Leptolyngbya sp. NIES-2104

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; Leptolyngbya; unclassified Leptolyngbya

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P4V7M8|A0A0P4V7M8_9CYAN Uncharacterized protein OS=Leptolyngbya sp. NIES-2104 OX=1552121 GN=NIES2104_64880 PE=4 SV=1
MM1 pKa = 7.33IAIDD5 pKa = 3.62SFEE8 pKa = 4.91NGGSWTRR15 pKa = 11.84LRR17 pKa = 11.84DD18 pKa = 4.1FGAYY22 pKa = 8.21EE23 pKa = 3.85QANNPDD29 pKa = 3.6GQNVDD34 pKa = 3.34TNLYY38 pKa = 10.69DD39 pKa = 5.56LLINDD44 pKa = 3.27NTAYY48 pKa = 10.78VVDD51 pKa = 4.88AGANDD56 pKa = 4.56LLSQRR61 pKa = 11.84AFSSEE66 pKa = 4.01VNLEE70 pKa = 3.9AVLPTRR76 pKa = 11.84KK77 pKa = 8.52ATNPFTGEE85 pKa = 4.3AIVQQSVPTATAVGPDD101 pKa = 3.08GALYY105 pKa = 10.58VSEE108 pKa = 4.05LTGFPFQPEE117 pKa = 3.86TAQVYY122 pKa = 10.1RR123 pKa = 11.84INTEE127 pKa = 4.05GQPEE131 pKa = 4.68VYY133 pKa = 10.46AGGSTNIVDD142 pKa = 4.74LAFDD146 pKa = 3.6QSGGLYY152 pKa = 9.89VLEE155 pKa = 4.21YY156 pKa = 10.68DD157 pKa = 5.22ANGLLNEE164 pKa = 4.53GDD166 pKa = 3.92AGALIYY172 pKa = 10.21LSPDD176 pKa = 2.58GQMRR180 pKa = 11.84STITDD185 pKa = 3.62DD186 pKa = 3.82LVSPTGLEE194 pKa = 3.6IGADD198 pKa = 3.2GDD200 pKa = 4.53LYY202 pKa = 11.36VSNKK206 pKa = 9.77GFVAGQGEE214 pKa = 4.63VLRR217 pKa = 11.84LSLEE221 pKa = 4.31GKK223 pKa = 9.76SFNPDD228 pKa = 4.33PICDD232 pKa = 3.45QVKK235 pKa = 10.32YY236 pKa = 8.99YY237 pKa = 7.27TTTISADD244 pKa = 3.26GDD246 pKa = 4.0PADD249 pKa = 3.49VYY251 pKa = 11.62YY252 pKa = 10.14PVLPNSNPDD261 pKa = 3.49QLPIALMLQGALVDD275 pKa = 3.62KK276 pKa = 10.98ADD278 pKa = 3.57YY279 pKa = 10.63SSYY282 pKa = 10.81AEE284 pKa = 4.11EE285 pKa = 3.94VASYY289 pKa = 10.43GFVVVVPNNEE299 pKa = 4.01RR300 pKa = 11.84TLTAPPGSPEE310 pKa = 3.83PYY312 pKa = 8.55ITGFLADD319 pKa = 3.79QQQVTDD325 pKa = 3.6VLAQMRR331 pKa = 11.84IEE333 pKa = 4.34DD334 pKa = 4.29TDD336 pKa = 3.92TNSPISGIVDD346 pKa = 3.64TSKK349 pKa = 11.15LGLLGHH355 pKa = 6.19SFGGYY360 pKa = 10.13AGLASIQDD368 pKa = 3.51INDD371 pKa = 3.87PAVSSGNYY379 pKa = 7.01TRR381 pKa = 11.84PSEE384 pKa = 4.02LKK386 pKa = 10.35AGIFYY391 pKa = 7.65GTNFQTPPSSRR402 pKa = 11.84VFPPIDD408 pKa = 3.6NQDD411 pKa = 3.28IPVGLIAGTLDD422 pKa = 3.24GVADD426 pKa = 4.33LGEE429 pKa = 4.16VASTYY434 pKa = 11.17VKK436 pKa = 10.89VQDD439 pKa = 4.07PSKK442 pKa = 11.3ALITVEE448 pKa = 4.13GANHH452 pKa = 6.15YY453 pKa = 10.91GITNQDD459 pKa = 2.96SDD461 pKa = 4.33RR462 pKa = 11.84DD463 pKa = 4.41SVCPTLDD470 pKa = 3.02QATANGATLAVSASQRR486 pKa = 11.84GRR488 pKa = 11.84WSGLFLRR495 pKa = 11.84EE496 pKa = 3.92LRR498 pKa = 11.84CTGTCSHH505 pKa = 7.19LLGDD509 pKa = 4.96PGAFDD514 pKa = 3.85YY515 pKa = 11.19VYY517 pKa = 9.77KK518 pKa = 10.61TGGDD522 pKa = 3.75LNPTVNVTSQTSGII536 pKa = 3.94

Molecular weight: 56.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P4UTS3|A0A0P4UTS3_9CYAN Uncharacterized protein OS=Leptolyngbya sp. NIES-2104 OX=1552121 GN=NIES2104_23520 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.01RR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 8.87RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84VSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.4NGQKK30 pKa = 10.06VIQARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.2KK38 pKa = 10.15GRR40 pKa = 11.84LRR42 pKa = 11.84LSVSSSS48 pKa = 2.84

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6684

0

6684

1864961

37

4762

279.0

31.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.75 ± 0.03

0.935 ± 0.012

5.182 ± 0.031

6.202 ± 0.036

4.023 ± 0.024

6.635 ± 0.037

1.827 ± 0.018

6.436 ± 0.023

3.923 ± 0.026

10.781 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.959 ± 0.015

3.735 ± 0.025

4.797 ± 0.023

5.478 ± 0.029

6.043 ± 0.024

6.653 ± 0.028

5.731 ± 0.035

6.728 ± 0.028

1.442 ± 0.013

2.741 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski