Amino acid dipepetide frequency for Mycolicibacterium holsaticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.464AlaAla: 19.464 ± 0.14	0.999AlaCys: 0.999 ± 0.026	8.581AlaAsp: 8.581 ± 0.069	7.921AlaGlu: 7.921 ± 0.087	3.473AlaPhe: 3.473 ± 0.052	11.257AlaGly: 11.257 ± 0.097	2.549AlaHis: 2.549 ± 0.041	5.095AlaIle: 5.095 ± 0.062	3.13AlaLys: 3.13 ± 0.051	12.69AlaLeu: 12.69 ± 0.096	2.94AlaMet: 2.94 ± 0.039	2.708AlaAsn: 2.708 ± 0.044	6.363AlaPro: 6.363 ± 0.076	4.104AlaGln: 4.104 ± 0.051	8.401AlaArg: 8.401 ± 0.094	5.804AlaSer: 5.804 ± 0.067	7.164AlaThr: 7.164 ± 0.064	11.89AlaVal: 11.89 ± 0.104	1.606AlaTrp: 1.606 ± 0.036	2.411AlaTyr: 2.411 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.131CysAla: 1.131 ± 0.03	0.087CysCys: 0.087 ± 0.008	0.576CysAsp: 0.576 ± 0.019	0.416CysGlu: 0.416 ± 0.017	0.241CysPhe: 0.241 ± 0.013	0.974CysGly: 0.974 ± 0.028	0.178CysHis: 0.178 ± 0.011	0.279CysIle: 0.279 ± 0.013	0.129CysLys: 0.129 ± 0.009	0.687CysLeu: 0.687 ± 0.019	0.145CysMet: 0.145 ± 0.01	0.168CysAsn: 0.168 ± 0.01	0.493CysPro: 0.493 ± 0.017	0.237CysGln: 0.237 ± 0.012	0.606CysArg: 0.606 ± 0.021	0.466CysSer: 0.466 ± 0.016	0.495CysThr: 0.495 ± 0.019	0.731CysVal: 0.731 ± 0.023	0.149CysTrp: 0.149 ± 0.009	0.215CysTyr: 0.215 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	8.032AspAla: 8.032 ± 0.085	0.466AspCys: 0.466 ± 0.016	4.655AspAsp: 4.655 ± 0.06	4.221AspGlu: 4.221 ± 0.065	1.864AspPhe: 1.864 ± 0.039	6.092AspGly: 6.092 ± 0.068	1.369AspHis: 1.369 ± 0.03	2.732AspIle: 2.732 ± 0.037	1.374AspLys: 1.374 ± 0.03	5.926AspLeu: 5.926 ± 0.058	1.082AspMet: 1.082 ± 0.027	1.316AspAsn: 1.316 ± 0.03	4.51AspPro: 4.51 ± 0.054	1.807AspGln: 1.807 ± 0.035	4.596AspArg: 4.596 ± 0.058	2.74AspSer: 2.74 ± 0.043	3.335AspThr: 3.335 ± 0.05	5.782AspVal: 5.782 ± 0.066	1.054AspTrp: 1.054 ± 0.029	1.486AspTyr: 1.486 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.047GluAla: 6.047 ± 0.071	0.38GluCys: 0.38 ± 0.014	2.66GluAsp: 2.66 ± 0.05	2.597GluGlu: 2.597 ± 0.044	1.959GluPhe: 1.959 ± 0.035	3.375GluGly: 3.375 ± 0.049	1.596GluHis: 1.596 ± 0.035	2.617GluIle: 2.617 ± 0.041	1.425GluLys: 1.425 ± 0.032	6.614GluLeu: 6.614 ± 0.076	1.147GluMet: 1.147 ± 0.025	1.176GluAsn: 1.176 ± 0.029	3.192GluPro: 3.192 ± 0.046	2.339GluGln: 2.339 ± 0.041	4.481GluArg: 4.481 ± 0.059	2.594GluSer: 2.594 ± 0.043	2.777GluThr: 2.777 ± 0.044	4.414GluVal: 4.414 ± 0.055	0.782GluTrp: 0.782 ± 0.024	1.177GluTyr: 1.177 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	3.983PheAla: 3.983 ± 0.055	0.341PheCys: 0.341 ± 0.015	2.553PheAsp: 2.553 ± 0.041	1.622PheGlu: 1.622 ± 0.032	1.022PhePhe: 1.022 ± 0.033	3.538PheGly: 3.538 ± 0.046	0.639PheHis: 0.639 ± 0.019	1.156PheIle: 1.156 ± 0.026	0.543PheLys: 0.543 ± 0.02	2.593PheLeu: 2.593 ± 0.042	0.494PheMet: 0.494 ± 0.019	0.808PheAsn: 0.808 ± 0.025	1.425PhePro: 1.425 ± 0.033	0.701PheGln: 0.701 ± 0.024	1.778PheArg: 1.778 ± 0.035	1.737PheSer: 1.737 ± 0.034	2.19PheThr: 2.19 ± 0.038	2.625PheVal: 2.625 ± 0.042	0.491PheTrp: 0.491 ± 0.018	0.738PheTyr: 0.738 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	10.108GlyAla: 10.108 ± 0.09	0.805GlyCys: 0.805 ± 0.02	5.051GlyAsp: 5.051 ± 0.069	4.372GlyGlu: 4.372 ± 0.054	3.071GlyPhe: 3.071 ± 0.045	7.635GlyGly: 7.635 ± 0.116	1.981GlyHis: 1.981 ± 0.037	3.978GlyIle: 3.978 ± 0.05	2.273GlyLys: 2.273 ± 0.036	8.386GlyLeu: 8.386 ± 0.08	2.159GlyMet: 2.159 ± 0.038	1.923GlyAsn: 1.923 ± 0.044	4.56GlyPro: 4.56 ± 0.063	2.948GlyGln: 2.948 ± 0.047	6.206GlyArg: 6.206 ± 0.068	4.913GlySer: 4.913 ± 0.053	5.119GlyThr: 5.119 ± 0.059	7.641GlyVal: 7.641 ± 0.078	1.628GlyTrp: 1.628 ± 0.033	2.394GlyTyr: 2.394 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.386HisAla: 2.386 ± 0.043	0.217HisCys: 0.217 ± 0.012	1.363HisAsp: 1.363 ± 0.03	1.051HisGlu: 1.051 ± 0.026	0.724HisPhe: 0.724 ± 0.021	2.015HisGly: 2.015 ± 0.034	0.66HisHis: 0.66 ± 0.021	0.882HisIle: 0.882 ± 0.022	0.376HisLys: 0.376 ± 0.015	2.093HisLeu: 2.093 ± 0.038	0.375HisMet: 0.375 ± 0.014	0.478HisAsn: 0.478 ± 0.015	1.662HisPro: 1.662 ± 0.03	0.666HisGln: 0.666 ± 0.02	1.884HisArg: 1.884 ± 0.035	1.059HisSer: 1.059 ± 0.024	1.221HisThr: 1.221 ± 0.029	1.727HisVal: 1.727 ± 0.031	0.375HisTrp: 0.375 ± 0.017	0.56HisTyr: 0.56 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.308IleAla: 6.308 ± 0.059	0.435IleCys: 0.435 ± 0.014	3.503IleAsp: 3.503 ± 0.053	2.673IleGlu: 2.673 ± 0.041	1.042IlePhe: 1.042 ± 0.029	4.451IleGly: 4.451 ± 0.055	0.816IleHis: 0.816 ± 0.019	1.5IleIle: 1.5 ± 0.033	0.925IleLys: 0.925 ± 0.026	2.935IleLeu: 2.935 ± 0.041	0.619IleMet: 0.619 ± 0.021	1.178IleAsn: 1.178 ± 0.027	2.382IlePro: 2.382 ± 0.035	0.976IleGln: 0.976 ± 0.024	2.595IleArg: 2.595 ± 0.036	2.239IleSer: 2.239 ± 0.04	2.882IleThr: 2.882 ± 0.045	3.788IleVal: 3.788 ± 0.052	0.482IleTrp: 0.482 ± 0.015	0.804IleTyr: 0.804 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	2.778LysAla: 2.778 ± 0.05	0.139LysCys: 0.139 ± 0.01	1.183LysAsp: 1.183 ± 0.029	1.004LysGlu: 1.004 ± 0.027	0.667LysPhe: 0.667 ± 0.02	1.609LysGly: 1.609 ± 0.039	0.515LysHis: 0.515 ± 0.017	1.036LysIle: 1.036 ± 0.026	0.666LysLys: 0.666 ± 0.023	2.286LysLeu: 2.286 ± 0.042	0.532LysMet: 0.532 ± 0.02	0.548LysAsn: 0.548 ± 0.019	1.486LysPro: 1.486 ± 0.032	0.762LysGln: 0.762 ± 0.023	1.665LysArg: 1.665 ± 0.031	1.204LysSer: 1.204 ± 0.029	1.398LysThr: 1.398 ± 0.027	2.012LysVal: 2.012 ± 0.044	0.339LysTrp: 0.339 ± 0.015	0.497LysTyr: 0.497 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	13.679LeuAla: 13.679 ± 0.104	0.873LeuCys: 0.873 ± 0.022	6.544LeuAsp: 6.544 ± 0.078	4.403LeuGlu: 4.403 ± 0.068	2.686LeuPhe: 2.686 ± 0.044	8.531LeuGly: 8.531 ± 0.073	2.0LeuHis: 2.0 ± 0.032	4.114LeuIle: 4.114 ± 0.053	1.902LeuLys: 1.902 ± 0.043	9.411LeuLeu: 9.411 ± 0.106	1.793LeuMet: 1.793 ± 0.032	2.288LeuAsn: 2.288 ± 0.039	5.627LeuPro: 5.627 ± 0.063	2.643LeuGln: 2.643 ± 0.041	7.512LeuArg: 7.512 ± 0.079	5.48LeuSer: 5.48 ± 0.058	6.244LeuThr: 6.244 ± 0.079	8.266LeuVal: 8.266 ± 0.082	1.201LeuTrp: 1.201 ± 0.031	1.713LeuTyr: 1.713 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.708MetAla: 2.708 ± 0.042	0.214MetCys: 0.214 ± 0.012	0.922MetAsp: 0.922 ± 0.021	0.76MetGlu: 0.76 ± 0.024	0.696MetPhe: 0.696 ± 0.019	1.558MetGly: 1.558 ± 0.035	0.467MetHis: 0.467 ± 0.017	0.886MetIle: 0.886 ± 0.021	0.503MetLys: 0.503 ± 0.019	2.164MetLeu: 2.164 ± 0.034	0.469MetMet: 0.469 ± 0.019	0.519MetAsn: 0.519 ± 0.017	1.279MetPro: 1.279 ± 0.027	0.61MetGln: 0.61 ± 0.02	1.65MetArg: 1.65 ± 0.029	1.571MetSer: 1.571 ± 0.026	1.935MetThr: 1.935 ± 0.032	1.693MetVal: 1.693 ± 0.032	0.299MetTrp: 0.299 ± 0.012	0.362MetTyr: 0.362 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.769AsnAla: 2.769 ± 0.037	0.206AsnCys: 0.206 ± 0.011	1.284AsnAsp: 1.284 ± 0.033	1.104AsnGlu: 1.104 ± 0.028	0.718AsnPhe: 0.718 ± 0.023	2.147AsnGly: 2.147 ± 0.042	0.475AsnHis: 0.475 ± 0.016	0.981AsnIle: 0.981 ± 0.025	0.472AsnLys: 0.472 ± 0.017	2.162AsnLeu: 2.162 ± 0.041	0.456AsnMet: 0.456 ± 0.016	0.608AsnAsn: 0.608 ± 0.021	1.818AsnPro: 1.818 ± 0.034	0.698AsnGln: 0.698 ± 0.024	1.656AsnArg: 1.656 ± 0.03	1.163AsnSer: 1.163 ± 0.026	1.365AsnThr: 1.365 ± 0.033	1.869AsnVal: 1.869 ± 0.036	0.421AsnTrp: 0.421 ± 0.015	0.592AsnTyr: 0.592 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	7.198ProAla: 7.198 ± 0.072	0.321ProCys: 0.321 ± 0.014	4.582ProAsp: 4.582 ± 0.059	3.695ProGlu: 3.695 ± 0.052	1.668ProPhe: 1.668 ± 0.03	5.483ProGly: 5.483 ± 0.06	1.249ProHis: 1.249 ± 0.029	2.18ProIle: 2.18 ± 0.038	1.327ProLys: 1.327 ± 0.032	4.958ProLeu: 4.958 ± 0.059	1.297ProMet: 1.297 ± 0.025	1.355ProAsn: 1.355 ± 0.026	3.913ProPro: 3.913 ± 0.09	1.959ProGln: 1.959 ± 0.034	3.439ProArg: 3.439 ± 0.049	3.086ProSer: 3.086 ± 0.055	3.51ProThr: 3.51 ± 0.045	5.116ProVal: 5.116 ± 0.061	0.842ProTrp: 0.842 ± 0.021	1.256ProTyr: 1.256 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.722GlnAla: 3.722 ± 0.049	0.238GlnCys: 0.238 ± 0.012	1.332GlnAsp: 1.332 ± 0.029	1.213GlnGlu: 1.213 ± 0.03	1.043GlnPhe: 1.043 ± 0.024	2.004GlnGly: 2.004 ± 0.037	0.766GlnHis: 0.766 ± 0.024	1.564GlnIle: 1.564 ± 0.029	0.688GlnLys: 0.688 ± 0.021	3.533GlnLeu: 3.533 ± 0.048	0.773GlnMet: 0.773 ± 0.022	0.675GlnAsn: 0.675 ± 0.021	2.02GlnPro: 2.02 ± 0.041	1.331GlnGln: 1.331 ± 0.033	2.909GlnArg: 2.909 ± 0.048	1.485GlnSer: 1.485 ± 0.033	1.704GlnThr: 1.704 ± 0.031	2.533GlnVal: 2.533 ± 0.04	0.604GlnTrp: 0.604 ± 0.017	0.701GlnTyr: 0.701 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.17ArgAla: 8.17 ± 0.082	0.616ArgCys: 0.616 ± 0.021	4.33ArgAsp: 4.33 ± 0.053	3.853ArgGlu: 3.853 ± 0.06	2.523ArgPhe: 2.523 ± 0.038	5.172ArgGly: 5.172 ± 0.06	1.757ArgHis: 1.757 ± 0.032	3.556ArgIle: 3.556 ± 0.051	1.645ArgLys: 1.645 ± 0.032	7.355ArgLeu: 7.355 ± 0.074	1.87ArgMet: 1.87 ± 0.034	1.74ArgAsn: 1.74 ± 0.029	3.953ArgPro: 3.953 ± 0.052	2.373ArgGln: 2.373 ± 0.04	6.58ArgArg: 6.58 ± 0.075	3.845ArgSer: 3.845 ± 0.047	4.112ArgThr: 4.112 ± 0.052	5.589ArgVal: 5.589 ± 0.059	1.364ArgTrp: 1.364 ± 0.031	1.962ArgTyr: 1.962 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	6.826SerAla: 6.826 ± 0.064	0.428SerCys: 0.428 ± 0.017	3.268SerAsp: 3.268 ± 0.043	2.597SerGlu: 2.597 ± 0.039	1.612SerPhe: 1.612 ± 0.033	5.345SerGly: 5.345 ± 0.064	0.983SerHis: 0.983 ± 0.021	2.15SerIle: 2.15 ± 0.033	1.125SerLys: 1.125 ± 0.027	4.559SerLeu: 4.559 ± 0.056	1.387SerMet: 1.387 ± 0.028	1.078SerAsn: 1.078 ± 0.027	3.046SerPro: 3.046 ± 0.048	1.538SerGln: 1.538 ± 0.036	3.583SerArg: 3.583 ± 0.049	2.936SerSer: 2.936 ± 0.045	3.343SerThr: 3.343 ± 0.056	4.512SerVal: 4.512 ± 0.056	0.824SerTrp: 0.824 ± 0.026	1.216SerTyr: 1.216 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	7.826ThrAla: 7.826 ± 0.078	0.432ThrCys: 0.432 ± 0.017	3.827ThrAsp: 3.827 ± 0.05	3.294ThrGlu: 3.294 ± 0.045	1.917ThrPhe: 1.917 ± 0.037	5.792ThrGly: 5.792 ± 0.065	1.122ThrHis: 1.122 ± 0.026	2.456ThrIle: 2.456 ± 0.042	1.294ThrLys: 1.294 ± 0.026	5.562ThrLeu: 5.562 ± 0.057	1.326ThrMet: 1.326 ± 0.031	1.252ThrAsn: 1.252 ± 0.032	3.869ThrPro: 3.869 ± 0.048	1.507ThrGln: 1.507 ± 0.035	3.642ThrArg: 3.642 ± 0.049	3.264ThrSer: 3.264 ± 0.05	3.917ThrThr: 3.917 ± 0.067	6.025ThrVal: 6.025 ± 0.07	0.851ThrTrp: 0.851 ± 0.023	1.386ThrTyr: 1.386 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	11.403ValAla: 11.403 ± 0.103	0.76ValCys: 0.76 ± 0.022	5.959ValAsp: 5.959 ± 0.066	4.762ValGlu: 4.762 ± 0.06	2.693ValPhe: 2.693 ± 0.049	7.08ValGly: 7.08 ± 0.07	1.79ValHis: 1.79 ± 0.037	4.016ValIle: 4.016 ± 0.051	1.807ValLys: 1.807 ± 0.04	8.882ValLeu: 8.882 ± 0.088	1.735ValMet: 1.735 ± 0.035	2.222ValAsn: 2.222 ± 0.036	4.662ValPro: 4.662 ± 0.053	2.242ValGln: 2.242 ± 0.036	5.929ValArg: 5.929 ± 0.056	4.724ValSer: 4.724 ± 0.048	5.67ValThr: 5.67 ± 0.068	8.629ValVal: 8.629 ± 0.087	1.076ValTrp: 1.076 ± 0.026	1.672ValTyr: 1.672 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.586TrpAla: 1.586 ± 0.032	0.169TrpCys: 0.169 ± 0.009	0.82TrpAsp: 0.82 ± 0.021	0.665TrpGlu: 0.665 ± 0.021	0.52TrpPhe: 0.52 ± 0.018	0.997TrpGly: 0.997 ± 0.025	0.378TrpHis: 0.378 ± 0.017	0.645TrpIle: 0.645 ± 0.022	0.322TrpLys: 0.322 ± 0.014	1.745TrpLeu: 1.745 ± 0.038	0.356TrpMet: 0.356 ± 0.012	0.417TrpAsn: 0.417 ± 0.017	0.842TrpPro: 0.842 ± 0.026	0.645TrpGln: 0.645 ± 0.019	1.318TrpArg: 1.318 ± 0.027	0.921TrpSer: 0.921 ± 0.024	0.931TrpThr: 0.931 ± 0.025	1.147TrpVal: 1.147 ± 0.026	0.328TrpTrp: 0.328 ± 0.016	0.339TrpTyr: 0.339 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.426TyrAla: 2.426 ± 0.041	0.279TyrCys: 0.279 ± 0.014	1.446TyrAsp: 1.446 ± 0.032	1.126TyrGlu: 1.126 ± 0.03	0.836TyrPhe: 0.836 ± 0.022	2.096TyrGly: 2.096 ± 0.039	0.484TyrHis: 0.484 ± 0.017	0.711TyrIle: 0.711 ± 0.022	0.401TyrLys: 0.401 ± 0.016	2.37TyrLeu: 2.37 ± 0.036	0.309TyrMet: 0.309 ± 0.014	0.508TyrAsn: 0.508 ± 0.019	1.33TyrPro: 1.33 ± 0.032	0.752TyrGln: 0.752 ± 0.022	1.937TyrArg: 1.937 ± 0.035	1.124TyrSer: 1.124 ± 0.023	1.249TyrThr: 1.249 ± 0.033	1.724TyrVal: 1.724 ± 0.037	0.374TyrTrp: 0.374 ± 0.015	0.527TyrTyr: 0.527 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5158 proteins (1625613 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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