: 5.168 ± 0.131	2.425GluPhe: 2.425 ± 0.072	3.986GluGly: 3.986 ± 0.094	1.235GluHis: 1.235 ± 0.055	5.502GluIle: 5.502 ± 0.107	7.142GluLys: 7.142 ± 0.133	6.165GluLeu: 6.165 ± 0.113	2.234GluMet: 2.234 ± 0.057	4.534GluAsn: 4.534 ± 0.096	1.76GluPro: 1.76 ± 0.059	1.595GluGln: 1.595 ± 0.053	3.788GluArg: 3.788 ± 0.118	3.849GluSer: 3.849 ± 0.098	3.573GluThr: 3.573 ± 0.087	3.527GluVal: 3.527 ± 0.09	0.534GluTrp: 0.534 ± 0.035	3.255GluTyr: 3.255 ± 0.092	0.0GluXaa: 0.0 ± 0.0
Phe	4.503PheAla: 4.503 ± 0.105	1.073PheCys: 1.073 ± 0.059	3.128PheAsp: 3.128 ± 0.08	2.844PheGlu: 2.844 ± 0.082	2.292PhePhe: 2.292 ± 0.078	3.7PheGly: 3.7 ± 0.105	0.671PheHis: 0.671 ± 0.034	3.283PheIle: 3.283 ± 0.097	1.692PheLys: 1.692 ± 0.064	3.444PheLeu: 3.444 ± 0.09	1.059PheMet: 1.059 ± 0.053	1.372PheAsn: 1.372 ± 0.067	1.535PhePro: 1.535 ± 0.058	0.638PheGln: 0.638 ± 0.035	2.242PheArg: 2.242 ± 0.069	3.674PheSer: 3.674 ± 0.089	2.652PheThr: 2.652 ± 0.089	2.991PheVal: 2.991 ± 0.088	0.334PheTrp: 0.334 ± 0.028	1.708PheTyr: 1.708 ± 0.06	0.006PheXaa: 0.006 ± 0.003
Gly	6.229GlyAla: 6.229 ± 0.123	1.321GlyCys: 1.321 ± 0.053	4.27GlyAsp: 4.27 ± 0.106	5.756GlyGlu: 5.756 ± 0.124	3.319GlyPhe: 3.319 ± 0.094	5.764GlyGly: 5.764 ± 0.142	1.178GlyHis: 1.178 ± 0.05	6.199GlyIle: 6.199 ± 0.099	6.461GlyLys: 6.461 ± 0.128	5.122GlyLeu: 5.122 ± 0.1	2.332GlyMet: 2.332 ± 0.065	3.269GlyAsn: 3.269 ± 0.085	0.936GlyPro: 0.936 ± 0.049	1.289GlyGln: 1.289 ± 0.049	3.355GlyArg: 3.355 ± 0.097	4.284GlySer: 4.284 ± 0.104	4.691GlyThr: 4.691 ± 0.125	4.914GlyVal: 4.914 ± 0.117	0.606GlyTrp: 0.606 ± 0.042	3.379GlyTyr: 3.379 ± 0.089	0.006GlyXaa: 0.006 ± 0.003
His	1.414HisAla: 1.414 ± 0.056	0.34HisCys: 0.34 ± 0.025	1.017HisAsp: 1.017 ± 0.048	1.178HisGlu: 1.178 ± 0.055	0.792HisPhe: 0.792 ± 0.042	1.402HisGly: 1.402 ± 0.057	0.316HisHis: 0.316 ± 0.025	1.511HisIle: 1.511 ± 0.06	0.808HisLys: 0.808 ± 0.043	1.231HisLeu: 1.231 ± 0.048	0.455HisMet: 0.455 ± 0.032	0.665HisAsn: 0.665 ± 0.039	0.785HisPro: 0.785 ± 0.043	0.26HisGln: 0.26 ± 0.022	0.836HisArg: 0.836 ± 0.045	1.009HisSer: 1.009 ± 0.042	0.993HisThr: 0.993 ± 0.052	0.773HisVal: 0.773 ± 0.039	0.141HisTrp: 0.141 ± 0.022	0.705HisTyr: 0.705 ± 0.038	0.0HisXaa: 0.0 ± 0.0
Ile	6.987IleAla: 6.987 ± 0.129	1.75IleCys: 1.75 ± 0.064	4.254IleAsp: 4.254 ± 0.103	4.35IleGlu: 4.35 ± 0.103	3.559IlePhe: 3.559 ± 0.096	5.079IleGly: 5.079 ± 0.119	1.041IleHis: 1.041 ± 0.044	5.408IleIle: 5.408 ± 0.11	4.417IleLys: 4.417 ± 0.087	5.973IleLeu: 5.973 ± 0.115	1.827IleMet: 1.827 ± 0.073	2.894IleAsn: 2.894 ± 0.072	3.17IlePro: 3.17 ± 0.089	1.184IleGln: 1.184 ± 0.048	3.416IleArg: 3.416 ± 0.1	5.73IleSer: 5.73 ± 0.123	4.443IleThr: 4.443 ± 0.118	4.848IleVal: 4.848 ± 0.087	0.546IleTrp: 0.546 ± 0.034	2.989IleTyr: 2.989 ± 0.082	0.0IleXaa: 0.0 ± 0.0
Lys	5.538LysAla: 5.538 ± 0.123	0.959LysCys: 0.959 ± 0.055	2.828LysAsp: 2.828 ± 0.082	4.338LysGlu: 4.338 ± 0.105	2.364LysPhe: 2.364 ± 0.074	3.583LysGly: 3.583 ± 0.094	1.023LysHis: 1.023 ± 0.047	5.295LysIle: 5.295 ± 0.107	6.193LysLys: 6.193 ± 0.136	5.677LysLeu: 5.677 ± 0.098	2.37LysMet: 2.37 ± 0.068	3.772LysAsn: 3.772 ± 0.098	2.115LysPro: 2.115 ± 0.067	1.48LysGln: 1.48 ± 0.055	3.093LysArg: 3.093 ± 0.077	4.107LysSer: 4.107 ± 0.095	4.332LysThr: 4.332 ± 0.095	3.547LysVal: 3.547 ± 0.09	0.522LysTrp: 0.522 ± 0.034	3.309LysTyr: 3.309 ± 0.102	0.0LysXaa: 0.0 ± 0.0
Leu	7.857LeuAla: 7.857 ± 0.136	2.395LeuCys: 2.395 ± 0.076	4.608LeuAsp: 4.608 ± 0.093	4.916LeuGlu: 4.916 ± 0.099	4.042LeuPhe: 4.042 ± 0.114	6.227LeuGly: 6.227 ± 0.13	1.577LeuHis: 1.577 ± 0.06	5.929LeuIle: 5.929 ± 0.116	4.316LeuLys: 4.316 ± 0.101	8.026LeuLeu: 8.026 ± 0.192	2.252LeuMet: 2.252 ± 0.073	2.673LeuAsn: 2.673 ± 0.075	3.674LeuPro: 3.674 ± 0.096	1.392LeuGln: 1.392 ± 0.055	4.681LeuArg: 4.681 ± 0.107	6.928LeuSer: 6.928 ± 0.137	4.683LeuThr: 4.683 ± 0.101	4.815LeuVal: 4.815 ± 0.089	0.733LeuTrp: 0.733 ± 0.046	3.204LeuTyr: 3.204 ± 0.084	0.0LeuXaa: 0.0 ± 0.0
Met	2.395MetAla: 2.395 ± 0.075	0.383MetCys: 0.383 ± 0.028	1.345MetAsp: 1.345 ± 0.056	1.619MetGlu: 1.619 ± 0.061	0.987MetPhe: 0.987 ± 0.051	1.712MetGly: 1.712 ± 0.066	0.463MetHis: 0.463 ± 0.033	1.931MetIle: 1.931 ± 0.064	2.493MetLys: 2.493 ± 0.066	2.975MetLeu: 2.975 ± 0.076	0.765MetMet: 0.765 ± 0.04	1.38MetAsn: 1.38 ± 0.051	1.345MetPro: 1.345 ± 0.049	0.791MetGln: 0.791 ± 0.042	1.68MetArg: 1.68 ± 0.064	1.676MetSer: 1.676 ± 0.052	1.865MetThr: 1.865 ± 0.073	1.333MetVal: 1.333 ± 0.05	0.185MetTrp: 0.185 ± 0.019	0.83MetTyr: 0.83 ± 0.038	0.0MetXaa: 0.0 ± 0.0
Asn	3.583AsnAla: 3.583 ± 0.093	0.642AsnCys: 0.642 ± 0.037	1.956AsnAsp: 1.956 ± 0.069	2.473AsnGlu: 2.473 ± 0.075	1.748AsnPhe: 1.748 ± 0.057	3.563AsnGly: 3.563 ± 0.128	0.622AsnHis: 0.622 ± 0.038	3.619AsnIle: 3.619 ± 0.09	2.163AsnLys: 2.163 ± 0.075	3.083AsnLeu: 3.083 ± 0.078	1.253AsnMet: 1.253 ± 0.051	1.426AsnAsn: 1.426 ± 0.059	1.835AsnPro: 1.835 ± 0.057	0.691AsnGln: 0.691 ± 0.039	1.861AsnArg: 1.861 ± 0.059	2.141AsnSer: 2.141 ± 0.08	2.322AsnThr: 2.322 ± 0.072	2.457AsnVal: 2.457 ± 0.079	0.298AsnTrp: 0.298 ± 0.024	1.698AsnTyr: 1.698 ± 0.067	0.004AsnXaa: 0.004 ± 0.003
Pro	2.999ProAla: 2.999 ± 0.082	0.606ProCys: 0.606 ± 0.037	2.624ProAsp: 2.624 ± 0.075	3.44ProGlu: 3.44 ± 0.086	1.511ProPhe: 1.511 ± 0.06	1.732ProGly: 1.732 ± 0.06	0.679ProHis: 0.679 ± 0.034	2.0ProIle: 2.0 ± 0.059	1.927ProLys: 1.927 ± 0.068	2.763ProLeu: 2.763 ± 0.096	0.894ProMet: 0.894 ± 0.038	1.045ProAsn: 1.045 ± 0.039	0.894ProPro: 0.894 ± 0.043	0.878ProGln: 0.878 ± 0.038	1.343ProArg: 1.343 ± 0.061	1.97ProSer: 1.97 ± 0.065	1.815ProThr: 1.815 ± 0.06	2.626ProVal: 2.626 ± 0.083	0.286ProTrp: 0.286 ± 0.024	1.464ProTyr: 1.464 ± 0.064	0.002ProXaa: 0.002 ± 0.002
Gln	1.567GlnAla: 1.567 ± 0.058	0.304GlnCys: 0.304 ± 0.026	0.749GlnAsp: 0.749 ± 0.034	1.023GlnGlu: 1.023 ± 0.041	0.735GlnPhe: 0.735 ± 0.036	1.158GlnGly: 1.158 ± 0.046	0.302GlnHis: 0.302 ± 0.023	1.575GlnIle: 1.575 ± 0.058	1.67GlnLys: 1.67 ± 0.064	1.639GlnLeu: 1.639 ± 0.055	0.584GlnMet: 0.584 ± 0.04	1.077GlnAsn: 1.077 ± 0.049	0.526GlnPro: 0.526 ± 0.031	0.447GlnGln: 0.447 ± 0.036	0.987GlnArg: 0.987 ± 0.045	1.255GlnSer: 1.255 ± 0.054	1.21GlnThr: 1.21 ± 0.053	1.073GlnVal: 1.073 ± 0.041	0.181GlnTrp: 0.181 ± 0.017	0.822GlnTyr: 0.822 ± 0.043	0.002GlnXaa: 0.002 ± 0.002
Arg	4.036ArgAla: 4.036 ± 0.108	0.719ArgCys: 0.719 ± 0.039	2.814ArgAsp: 2.814 ± 0.083	4.088ArgGlu: 4.088 ± 0.104	2.258ArgPhe: 2.258 ± 0.065	3.293ArgGly: 3.293 ± 0.101	0.953ArgHis: 0.953 ± 0.042	3.941ArgIle: 3.941 ± 0.103	3.208ArgLys: 3.208 ± 0.08	4.423ArgLeu: 4.423 ± 0.118	1.454ArgMet: 1.454 ± 0.055	1.881ArgAsn: 1.881 ± 0.061	1.319ArgPro: 1.319 ± 0.055	1.098ArgGln: 1.098 ± 0.051	3.087ArgArg: 3.087 ± 0.092	2.554ArgSer: 2.554 ± 0.071	2.354ArgThr: 2.354 ± 0.065	2.735ArgVal: 2.735 ± 0.072	0.358ArgTrp: 0.358 ± 0.027	2.056ArgTyr: 2.056 ± 0.071	0.0ArgXaa: 0.0 ± 0.0
Ser	6.312SerAla: 6.312 ± 0.116	1.249SerCys: 1.249 ± 0.05	3.96SerAsp: 3.96 ± 0.082	4.676SerGlu: 4.676 ± 0.103	3.009SerPhe: 3.009 ± 0.082	6.125SerGly: 6.125 ± 0.121	1.106SerHis: 1.106 ± 0.042	3.577SerIle: 3.577 ± 0.087	3.202SerLys: 3.202 ± 0.073	5.732SerLeu: 5.732 ± 0.12	1.559SerMet: 1.559 ± 0.057	1.823SerAsn: 1.823 ± 0.068	2.286SerPro: 2.286 ± 0.075	1.267SerGln: 1.267 ± 0.053	3.096SerArg: 3.096 ± 0.092	3.722SerSer: 3.722 ± 0.098	2.741SerThr: 2.741 ± 0.084	4.922SerVal: 4.922 ± 0.116	0.501SerTrp: 0.501 ± 0.028	2.515SerTyr: 2.515 ± 0.078	0.006SerXaa: 0.006 ± 0.003
Thr	5.722ThrAla: 5.722 ± 0.128	0.814ThrCys: 0.814 ± 0.057	3.47ThrAsp: 3.47 ± 0.09	4.342ThrGlu: 4.342 ± 0.112	2.387ThrPhe: 2.387 ± 0.088	4.523ThrGly: 4.523 ± 0.101	1.015ThrHis: 1.015 ± 0.046	3.202ThrIle: 3.202 ± 0.11	2.89ThrLys: 2.89 ± 0.074	5.234ThrLeu: 5.234 ± 0.109	1.19ThrMet: 1.19 ± 0.052	1.756ThrAsn: 1.756 ± 0.08	2.362ThrPro: 2.362 ± 0.073	1.118ThrGln: 1.118 ± 0.055	2.018ThrArg: 2.018 ± 0.065	2.977ThrSer: 2.977 ± 0.091	3.007ThrThr: 3.007 ± 0.184	5.53ThrVal: 5.53 ± 0.125	0.379ThrTrp: 0.379 ± 0.032	1.96ThrTyr: 1.96 ± 0.082	0.0ThrXaa: 0.0 ± 0.0
Val	5.15ValAla: 5.15 ± 0.109	1.67ValCys: 1.67 ± 0.059	3.188ValAsp: 3.188 ± 0.093	3.178ValGlu: 3.178 ± 0.084	3.341ValPhe: 3.341 ± 0.078	4.435ValGly: 4.435 ± 0.106	0.967ValHis: 0.967 ± 0.044	5.057ValIle: 5.057 ± 0.117	4.147ValLys: 4.147 ± 0.095	5.732ValLeu: 5.732 ± 0.109	1.764ValMet: 1.764 ± 0.065	2.612ValAsn: 2.612 ± 0.076	2.526ValPro: 2.526 ± 0.067	1.104ValGln: 1.104 ± 0.053	3.329ValArg: 3.329 ± 0.084	5.037ValSer: 5.037 ± 0.105	4.018ValThr: 4.018 ± 0.124	4.084ValVal: 4.084 ± 0.11	0.54ValTrp: 0.54 ± 0.037	2.854ValTyr: 2.854 ± 0.072	0.002ValXaa: 0.002 ± 0.002
Trp	0.501TrpAla: 0.501 ± 0.034	0.155TrpCys: 0.155 ± 0.02	0.457TrpAsp: 0.457 ± 0.031	0.491TrpGlu: 0.491 ± 0.031	0.34TrpPhe: 0.34 ± 0.031	0.52TrpGly: 0.52 ± 0.031	0.224TrpHis: 0.224 ± 0.026	0.504TrpIle: 0.504 ± 0.036	0.51TrpLys: 0.51 ± 0.033	0.741TrpLeu: 0.741 ± 0.042	0.215TrpMet: 0.215 ± 0.02	0.411TrpAsn: 0.411 ± 0.032	0.129TrpPro: 0.129 ± 0.016	0.282TrpGln: 0.282 ± 0.024	0.425TrpArg: 0.425 ± 0.033	0.475TrpSer: 0.475 ± 0.036	0.415TrpThr: 0.415 ± 0.032	0.379TrpVal: 0.379 ± 0.031	0.089TrpTrp: 0.089 ± 0.013	0.36TrpTyr: 0.36 ± 0.029	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.47TyrAla: 3.47 ± 0.098	0.737TyrCys: 0.737 ± 0.041	2.731TyrAsp: 2.731 ± 0.091	2.648TyrGlu: 2.648 ± 0.085	1.958TyrPhe: 1.958 ± 0.06	3.325TyrGly: 3.325 ± 0.077	0.703TyrHis: 0.703 ± 0.038	3.269TyrIle: 3.269 ± 0.068	2.256TyrLys: 2.256 ± 0.067	3.355TyrLeu: 3.355 ± 0.084	1.033TyrMet: 1.033 ± 0.048	1.635TyrAsn: 1.635 ± 0.063	1.448TyrPro: 1.448 ± 0.061	0.695TyrGln: 0.695 ± 0.036	2.131TyrArg: 2.131 ± 0.072	2.878TyrSer: 2.878 ± 0.095	2.56TyrThr: 2.56 ± 0.08	2.242TyrVal: 2.242 ± 0.072	0.294TyrTrp: 0.294 ± 0.025	1.825TyrTyr: 1.825 ± 0.072	0.002TyrXaa: 0.002 ± 0.002
Xaa	0.008XaaAla: 0.008 ± 0.004	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.002	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.008XaaGly: 0.008 ± 0.004	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.002	0.002XaaGln: 0.002 ± 0.002	0.008XaaArg: 0.008 ± 0.004	0.002XaaSer: 0.002 ± 0.002	0.002XaaThr: 0.002 ± 0.002	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.044XaaXaa: 0.044 ± 0.013

Statistics based on 1633 proteins (503475 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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