Cajanus cajan (Pigeon pea) (Cajanus indicus)
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47180 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A151S288|A0A151S288_CAJCA F-box protein At2g32560 family OS=Cajanus cajan OX=3821 GN=KK1_029324 PE=4 SV=1
MM1 pKa = 7.65 GSQVCEE7 pKa = 3.58 TLAYY11 pKa = 9.81 INGDD15 pKa = 3.23 EE16 pKa = 4.55 GVRR19 pKa = 11.84 GMEE22 pKa = 3.83 ALQINGALLMSLMEE36 pKa = 4.5 EE37 pKa = 4.41 SSPSDD42 pKa = 3.2 NHH44 pKa = 7.34 DD45 pKa = 3.37 EE46 pKa = 4.52 SDD48 pKa = 4.45 DD49 pKa = 3.93 DD50 pKa = 5.34 RR51 pKa = 11.84 LDD53 pKa = 3.32 SLIRR57 pKa = 11.84 SFEE60 pKa = 4.07 AEE62 pKa = 3.36 ISGSNKK68 pKa = 9.26 MGGDD72 pKa = 3.62 HH73 pKa = 7.38 DD74 pKa = 5.38 HH75 pKa = 7.57 DD76 pKa = 4.47 LASKK80 pKa = 10.64 GSEE83 pKa = 3.71 FVSKK87 pKa = 10.43 MEE89 pKa = 3.95 EE90 pKa = 4.3 CANQTWNIMGDD101 pKa = 3.5 VDD103 pKa = 4.26 GVEE106 pKa = 4.41 YY107 pKa = 9.88 WGSSSSEE114 pKa = 4.17 FGVEE118 pKa = 3.81 WVDD121 pKa = 3.19 MDD123 pKa = 5.26 LVPLEE128 pKa = 5.38 DD129 pKa = 3.58 RR130 pKa = 11.84 SNWCVDD136 pKa = 3.33 PCGDD140 pKa = 4.09 EE141 pKa = 3.91 YY142 pKa = 12.11 AMANNFKK149 pKa = 10.84 VYY151 pKa = 10.71 DD152 pKa = 3.82 GFALGEE158 pKa = 4.0 HH159 pKa = 7.48 GYY161 pKa = 11.03 NSLWQDD167 pKa = 3.27 MACNN171 pKa = 3.66
Molecular weight: 18.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 1.1
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A151SQY2|A0A151SQY2_CAJCA Homoserine_dh domain-containing protein OS=Cajanus cajan OX=3821 GN=KK1_003400 PE=4 SV=1
MM1 pKa = 7.68 AGAPFLPIRR10 pKa = 11.84 TPPGAKK16 pKa = 10.0 ALLSPKK22 pKa = 9.3 TAFLLTVMWQRR33 pKa = 11.84 SQSFSTLLPVRR44 pKa = 11.84 PRR46 pKa = 11.84 GRR48 pKa = 11.84 RR49 pKa = 11.84 SHH51 pKa = 5.6 KK52 pKa = 9.48 TRR54 pKa = 3.42
Molecular weight: 6.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.471
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.29
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.457
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47180
0
47180
15280909
49
5241
323.9
36.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.22 ± 0.011
1.975 ± 0.005
5.136 ± 0.009
6.196 ± 0.011
4.506 ± 0.008
6.046 ± 0.011
2.679 ± 0.006
5.626 ± 0.008
6.527 ± 0.013
9.947 ± 0.014
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.452 ± 0.005
4.575 ± 0.008
4.709 ± 0.013
3.767 ± 0.007
5.217 ± 0.008
8.334 ± 0.014
4.948 ± 0.008
6.556 ± 0.008
1.452 ± 0.005
3.01 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here